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Tear Jerker / Lucky Star

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Lucky Star! You're supposed to make me laugh, not bawl my eyes out!
  • In general, anything involving Kanata Izumi, the deceased mother of Konata Izumi, often brought out the Kleenex for fans of the anime.
    • The singlehandedly fact that Konata lost her mom at a very young age, neither the little otaku having almost any memories of being with her.
    • Pictured above, before Kanata's scene there is a quick picture of Soujiro mourning her dead, alongside Konata, who was still a baby at the moment when her mom died.
    • As the spirit of Kanata visits her family, she laments that despite accepting her death, she can't be there for her family, especially her daughter.
    Kanata Izumi: The fact that I can no longer be there hurts a little.
    • Soujiro and Kanata's reminiscence of their life together when the latter was alive ranks as easily in the top five most sincerely heartwarming, not to mention heartbreaking, moments in 2000s anime.
    • Kanata's Image Song, "Shiawase Negau Kanata kara" ("Wishing for Your Happiness from Kanata/the Other Side") also qualifies.
  • Nanako, who is single at 27, buys a discounted Christmas cake after the 25th of december upon remembering her students discussing the Christmas cake/unmarried woman metaphor of women not being desirable after reaching 25, ending with her walking towards the sunset claiming that neither the cake nor she are unwanted.
    Nanako Kuroi: "Doesn't count as a leftover. 'Cause I bought one."
  • "My people... are everywhere." Cue Manly Tears.
  • There's also the scene between Kagami and her older sister, Matsuri. When Kagami doesn't get a message about getting some supplies, Matsuri lays into the twins. Kagami storms off to get the supplies, and Inori Hiiragi pointedly tells Matsuri that she could have handled it better, and Matsuri agrees.
  • During the class trip, Kagami gets a note from a guy to meet her at a certain place at night. From the way she was acting, she was expecting it to be a love confession. But it turns out all the guy wanted to do was ask her if she would part with the keychain she'd bought earlier that day because he thought it was cute. You have to feel bad for her.
  • Near the conclusion of the last episode, the girls looking with sadness at the night preparations of the school's festival, realizing that this will be one of their last times having fun together before graduating, while a slow, sad version of the opening sounds.
  • The OVA
    • Minami worries about Cherry's health in the beginning of the Ova, as the dog refuses to eat the food she's prepared, and leaving the poor girl in tears when Minami notices that Cherry ate food that the dog previously buried in the garden.
    • For Tsukasa fans, the volleyball scene. Her sister smacks her in the face with the ball, she tries to give it her all for once, but she still loses the game. One could reasonably argue that it wouldn't really be Tsukasa if she won, but still. She says, "Next time...", but given the "grand finale" feel to the OVA, it doesn't seem like there will ever be a next time outside the realm of fanfiction.