A-Team Firing: No matter how many shots are fired (and no matter how easy the shot is), the number of times someone is actually shot throughout the series can probably be counted on one hand, almost all in the last episode.
Beat Them at Their Own Game: Chaynah, chief of the Comanche, is well-read on US law in general and the treaty between his people and Washington in particular. When a government representative is sent to move them to a new territory, he is able to explain how there is no legal cause for the action.
Becoming the Mask: Liam Canary, Jane's father, spent so much time talking about his family that a Con Man acquaintance of his decides to do his best to live that life.
Blasting It out of Their Hands: When Jane is working with a bow and arrow, she shoots the guns out of the hands of a pair of assailants (Launching two arrows at the same time) and later knocks the canon-lighting-torch out of the hands of another assailant.
Bowdlerization: Jane's past is both toned down from her historical counterpart (who was both a heavy drinker and a whore, among other things), but was still plenty unusual for the medium (young cabaret singer, though Sue says she was thankfully handled with "kid gloves").
Casual Danger Dialogue: Joe is purely perplexed at Jane and Wild Bill Hickock calmly talking about playing cards when they are under seige by twenty outlaws.
Con Man: Liam Canary from "Like Father, Like Daughter". He pretended to be Jane's long lost father to keep her distracted while his possé tried to rob Deadwood's bank. Later he decided that, even if it was not the truth, he wanted it to be.
Conveniently an Orphan: Jane's father has been missing since her early childhood, and the implication from "Like Father, Like Daughter" is that her mother died after he disappeared.
Crazy-Prepared: Jane has a knife hidden in the handle of her whip, which she uses to cut herself free after she has ben tied up with her own whip.
Friendly Enemy: Sam Bass, a thief and hired gun, brought Jane a friendly glass of milk when he learned she was in the same saloon as he, and sat down with her at a table to give her all the information on the fugitive she was chasing, including the backgrounds on the gunmen with him, warning her of the danger she would be in if she still tried to capture him. At the end of their conversation, he explains that he is with the gang and again tries to get her to just leave the situation alone.
Gatling Good: One bad guy acquires a Gatling gun and assumes this makes him unstoppable. He is wrong, of course.
Getting Crap Past the Radar: The saloon that Jane lives in is also a brothel; the serving women are seen leading men up to the rooms on more than one occasion, but are never explicitly referred to as prostitutes.
Gun Porn: The animation is extremely detailed when it comes to the operation of firearms. Rifles are levered after each shot, revolvers are loaded one round at a time while rotating the chamber, and shotguns are broken to remove spent shells.
Improvised Weapon: When party-goers are held hostage by Southern troops and Jane is without her usual arsenal, she fashions a bow and arrows out of tree branches and makes a whip using her own dress. Joe, without his trusty shotgun, makes a small explosive and knocks out a soldier by pilfering a buffet table of an ear of corn and a banana.
Indy Escape: At least two examples present in the series: One where Jane drives a train off of a slowly collapsing bridge, and one direct shout-out when Captain O'Rourke detonates a TNT charge at the entrance to a canyon, leaving Joe running down a runoff ditch from a large round chunk of debris.
Last-Second Chance: Sam Bass, a thief and hired gun, and Jane are friends who would prefer not to have to fight one another. When Jane is chasing a fugitive wanted for bank robbery and murder, only to learn that Bass is now part of his gang, each tries to get the other to leave and not get involved. At the end of the episode, after Jane has managed to disable most of the gang, she tells Sam to give up and drop his gun since he is "not a killer." Bass, however, sees this as an opportunity to finally get a Big Score and leave his life of petty thievery behind, and keeps trying to load one more shell into his rifle right up until Jane knocks him out.
My Country, Right or Wrong: Captain O'Rourke of the US Cavalry explains even his unconscionable orders with "I have my orders." Chena of the Comanche says not to hold it against him, since if one does not stick to his principals (In O'Rourke's case, the principal of serving his country) then there is nothing left.
No-Holds-Barred Beatdown: Episode 13. In the span of five minutes Jane had her hands tied, was repeatedly punched in the gut, smashed in the face, thrown into a crate, tossed through a wall and left to die in a desert where vultures tried to eat her. And still there wasn't a single drop of blood. No wonder she's so white.
One Last Job: Sam Bass joins up with Wooden Leg Gibson to rob a US Cavalry fort of weapons and ammunition for the Big Score to be able to leave his life of petty thievery behind. Jane interrupts the theft and gives Bass the chance to walk away. He does not take that chance.
The Remnant: A group of Confederate soldiers still fighting the Civil War plan to assassinate President Grant and re-start the conflict.
Self-Made Orphan: The (very slight) implication in "Easy as One, Two, Three..." is that Conrad killed the mother of the triplets.
Sheathe Your Sword: When the US Cavalry comes to move the Comanche, whether the Comanche want to be moved or not, Chaynah has all the warriors arm themselves and prepare for battle, only to toss his bow and arrows on the ground before the cavalry captain. As he explains, the treaty between the Comanche and the US allows them to live on this land for as long as they keep the peace; by refusing to fight, the Comanche force the US government to break its own laws in order to relocate them.
Spotting the Thread: Jane realizes that the troop of cavalry guarding a fair are imposters after she notices all the minor discrepencies, starting when the guard at the gate does not quite seem to understand just how to close the gate.
Stating the Simple Solution: When the triplets have captured Jane they are planning to run her down with horses and beat her to death, and Conrad asks why they do not just shoot her. Unfortunately for all of them, his brothers do not heed his advice.