Colbert Bump: T.E.C. 3001 has gotten a fair bit of publicity thanks to their ringing endorsement of the game in the second Xbox Live Indie Games video, prompting the developers to publicly thank them.
Creator Backlash: Downplayed. After 11 episodes, they decided to end the Mailbag segments indefinitely, not out of anger (they make it clear throughout the episodes that they adore their fans), but because they had become so overwhelmed with gifts that they were running out of room in their homes.
Fake Nationality: Slowly averted as time went on. The guys initially passed themselves off as American, but over time they revealed their real, Canadian nationality.
I Knew It: Happens a lot during their Beyond: Two Souls playthrough as more and more of their predictions about the plot come true. The biggest of these, however, comes right at the end from Woolie, for guessing that Aiden was really Jodie's unborn twin brother less than halfway through the game.
Old Shame: Fighterpedia. A series that lasted one season before Two Best Friends properly took off featuring Matt and Woolie discussing the intricacies of fighting games. To sum up Matt's feelings about it:
Woolie:*picking his character* Representin' that 'Pedia!
Matt:*laughing* No one cares about Fighterpedia, not even me!
*cue title footage of Fighterpedia mentioning how much it sucked*
Pat: It came out! Matt: No... Pat: It came out! Matt: Okay, you encoded something on a disc, and you put it in here to troll me. ... Pat: Downloadable content?! They still didn't finish the whole game!
Vitriolic Best Buds: Although they're good friends now, it is revealed in a interview that the two strongly disliked each other when they first met.
What Could Have Been: When Matt came up with the idea for the original video, Two Best Friends Play Kirby's Epic Yarn, he wanted it to be himself and Woolie. Woolie thought the idea was stupid, and declined to take part. Pat was Matt's second pick, even though they weren't even very good friends at the time.
Woolie has gone on record saying the reason he turned it down was because he was busy watching Street Fighter IV streams when asked, and at the time didn't think the idea was worth spending his time on. Nowadays he jokes that he semi-regrets that decision.
Apparently, there was going to be a Bioshock Infinite episode, but due to the controversial content, they found it difficult to approach without sounding insensitive.
There was a "Super Best Friends Watch" of Metal Gear Solid 4, and it even had some episodes made, but they were forced to drop it when Konami threatened to sue.
The same applies to the Super Best Friends playthrough of New Super Mario Brothers U, which was halted due to Nintendo's changing policy toward videos on Youtube. (They now have full monetization rights for any videos using content from their games)
An Asura's Wrath Let's Watch was stopped as well.
As Pat revealed on Retsu Talk, they were gonna do a Peace Walker lets play, but unfortunately Konami threatened to sue yet again.