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• Fanservice: Sam gets his first sex scene! Huzzah!
• Fourth Date Marriage: Admittedly, Sam and Madison just have sex with one another after a day or two of flirting—which is pretty par the course for Supernatural's Love Interests—but in "The Monster At the End of This Book", it's said that Madison is the first woman Sam falls in love with after Jessica. Within two days. Mind you, this was coming from an overzealous fan-girl, but it's implied that Chuck's Supernatural books have more details than shown on-screen and might offer more insight into Sam's thoughts, so it's very possible that she was telling the truth.
• Downer Ending: Sam and Dean can't cure Madison, so she asks Sam to kill her. They go off-screen while a tearful Dean watches on, "Silent Lucidity" by Queensr˙che plays—then BOOM, gun shot, Dean flinches, credits roll.
• Girl of the Week: The lawyer's Sexy Secretary, Ms. Madison, who is also the Monster of the Week.
• Hey, It's That Guy!: The medical examiner is Doctor Fraiser
• I Cannot Self-Terminate/Staking the Loved One: In the ending scene, Madison learns that there is no cure for being a werewolf, and begs Sam to kill her so that she won't hurt anyone else. He obliges.
• It's Personal
• Only One Name: Madison.
• Our Werewolves Are Different: This is their first episode featured on Supernatural, which portrays them as normal people infected by another werewolf's bite. They grow fangs and claws and turn feral after falling asleep, with their human mind unconscious as the werewolf in them acts on their instincts combined with their human self's feelings, i.e. killing those they think are immoral or deem a threat, and trying to turn the person they have feelings for into another werewolf to get a mate. They're injured by silver, and can be killed with the traditional "silver bullet to the heart".
• Single Tear: Dean starts crying when Sam leaves to kill Madison.
• Yank the Dog's Chain: Turns out that killing the sire doesn't cure a werewolf, after all.