An arguably better example along these lines would be the four Alien films, not just in terms of their respectives tones but in light of their respective directors and the directors' general filmmaking styles: Alien is phlegmatic, Aliens is choleric, Alien│ is melancholic and Alien: Resurrection is sanguine.
Alice in Wonderland: Alice and Mock Turtle (sanguine), March Hare and Knave of Hearts (choleric), Dodo and Red Queen (choleric/melancholic), Pigeon (melancholic), White Queen and White Rabbit (phlegmatic/melancholic), Mad Hatter and the Gryphon (sanguine/choleric), and Dormouse and Cheshire Cat (leukine).
The Italian movie Amici Miei and its sequels: Mascetti (choleric), Necchi (melancholic), Melandri (sanguine), Sassaroli (phlegmatic), and Perozzi (leukine).
Melancholic: Ken Mattingly, intended Command Module pilot.
Phlegmatic: Jack Swigert, backup CM pilot who ends up replacing Ken on the actual mission.
The Avengers: The five male members and even their most distinctive colors fit a Combo Ensemble: Tony Stark/Iron Man (choleric gold + sanguine red), Thor (choleric red and yellow + melancholic black), Bruce Banner (melancholic/phlegmatic, but green only when he turns Hulk, ironically); Steve Rogers/Captain America (phlegmatic blue + sanguine red), and Clint Barton/Hawkeye (leukine, neutral grey/black).
The S.H.I.E.L.D. agents: Phil Coulson (phlegmatic), Nick Fury and Natasha Romanoff (choleric), Maria Hill (melancholic), and Clint Barton (leukine).
Baby's Day Out: Eddie (choleric), Norby (sanguine), and Veeko (phlegmatic).
The Back to the Future film franchise: Marty McFly (sanguine/choleric), George McFly and Jennifer Parker (phlegmatic/melancholic: opposite of Marty), Goldie Wilson (sanguine/choleric), Biff Tannen (choleric), Clara Clayton (melancholic), Mr. Strickland (choleric/melancholic), Doc Brown and Lorraine McFly (sanguine).
The McFly family: Lorraine (sanguine), George (phlegmatic), Dave (choleric), Linda (melancholic), and Marty (sanguine/choleric).
The Big Lebowski: kind of. The Dude is the epitome of phlegmatic, as is Walter to choleric and Jesus Quintana to sanguine; Donny is arguably either phlegmatic or melancholic.
Braveheart: William Wallace (choleric), Campbell the Elder (melancholic), Hamish Campbell (leukine), and Stephen the Irishman (sanguine).
The Breakfast Club: John Bender (sanguine), Claire Standish (choleric), Brian Johnson (melancholic), Andrew Clark (phlegmatic), and Allison Reynolds (leukine).
Cars: Lightning McQueen and Tow Mater (sanguine), Sheriff (choleric), Doc Hudson (melancholic/choleric), Red (melancholic), Sally Carrera (phlegmatic), Strip "The King" Weathers (sanguine/choleric), and Mack (leukine).
Charlie's Angels: Natalie (sanguine, or alternatively phlegmatic), Dylan (choleric), Alex (melancholic), and Bosley (phlegmatic, or alternatively sanguine).
Chronicle: Andrew (melancholic), Steve (sanguine), and Matt (starts out phlegmatic but becomes more choleric as the story progresses).
Cinderfella: Rupert (choleric), Cinderfella (melancholic), Princess Charming (phlegmatic), Maximilian (sanguine), and the stepmother (leukine).
Clerks: Randall (sanguine), Jay (choleric), Dante (melancholic), and Silent Bob (phlegmatic).
Courageous: The four main cops: Adam Mitchell (choleric), Shane Fuller (choleric), Nathan Hayes (melancholic), and David Thomson (phlegmatic), plus their non-cop friend Javier Martinez (sanguine).
The Croods: Gran (choleric), Grug (melancholic/choleric), Eep (melancholic), Ugga (phlegmatic/melancholic), Thunk (sanguine), Sandy (sanguine/choleric), and Guy (leukine/phlegmatic).
The villains of The Dark Knight Saga: Henri Ducard aka Ra's Al Ghul & Miranda Tate aka Talia Al Ghul (melancholic), The Scarecrow (phlegmatic), Harvey Dent aka Two Face & Bane (choleric), and The Joker (sanguine).
Despicable Me, though it isn't until the sequel that the ensemble is completed: Gru and Margo (melancholic), Edith (choleric), Agnes (phlegmatic), and Lucy (sanguine).
Diary of a Wimpy Kid: Frank Heffley (sanguine/choleric), Rodrick (choleric), Greg (melancholic), Susan (phlegmatic), and Rowley (sanguine).
The villains of the Die Hard films: Hans and Simon Gruber (choleric), Col. Stuart (melancholic), and Thomas Gabriel (phlegmatic).
Elf : Buddy (sanguine), Walter Hobbs (choleric), Michael Hobbs (melancholic), Jovie (phlegmatic).
From The Emperor's New Groove, Combo Ensemble: Kuzco (choleric/sanguine), Yzma (melancholic/choleric), Pacha (phlegmatic/melancholic), and Kronk (sanguine/phlegmatic).
Enchanted: Giselle (sanguine), Edward (choleric), Robert (melancholic), Nancy (phlegmatic).
Facing the Giants: The 3 main football players; Matt Prater (choleric/melancholic), Brock Kelly (sanguine/choleric), and David Childers (phlegmatic/melancholic). The 3 coaches/coach assistants: Grant Taylor (choleric/melancholic), Brady Owens (melancholic/phlegmatic), and JT Hawkins Jr. (sanguine/phlegmatic).
The Faculty: Delilah (choleric), Casey and Stokely (melancholic), Zeke (phlegmatic), Stan (sanguine), and Marybeth (leukine until she reveals herself to be the Queen alien).
Fireproof: The five firemen, Combo Ensemble. Caleb Holt (choleric/melancholic), Eric Harmon (melancholic/phlegmatic), Michael Simmons (phlegmatic/sanguine), Wayne Floyd (sanguine/choleric), and Terrell Sanders (leukine).
The coworker women: Deidra Harris (choleric), Catherine Holt (melancholic), Robin Cates (phlegmatic), Latasha Brown (sanguine), and Ashley Phillips (leukine).
Flintstones: Fred Flintstone (sanguine), Betty (choleric), Wilma Flintstone (melancholic), and Barney Rubble (phlegmatic).
Pulp Fiction: Jules (choleric), Vincent (melancholic), Butch (phlegmatic), and Mia (sanguine).
Reservoir Dogs: Mr. Pink (choleric), Mr. Blonde (melancholic), Mr. Orange (phlegmatic), and Mr. White (sanguine).
The Room is a good example of the four central characters manifesting all of the negative aspects of their respective humours, and none of the positive ones: Lisa (choleric), Johnny (melancholic), Mark (phlegmatic), and Denny (sanguine).
The Shrek franchise: Shrek (choleric/melancholic), Princess Fiona (melancholic/phlegmatic), Puss in boots (phlegmatic/sanguine), Donkey (sanguine/choleric), and Gingerbread Man (leukine).
Singin' In the Rain: Cosmo Brown (sanguine), Don Lockwood (melancholic), Lina Lamont (choleric), and Kathy Selden (phlegmatic).
The adults: Maria von Trapp (sanguine/choleric), Captain Georg von Trapp (melancholic/choleric), Baroness Elsa von Schraeder (melancholic), Max Detweiiler (sanguine).
The children: Lisel and Louisa (sanguine), Friedrich and Brigitta (melancholic), Kurt (choleric), Marta and Gretl (phlegmatic).
The nuns: Mother Abbess and Sister Sophia (phlegmatic), Sister Margaretta (melancholic), and Sister Berthe (choleric).
Other: Rolfe (sanguine/choleric), and Herr (melancholic).
Stand by Me: Chris Chambers (choleric), Gordie LaChance (melancholic), Teddy Duchamp (sanguine, although he mostly displays the more negative traits, such as impulsiveness, self-indulgence and being a space case), Vern Tessio (phlegmatic).
A case could be made for this in the new Star Trek movie:
Choleric: Kirk, the cocky fly-by-the-seat-of-his-pants tactical genius.
The three generations of men from the Sonnenschein family played by Ralph Fiennes in Sunshine: The narrator's grandfather Ignatz (choleric/melancholic), the father Adam who pretends to be sanguine (is really melancholic), and Ivan himself (phlegmatic).
Super 8: Charles (choleric), Martin (melancholic), Joe (leukine), Cary (sanguine), and Preston (phlegmatic).
The Three Stooges: Moe (choleric), Larry (melancholic), Shemp (phlegmatic), and Curly (sanguine).
In Real Life, they were different: Shemp (choleric), Moe (melancholic), Curly (phlegmatic), and Larry (sanguine).
Tinker, Tailor, Soldier, Spy: The suspected "moles" — George Smiley (melancholic), Bill Haydon (phlegmatic), Roy Bland (leukine), Toby Esterhause (sanguine), and Percy Alleline (choleric).
Titanic: Rose (phlegmatic), Jack (sanguine), Cal (choleric) and Ruth (melancholic).
UHF: Harvey (choleric), Teri (melancholic), George (phlegmatic), Stan (sanguine), and Bob (leukine).
The Untouchables: Eliot Ness (choleric), Jimmy Malone (melancholic), Oscar Wallace (phlegmatic), and George Stone (sanguine).
V for Vendetta has this with the main opposers to the Norsefire government: V (choleric), Finch (melancholic), Evey (phlegmatic), and Gordon (sanguine).
Waitress: Apart from the protagonist Jenna, the other five main characters follow this scheme.
Choleric: Earl (showing all the negative aspects and almost none of the positive ones). Also Cal is choleric, and a more equilibrate one.
X-Men First Class: Xavier (leukine), Havok (choleric), Beast (melancholic), Mystique (phlegmatic), and Banshee (sanguine).
A villainous example: The pragmatic Emma Frost (choleric), the somber and competent Azazel (melancholic), and the quiet, silent Riptide (phlegmatic), and The Faux Affably Evil Sebastian Shaw (sanguine).
You Me And Dupree: Randy Dupree (sanguine), Mr. Thompson (choleric), Carl (melancholic), Mandy (phlegmatic), and Neil (leukine).