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  • Any scene involving IF getting angry over her bike being 'modified'. There are two notable mentions:
    • Before IF sets off to the past, Historie tells IF that she modified the bike so it can be used to travel through time. Cue to this line from IF after realizing it:
      IF: WHAT? What the hell?! Who gave you permission to modify my F-*bleep*-ing bike?!
    • Upon returning from Sega Saturn Era for the first time, once IF realizes that her bike has been possessed by Neptune and has a new paint job, IF really doesn't take it well, and even gives a Big "WHAT?!". IF spends most of the time grieving over her bike rather than feeling relief that Neptune's alive until Historie snaps her out of it.
  • When IF and Segami catch up with Saturn, Saturn tries to brush them off. However this causes Segami to cry. IF seizes this opportunity, telling Saturn to stop the bullying... and Neptune suddenly shows up just to make fun of Saturn.
    IF: Can you stop bullying her? It's hard enough for me having to babysit her, I don't need to deal with this.
    Saturn: I w-wasn't bullying her. I didn't do—
    Neptune: YOU MADE HER CRY! ARE YOU SURE YOUR NAME IS SATURN? I'M PRETTY SURE IT'S ACTUALLY SATAN!
    Saturn: HEY! I DIDN'T DO ANYTHING!!
    IF: Uh, Nep? Where the hell did you come from?
    Neptune: Oh, I was passing by and it seemed like you all were having a lot of fun. I just wanted to support my local scene.
    IF: Is that smart for you to do? I thought you were on the run from lil' satan over here.
    Neptune: I'm good. I knew the risk. That being said... Later! (leaves)
    Saturn: What?! You came just to make fun of me?!
  • Neptune and Nepgear have not met in this universe. Their first meeting involves Neptune being in the form of IF's bike. Hilarity Ensues as Nepgear's Wrench Wench habits arise and her first desire is to dismantle her sister! She later wonders which of their parents was the bike, and laments that she wasn't born as a bike herself.
  • In a case of Black Comedy, bike Neptune decides that Nepgear is possessed by the Time Eater and, with Segami in tow, runs Nepgear down only to find out she was wrong. Neptune tries to blame Segami, Segami is afraid Nepgear is dead, and IF casually states they can just go back in time again and prevent that from happening.
    • It's never clarified if this Nepgear actually died before the girls go back in time to stop it, driving the moment even further in Dark Comedy territory.
  • The whole war between the CPUs and Sega Hard Girls started because Mega Drive decided that Plutia was unfit to be a goddess. What prompted her to make this decision? Her first encounter with Iris Heart!
  • Somehow, Neptune sounds hammier in this game than any other! Special mention goes to when she said the "SATAN!" line and when she called eggplant "THE FRUIT OF DEMONS!" later on. Her voice actor must've had so much fun recording those lines!
  • Nepbike is a goldmine of innuendo and double entendres about "riding" and "getting on", to the point where it seems she's doing it on purpose. And let's not talk about her mentioning the taboo when she does that on her non-bike self!
  • Some of the girls' ultimate class names are fitting, some are slightly weird, but not too bad, then there's Plutia's Total Queen, Nep's Kangaroo, or IF's NINJA (all caps intentional).

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