These are what we call the 'YMMV items.' Things that some people find in this work. We call them 'your mileage might vary' because not everyone sees these things in the same way. This starts discussions in the trope lists, a thing we don't want. Please use the discussion page if you'd like to discuss any of these items.
Garry also counts. On a Facebook poll, he was voted the most popular villain in the comic.
There's also Coyote. When Lazerbot made a poll asking readers whether they liked Coyote, literally every vote said they liked him.
Don't forget Underbite Troll. He got popular enough to have a miniature spin off.
Fridge Horror: In Donkey Kong's first appearance in the comic, he was shown sleeping after having a threesome with Knuckles and Rogue. In his most recent appearance, he's prevented from helping the Troll Slaiyers out because Rogue gave him Gonnorhea. No points for deducing what this means for Knuckles.
God-Mode Sue: The Troll King skirts dangerously close to this trope thanks to his Seven Deadly Curse Words, that would let him easily steamroll almost the entire cast if it weren't for a severe case of Genre Blindness that causes him to only use it as a last resort.
It seems to be implied that each of the words can only be used once or they lose power if overused.
Growing the Beard: At first, the comic gained attention due to how ridiculously bad it was. But as the story went on, the characters become more rounded, and Lazerbot's art improved drastically, and as a result, many people have actually become invested in the story, and have grown to like it unironically. It's difficult to pinpoint an exact moment at which people actually started caring about the story, but many will proclaim the 4th chapter, to be the point where the comic starts making big improvements, quality wise.
It Gets Better: At first, it seems an awful lot like a hilariously bad revenge fantasy, with people like Mario, Sonic and Shadow killing annoying yet harmless trolls. Then, the comic actually gains some character depth when the characters enacting the revenge fantasy are shown to be as terrible, horrible people who are just as bad as the people they fight in the story, at the same time having the villains of the story get stronger and have more characterization - you can't really call it a revenge fantasy anymore. Don't be fooled (There's a reason why this is YMMV), at the end of the day, we're still talking about a Sonic the Hedgehog crossover comic done in MS Paint, but the comic's come a long way when compared to its beginnings.
Memetic Mutation: Probably the reason why it has gotten so much attention on the internet is for how hilariously bad it is, and the fact that said hilarity made some memetic mutations. Many panels from the comics have been used as reaction images on imageboards and avatars on forums. We could sit and discuss them, but we'd be here forever, so for the really well-known ones...
"I'm so mad. im gonna have sex with my girl friend so I wont be so mad"
In one panel, Bugs Bunny is shown with the caption "the gay". It only took a few moments to guess how memetic the phrase eventually became.
"High shovel" has also became a popular meme.
Nightmare Fuel: Dr. Eggman raping Amy to the point where she attempted suicide, not at all played for laughs.
Teasing Creator: Lazerbot, all the time. The most glorious example was his fake killing of Rob - what followed was a text-only pannel where Lazerbot explained that basically no one is safe, fan-favorite or not.
Viewer Gender Confusion: Alex the Emo Troll. Some people unknown with the comic have commented on him being a girl.
The Woobie: Tails. The title says it all, "Tails gets trolled." He also gets beaten down constantly by the trolls, gets ridiculed by them, and loses his best friend Sonic to the trolls.
Porky Pig. He's a religious fellow that got involved in a ton of bad events, eventually making him one of the Neutrals. When the Church he is a part of finds out, they see him as evil incarnate and kill his father and his mother before he has a chance to see them, and then murder his wife and child in front of him. It's no wonder that he is Driven to Suicide.
Mario and Luigi. One of the biggest moments of Mario's flashback is them meeting their parents, only to be told that they aren't loved and that their father just enjoys having unprotected sex. Luigi more so, because he was mostly dragged into doing all of this for the sake of his brother.