In Frisky Dingo, Big BadStan is forced at gunpoint to dance to disco music and shout "Master cylinder!" while wearing nothing but a tall-boy beer can covering his crotch and being pelted with sweatshop-made action figures of himself until he has a heart attack...only to be revived with a defibrillator * His weiner jumped! and the process started anew.
It should be noted that "Master Cylinder" is something Xander/Awesome X does for fun.
"Don't look over here at what I'm doing! You point at it! You celebrate yourself!"
The Grand Finale of Avatar: The Last Airbender: It wasn't enough for Fire Lord Ozai to be defeated. He could have died with honor. That would have been too easy. Instead, the most powerful firebender in the world was brought to justice by a twelve/thirteen year-old boy. If that wasn't already enough, his firebending was taken away from him forever (which more than one fan has referred to as "Spiritual Castration"). In addition to that, he was openly mocked at while lying limp on the ground from exhaustion by a bunch of kids who blatantly laughed at the fall of everything he had established as a ruler. He tries to retort, only to comically collapse face first, butt in the air, drooling on the floor. Oh, but then — ! To cap it all off, he is greeted in the same prison cell used to contain his brother by the very son he banished from the nation, in a total role reversal. What words does he receive? "You should count yourself lucky the Avatar spared your life."Ouch. That conga line was trucking so fast, one wonders why it didn't result into a train wreck.
From the same finale: Azula's mind was slowly broken over the course of a series of betrayals. First her brother, then her two closest "friends", and then finally her father's seeming ostracism. Indeed, the second betrayal was the worst, as Mai absolutely crushed Azula's entire world-view with a single sentence. At the last, when she had beaten her brother in a climactic duel, when she finally had the chance to punish her brother and restore some shred of order to her now chaotic world, she was defeated and humiliated. And who defeated one of the greatest Firebenders? Not a seasoned master of a bending art, who had studied for decades. Not by the Avatar himself. Not by a 12-year-old "Greatest Earthbender in the World." Not even by her own brother, whom she'd just defeated (admittedly by cheating). But by a Southern Water Tribe peasant, who not a year earlier could barely bend a puddle of water. On the day of Sozin's Comet no less, when her firebending was magnified a hundred fold. In the end, Azula was hog-tied to a grating with chains, soaking wet and left to flail around impotently.
The Alpha Bitch, Heather, in Total Drama Island constantly gets humiliated, in some episodes more than others, throughout the series. It all culminated by the end of the series when all of her hair gets shaved off in a razor blade accident, is finally voted off kicking and screaming, and in the very last episode, no one was willing to put up with her anymore due to her cruel treatment of them throughout the show (except Harold). Of course, she arguably had it coming anyway...
Also, having her hair shaved off was suggested by Lindsey as part of the challenge, whom not only Heather utterly manipulated and betrayed, but threatened to cut off HER hair at one point. Total Karma Island.
Minor antagonist, Eva also suffers this in season 1.
Season 2's villain Justin isn't safe either. His good looks begin to lose their charming effect on the ladies, and since that's the only thing he's got going for him, he is understandably depressed. The rest of his time on TDA is spent trying unsuccessfully to cope with his decline in beauty. Things start to look up in The Princess Pride, when he falls for Courtney, and she seems to be interested in him too. Only she's not; she was only playing him in order to snag the easy win in the 11th hour. He gets voted off, and finds that no one is terribly sorry to see him go. Worst of all, he gets shoved aside in Season 3 to make room for Ale-"New Justin"-jandro.
In the same season, Courtney gets a Humiliation Conga of her own. She fails miserably at every challenge after Rock n' Rule, Beth of all people pulls a fast one on her in Crouching Courtney, Hidden Owen, and she eventually gets voted off by her boyfriend. All the while she becomes more and more of a Scrappy.
And Total Drama tops itself in World Tour with Alejandro, Blaineley, and Courtney's defeats. This series seems to like making it's antagonists suffer.
This is taken even further in All-Stars where Scott seems to get unnecessarily hurt on an episodic basis.
In the last episode of Clone High, this happens even more literally than the page header suggests. The villain, his plans collapsed and life at an end, convinces everyone to conga. When they do, he sets them up to be cryogenically frozen, subverting the trope utterly.
The Australian cartoon series Little Elvis Jones and the Truckstoppers (about a redhaired child prodigy guitarist who was abandoned as a baby on a little town truckstop run by a pair of Elvis fanatics) finished with one of these for series villain W.C. Moore. At the end of the first season, Moore (an ultra-greedy rich guy) discovers that a potential deposit of the mysterious mineral "Berkonium" exists under the town, and over the course of the second series he literally bankrupts himself trying to find it, and ends up losing the last thing of value he has, the deed that allows him to claim the money that the titular band earns, in a game of marbles. He ends up the indentured servant of his own former servant, Duncan, even wearing the same drab uniform... and with the same electric shock-delivering watch strapped to his wrist.
Cartman: Ha ha ha ha ha ha, I made you eat your parents!
Cartman FINALLY gets his in the 200 and 201 special. He is kidnapped by the Gingers, publically humiliated by Scott Tenorman this time, and then learns that he's responsible for his own dad's death since Tenorman is his half-brother, meaning that Cartman is half-ginger, something he loathes. All very, very satisfying.
Cartman's most hilarious punishment arguably came in the episode "Awesome-O." Eric disguises himself as a robot (complete with a stereotypical "robot voice") and has himself mailed to Butters's house as an early birthday present for Butters - just so he can spy on Butters and learn embarrassing secrets about the boy that he can tell everyone at school. But the plot backfires spectacularly: not only can Cartman not eat or attend to personal hygiene while wearing the robot disguise, and not only does Butters treat him as his personal slave, but he's mistaken for a real robot and eventually captured by a government agency who try to drill him open to find out what's in his electronic brain. And as if all that weren't humiliating enough, he finally accidentally farts in Butters's presence and exposes himself as a human being - and Butters punishes him for his trickery by showing all the kids in the neighborhood a videotape he secretly made of Cartman dressed as Britney Spears and dancing around and singing to a cardboard cut-out of Justin Timberlake ("Oh, Justin, touch my body!"). The episode ends with all the kids laughing hysterically and Cartman sulking among them with a "Curses! Foiled again!" expression on his face.
Actually, Cartman suffered this in the very first episode, with a massive satellite dish coming out of his rear.
Cartman is treated to yet another spectacular conga in the fifth season episode "Cartmanland". Eric inherits one million dollars (undeservedly) and uses the money buy a failing amusement park, fulfilling his life's dream of having a park all to himself. In true Cartman fashion, he immediately begins running television ads proclaiming that no one is allowed to enter his park--especially Stan and Kyle. Cartman quickly realizes that he can't run the park by himself; however, since he already used all his money to buy the park, he can't afford to pay employees. Reluctantly, Cartman decides to open the park's gates to a limited number of guests, reasoning that a few people won't be a problem in such a big park. However, the guests quickly ruin Cartman's fun, forming a line in front of the very ride he wants to board. Park expenses continue to pile up, forcing Eric to admit more and more people every day. "Cartmanland" quickly becomes a huge success (due largely to Cartman's ad campaign), and soon the park is full of jostling, screaming children (including Kenny, who dies on a roller coaster). Disgusted, Cartman sells the property back to its previous owner for his original million dollars. Immediately thereafter, his money is seized by the IRS to cover the unpaid taxes on the park, and to compensate Kenny's parents for a lawsuit on their son's death. Cartman loses his million dollars, and is left $13,000 debt besides. In desperation, he tries to buy back the property, but the owner refuses—remarking that only an idiot would sell the park now that it's so successful. Stan and Kyle arrive at the park to see a miserable Cartman throwing rocks at the park gates—and getting maced in the face by the security guard who used to work for him.
In almost every episode of Biker Mice from Mars, the Biker Mice level Lawrence Limburger's tower at the end.
In The Simpsons two-parter "Who Shot Mr. Burns?", Moe is interrogated by the police while hooked up to a lie detector. After they run out of questions, Moe says he has to go because he has a "hot date" that night. The machine says this is false, and Moe goes through a few progressively downgraded versions of his evening plans before arriving at the truth: he will spend the night at home leafing through the Sears Catalog.
Moe: Now would you unhook dis already, please?! I don't deserve this kind of shabby treatment! (the machine buzzes)
Nelson Muntz is put through the conga when he laughs at a very tall man in a very small car. First the giant threatens him, bringing him to tears. Then the giant makes him march down the street with his pants around his ankles in front of a huge crowd, the members of whom all laugh at him in the "Haw, haw!" manner Nelson himself always uses. Then he gets squirted with mustard by Bart and Milhouse, who are watching everything happening from a bridge high overhead.
In another episode, Principal Skinner gets a triple dose: after Bart makes a weather balloon look like "Big-Butt Skinner", he's punished by having to help Skinner in his late-night stargazing. Skinner runs off to catch the balloon and while he's gone Bart fools with the telescope and sees something. Skinner gets back just in time to hear the local observatory (on his cell phone) saying "Congratulations Bart, you just discovered a comet!" He lets out a Big "NO!"... which causes him to release the balloon again. He lets out a Bigger No, and then a paperboy drives by and throws out a newspaper that reads "Prez Sez: School is for Losers!", prompting the Biggest No of all.
This frequently happens to Wile E. Coyote in the Road-Runner cartoons. Example: in Zoom And Bored the Coyote fired a harpoon gun at the Road-Runner, only to have his foot snagged by the harpoon cable. He ended up being dragged behind the harpoon along an asphalt highway, through a drainage tube and across a field of cacti, until the harpoon embedded itself directly above a train-tunnel entrance. As he slowly swung down into the tunnel, the Coyote was pushed out by an oncoming train, with his hands still on the harpoon cable, so he ended up swinging to a cliff above the tunnel. His feet finally on solid ground, a relieved Coyote stood gasping for air, as the Road-Runner appeared behind him, preparing to "beep-beep" the Coyote off of the cliff again!. After a beat, the Road-Runner held up a sign reading, "I just don't have the heart." and zipped off, ending the cartoon.
Disney apparently loves using this trope. When they're not in the mood for a scary Karmic Death.
101 Dalmations sneaks in a classic example: after the Badduns ram her into the ditch, you see Cruella bursting into tears of rage amid the parts of their wrecked vehicles while Jasper snarls "Ah, shaddap!" The scene then changes to Roger and Anita listening to his song "Cruella De Vil" on the radio. After he turns it off, Anita remarks that the song was his "first big hit" which "made more money than [they] had ever dreamed of."
It continues in the first part of the 2003 sequel; Cruella's car's been reassembled, but only just enough to still be considered a car. It barely runs anymore, it leaves a trail of parts behind it as she tries to drive it, and finally what's left of it collapses into a heap on top of her. Just to add the final touch to the conga, the first thing to come over the radio when she gets the car to start is... "Cruella De Vil."
Cruella: Shut up! (kicks the radio deck) Shut up! Shut up! SHUT UP!(radio cuts out and falls out of the dashboard) (*groans* *sobs*)
Darkwing Duck's two-parter "Just Us Justice Ducks" ends with Negaduck falling off of city hall, bouncing off of power lines, having his head smashed against heavier and heavier objects to knock him back down, and finally suffering the same fate Darkwing almost suffered at the end of part 1. Oh, except the truck misses. And the switch for the doomsday device breaks on Negaduck's head as he climbs out of the wreckage.
Kuzco was turned into a llama, chased by black jaguars and sent through rapids. And then he fell down an Inevitable Waterfall. Since he was still a total jerk at that point, he might qualify for this too.
Wallace and Gromit - The Wrong Trousers: After catching him, Wallace and Gromit have Feathers paraded into the police station, Shipped in Shackles and towed by the same Techno Trousers he used in the robbery. He then gets imprisoned in a zoo, on display to everyone passing through the entrance.
Adventure Time: The entirety of "Too Young" is this for Lemongrab as Finn and Princess Bubblegum (who happens to be his mother/creator) try to drive him away. First, he received a note that said "YOU REALLY SMELL LIKE DOG BUNS." Then he was approached by the protagonists, who were dressed up as ghosts, punched in the stomach, and pushed onto the floor. We were then treated to a scene of him crying and coughing on the floor for several seconds before awkwardly trying to laugh off their "prank". After that, he gets "spice bombed" when Finn and Bubblegum put a super-spicy serum in everything Lemongrab tries to eat, from a plate of mashed carrots to an apple hanging from a tree to a pile of dirt. And after all of that humiliation, Princess Bubblegum gets her adult body back, and shouts: "YO, EARL! Hey- you're FIRED, ya BUTT!" He rides off into the sunset on his horse, muttering bitterly to himself, obviously upset by the whole thing.
At the end of "Memory of a Memory", after Finn shows Marceline how her ex-boyfriend Ash tricked Finn and Jake into stealing one of her memories for him, Marceline responds to Ash's demand that she make him a sandwich by kicking him in the groin. Then Finn and Marceline continue to kick him when he's down, and to cap things off Jake turns into a Giant Foot of Stomping and squashes Ash.
In the episode "Blood Under the Skin" Finn searches for mystical Armour to prove he is badass to a asshole knight, he ends up going through the "Swamp of Embarrassment" having to run past people showering while calling him a pervert, and have to calm down a crying beast which rocks him like a baby, and finally finds the armour which is "Lady Armour" after every single incident the Knight appears out of nowhere to taunt him telling him what a loser he is.
American Dad! plays with this in "Dope and Faith"; after Stan discovers that his new friend is an atheist, he decides that they must share the same religious beliefs, and tries to convert him by Humiliation Conga. He then proceeds to detonate his house, destroy his business and make his wife a lesbian by brainwashing her, even though he's a really friendly guy.
At the end of The Aristocats, the evil butler Edgar while attempting to lock Duchess and her kittens in a crate bound for Timbuktu, is attacked by Thomas O'Malley and his alley cat gang, who then proceed to release Duchess and the kittens from the crate, then shove Edgar into the crate instead, before finally pushing the crate out the door just in time for the movers to come pick it up and take it away.
He also suffers one of these every time he encounters Napoleon and Lafayette.
There's an infamous Arthur episode called "Arthur's Big Hit" that is pretty much this. Arthur hits D.W. in a fit of rage after she carelessly breaks his model airplane. The aftermath? Arthur ends up grounded for a week and all his friends get mad at him for hitting his sister even when he tries to explain himself, and his parents never found out about the toy plane D.W. destroyed and instead cuddles to her because she's injured. The episode makes this as the worse offense anyone can do to a younger sibling while ignoring the fact that he would have never done it if D.W. had listened to Arthur in the first place. And to add insult to injury, she just got snotty and said the plane was a fragile piece of crap anyways. Broken Aesop indeed.
My Little Pony: Friendship Is Magic, "Griffon the Brush-Off": After Pinkie Pie used a joy buzzer on Gilda at a party, the griffon began suspecting that the pony was trying to get back at her. Following a series of pranks she fell victim to, she eventually snapped and yelled at Pinkie. However, Pinkie Pie only threw the party to improve her attitude. Rainbow Dash revealed herself to have set up the pranks, and she didn't even intend them all for Gilda. Gilda just happened to set them all off.
Also happens to Twilight when she tries to help Fluttershy wake up the animals in "Winter Wrap-Up". She gets spooked by snakes and bats, chased by bees, and sprayed by skunks.
Another happens to the Alpha Bitch Diamond Tiara, in "Family Appreciation Day" when she makes Applebloom ashamed of her Granny Smith. After her father finds out that his daughter insulted the pony responsible for the family fortune, he makes Diamond Tiara put on a bunny suit and take part in all of the elder Pony's strange rituals for Zap Apples.
Later on in "Ponyville Confidential", after she heavily pushed a cruel gossip column written by the Cutie Mark Crusaders and attempted to blackmail them because they wanted to quit, she was stripped of her editor position and demoted to printer. A job with lots of messy ink to further smear the humiliation.
Let's not forget Captain Hook in the animated version of Peter Pan, especially at the climax. ("I'M A CODFISH!")
Virtually every episode of Phineas and Ferb ends up this way for perennial Butt Monkey Dr. Doofenshmirtz. One carefully-plotted "Inator" after another fails spectacularly, often delivering the Karmic Retribution without Perry the Platypus having to lift a finger.
Regular Show: In "Fortune Cookie" Rigby switches his bad luck with Benson's good luck by switching their fortune cookies, a montage shows Benson getting hit by one bad luck after another, with Benson getting badly beaten up and losing everything, even losing the park to a warlock.
At the ending of the The Powerpuff Girls episode "A Made Up Story," after managing to beat the villain Blossom then brags about her being the leader means she's always one step ahead of everything. She then slips on a puddle of mud, which causes her to stumble into a can of red paint that blinds her, which in turn makes her crash into a bag of flour that pours all over her, and finally crashes into a box of Styrofoam that gets stuck to her face.
Being a Sadist Show, this will happen to any of the 8 house guests in Drawn Together, but special mention goes to Toot, Captain Hero, Xandir, and Princess Clara. Even the Jew Producer gets some of this in the movie, after spending the last two seasons forcing the cast to do humiliating stunts and editing the material for his and the watching audience's amusement.
This happens to Squidward several times in Sponge Bob Square Pants, but especially after The Movie. Plankton and even Spongebob himself have also been subject to this.
In the Dreamworks Film "Rise of the Guardians" Pitch Black not only discovers that the hero he killed is revived and so powerful he gets his ass totally whooped, but also he gains his power from the fear of children, whom emboldened by The Guardians are not only not afraid of him, but even start pelting snowballs at him, then realizing he has no power left he becomes scared, which his own minions sense and Heel-Face Turn on him.
In T.U.F.F Puppy, sick of Snaptrap abusing him constantly Larry quits D.O.O.M and takes the rest of the minions with him, and creates G.L.O.O.M a much much more successful villain organization with plans that actually make sense, are competent, and they don't tell the heroes their plans in advance. (Which was a daily routine with Snaptrap) to further salt the wound, Larry brags to Snaptrap every day about how much better a villain leader he is, as well as their headquarters is in Snaptrap's mother's house.
Various villains in episodes of Rocket Robin Hood have suffered the Humiliation Conga, but perhaps the best example occurs in the episode where Robin and a team of scientists defeat Dr. Medulla by destroying his living planet. Afterwards, when the mad scientst tries to escape, Robin blocks his way, and when Medulla tries to pull a laser gun on him, the hero easily overpowers him with a makeshift quarterstaff. Then Medulla is hung up on a pole to be pestered by a woodpecker that pecks him on his bald head, while Robin and his friends look on amused, laughing and making jokes at the villain's expense.