Fred the squirrel. His literal-mindedness turns all of his dialogue into pure funny — especially in "Otter Things Have Happened," when Marlene (who is dating him) doesn't realize at first that Fred's not trying to be funny.
Marlene: What does Fred like? What does he dislike?
The other penguins pretending to fight the Skorka to appease Private, especially Kowalski being "dragged away".
Julien getting scared of "Two-Scoopula," an inflatable shaped like an ice cream cone, at the end of the same episode.
The end of "Tagged," where the scientist shows the new examples of penguin communication: the "up high," the "down low" and the "too slow."
The end of "Crown Fools," where the penguins and Marlene realize that they have gone through some tough tasks to recover Julien's crown, only to realize after the fact that he had a spare. Rico spits up a crowbar, which Marlene then grabs and chases Julien with.
"No, I will not suck seed. No one will be sucking seed!" manages to be an epic Getting Crap Past the Radar and a CMoF at the same time.
Seconds later, he has another: "We shall see who is ridiculous when popcorn begins exploding from my bottom!"
From the same episode, contrast the penguins and the lemurs methods of getting the popcorn. The lemurs sit on top of them like their popcorn eggs, and the penguins interrogate a kernel.
Also, Skipper ealizing that he shouldn't have installed deadly spikes on the roof of the hideout.
"Buffalo Fire Fighters." Oh my.
"Baby Fat Flingers?"
"Tell me we came out of the MOUTH." -In full deadpan mode.
"It's About Time" had loads of these.
Future Kowalski #2: Skipper! You've got to stop me!
Skipper: (kicks him)
Future Kowalski #2: (falls over) Ow!
Skipper: (deadpan) There. You're stopped.
All the funnier because Private pulled something similar earlier.
Kowalski's reasoning for naming the Chrono-Tron as thus instead of just a time machine as the others suggested:
Kowalski:Oh, sure. And while we're at it, let's call the Great Wall a "fence," the Mona Lisa a "doodle" and Albert Einstein "Mr. Smarty-pants!!!"
Compare Private and then Skipper's questions about the future. Skipper's ideal future is especially freakish.
Private: Tell me, am I living in a cottage in Nova Scotia, happily married with one egg and another on the way?
Kowalski-A: Uh, no.
Private: Aw... (disappointed)
Skipper: Tell me, does the Earth become a post-apocalyptic wasteland terrorized by roaming bands of irradiated mutants?
Kowalski-B: Uh, no.
Skipper: Aw... (disappointed)
In "Hello, Dollface", Rico wants a voice chip for his doll, so Private gives him one from a unicorn doll that's supposed to parody little girl educational shows (it says stuff like "be true to yourself", "don't hurt others", etc.). After Rico gets angry at it, Julien tells him to listen to whatever the doll says. Hilarity Ensues, culminating in Hippie!Rico beating up the others with Ms Perky.
"I thought hippies were nonviolent!"
"Eh, whaddyagonnado ..." *WHACK*
"Snakehead" is chock full of funny moments. This troper's personal favorite was Skipper's Captain's Log:
"Skipper's log. Crew nervous, edgy, gripped by the icy fingers of fear. They may be planning a mutiny..." Only to turn and see Rico ready to strike with a hammer.
In "Cute-Astrophe", when Kowalski dances to try and get the people back to throw food again. And then the looks Skipper and Rico give him... You can't stop looking...
Man: Maybe the Polar Bear's got some new material...
Kowalski: The Polar Bear? *pft* Sure he's the biggest carnivore on land, but can he do this?!
(Boogie music starts to play from nowhere, and Kowalski starts boogeying. Skipper looks exasperated, like he's seen it a dozen times before, while Rico looks mildly nauseated and horrified at the sight)
Kowalski is trying to be cool. Instead he dances like your least cool uncle. On top of everything he moves to slap his own buttocks, but Skipper catches his flipper just in time.
Skipper's reason for the people not caring about their adorable antics.
Skipper: I blame the Internet, with its cheap plethora of puppy videos.
Oh, boy Danger Wears a Cape. Three words: STOP BEING NAUGHTY.
Basically everything Mort does in the Blowhole episode. First there's him riding in the wheel of the bike, then him on the subway, hanging there and growling at the terrified people, the line "I took the subway!", and finally the dancing on the control panel.
The Monty Python-esque randomness of Kowalski's dialogue in "Brain Drain".
Skipper: "Kowalski! Concentrate real hard. How do we help you?"
Kowalski: *concentrates* "Eggs ... are ... egg-shaped! That's funny!"
Another from "Brain Drain:"
Skipper:How did you even manage that?!
Kowalski:[walks by with a plunger stuck to his face] Can't hear you! Toilet stick on face!
It only gets better when Skipper, wanting to keep him out of the way, puts him on "line duty"; i.e., following a circle drawn on the floor "and don't stop until you reach the end".
"Followthelinefollowthelinefollowthelinefollowthelinefollowthelinefollowthelinefollowtheline....I'VE LOST THE LINE!!!"
"I STING YOUR FACE!"
In "The Hoboken Surprise", the gang are attacked by androids, which up till this point passed perfectly for real animals:
Skipper gets amnesia He proceeds to get spirit guides. First was Buck Rotgut. Second was an ancient philosopher... third was Alex the Lion. Shortly after Alex appears, he teaches Skipper how to escape a missile... by dancing. Things only got funnier from there.
At the end of the episode "Love Hurts", we have this exchange;
Skipper: Maybe 's'just the medicine talking... are you naked...? ...Oh, we're all naked...!
"In the Line of Doody".
"Stick. Your option is a stick."
"I pulled the wrong one, alright?!"
Any of Skipper's over-the-top rants about Hoboken Zoo.
"I've rotted away in gulags, work camps, penal colonies in every dirty flea-bitten corner of creation. But I promised myself that I would NEVER end up in Hoboken! At least not alive! I will pluck out my own eyeballs! I swear to you!"
At the end of "Fit to Print," the penguins are convinced that their cover has been blown, and Skipper begins to assign new identities for when they need to flee New York. He tells Rico to pose as a professor of advanced linguistics at the University of Chicago. Cue 10 glorious seconds of Rico shouting, wailing, and making general random noises.
Juilien in Operation Antarctica is a walking parody machine. Most notably...
Also his acting of a Handsome Vampire. Listen especially close after the "deploy handsome vampire!" part!
"I am a handsome vampire. Bluh."
The entirety of "I Was A Penguin Zombie."
Kowalski: We'll honor him the way he would've wanted. By soldiering on like men. (cut to Kowalski, Private, and Rico crying like babies)
Kowalski: Okay, based on what I know about zombies, the safest place to hide is in a small dark room with creepy light. (Skipper jumps up against the window and Private, Kowalski and Rico scream)EVERYTHING I'VE LEARNED IS A LIE!
(later when the penguins have cornered Skipper on a roof)
Kowalski: No mercy this time, boys! Rico, see if you can shut off your conscience and sense of decency.
In Brain Drain, I can only grin madly at this little exchange which just adds to how absolutely adorably naive Private is. It's the tiniest of moments, which only makes it all the better:
Private: Gosh Kowalski, I think your brain power's already tip-top!
Kowalski: You also think there's a little man in the fridge who turns the light on and off.
Private: And one day I'll catch him!
Add into that Kowalski's dry voice, and the slightly exasperated expression on Skipper's face, and you've got yourself a "Wait, what did he just say?" moment.
Kowalski: I like peanuts and I like butter, but I do not like peanut butter. WEIRD!
From The All-nighter before Christmas, Elephant rules.
The storyboards are much funnier than the animation itself, such as Sunil Hall's drawings for "Time Out".
An illustration by David Knott of the main four in Boyne, Michigan; Skipper declares, "All right, boys, this is too cold, even for us! Time to implement, Operation: Fly South!", while Private and Kowalski clutch each other tightly (Kowalski's expression is partly what makes this funny), and Rico is lighting a bomb for warmth!
The scene in the penguins' quarters in "The Big Move" after the lemurs and all the small animals have left. Skipper is perfectly happy to be left in peace. I just love Skipper's stubbornness in that scene, the build up to We Want Our Jerk Back, and Kowalski's expression when Skipper talks into his ear.
Kowalski, Private and Rico are lying wide awake in their bunks.