Cars: Lightning McQueen, Strip "The King" Weathers, Flo, Ramone, and Tow Mater (sanguine), Chick Hicks (choleric), Sheriff (choleric), Doc Hudson (melancholic/choleric), Red (melancholic), Sally Carrera (phlegmatic), and Mack (leukine).
Finding Nemo: Nemo (sanguine/choleric), Marlin (melancholic) and Dory (phlegmatic).
The Parr family from The Incredibles: Bob (melancholic), Helen (choleric), Violet (phlegmatic), and Dash (sanguine).
Monsters, Inc.: Sulley (phlegmatic), Mike (sanguine), Celia (choleric), Fungus (melancholic), Boo (sanguine), George (phlegmatic/sanguine), and Roz (leukine).
Ratatouille: Linguini (sanguine), Collette (choleric), Anton Ego (melancholic) and Remy (phlegmatic).
Toy Story franchise: Woody (melancholic), Buzz (phlegmatic), Jessie (sanguine) and Lotso (choleric).
Up: Carl Fredricksen (melancholic), Ellie (choleric), Russell (sanguine), Kevin (phlegmatic), and Dug (sanguine/phlegmatic).
The Croods: Gran (choleric), Grug (melancholic/choleric), Eep (melancholic), Ugga (phlegmatic), Thunk (sanguine/phlegmatic), Sandy (sanguine), and Guy (leukine).
How to Train Your Dragon: Hiccup (melancholic/phlegmatic), Astrid (sanguine), Stoick the Vast (choleric) and Toothless (leukine).
Other characters: Mrs. Grunion (choleric/melancholic), King Tut (sanguine), Agamemnon (leukine), Leonardo Da Vinci (phlegmatic).
The Penguins of Madagascar's temperaments are more detailed than in the series: Skipper (choleric/melancholic), Kowalski (melancholic/phlegmatic), Private (phlegmatic/sanguine) and Rico (sanguine/choleric).
The original Guardians lineup, nicknamed "the Big Four" by Pitch: North (sanguine), Bunnymund (choleric), Toothiana (melancholic), and Sandy (phlegmatic).
Other magical characters: Jack Frost (sanguine), Pitch Black (choleric), and The Man In The Moon (melancholic).
The kids: Sophie Bennett (sanguine), Cupcake (choleric), and Jamie Bennett (phlegmatic).
The Shrek franchise: Shrek (choleric), Princess Fiona (melancholic), Puss in boots (phlegmatic), Donkey (sanguine), and Gingerbread Man (leukine).
Despicable Me, though it isn't until the sequel that the ensemble is completed: Gru (choleric/melancholic), Margo (melancholic), Edith (choleric), Agnes (phlegmatic), the Minions (leukine), and Lucy (sanguine).
The Herd in the Ice Age movies: Skrat (leukine), Manny (melancholic), Sid (sanguine), Diego (choleric), and Ellie (phlegmatic, with some sanguine aspects in the beginning).
The Last Unicorn: The Unicorn/Amalthea (melancholic), Schmendrick (sanguine), Molly Grue (choleric), Prince Lir (phlegmatic).
The main heroes in The Lego Movie: Emmet (phlegmatic), Wyldstyle —a.k.a. Lucy— (melancholic), Batman (choleric), Unikitty (sanguine), Metalbeard (choletic/melancholic), Benny (phlegmatic/sanguine), and Vitruvius (leukine).
Film — Live-Action
Non-Character Examples (mood and setting)
The four Alien films, not just in terms of their respectives tones but in light of their respective directors and the directors' general filmmaking styles: Alien is phlegmatic, Aliens is choleric, Alien│ is melancholic and Alien: Resurrection is sanguine.
The Avengers: The five male members and even their most distinctive colors fit a Combo Ensemble: Tony Stark/Iron Man (choleric gold + sanguine red), Clint Barton/Hawkeye (choleric/melancholic black), Bruce Banner (melancholic/phlegmatic, but green only when he turns Hulk, ironically); Steve Rogers/Captain America (phlegmatic blue + sanguine red), and Thor (leukine, neutral grey/black).
The S.H.I.E.L.D. agents: Phil Coulson (phlegmatic), Nick Fury and Natasha Romanoff (choleric), Maria Hill (melancholic), and Clint Barton (leukine).
A villainous example: The pragmatic Emma Frost (choleric), the somber and competent Azazel (melancholic), and the quiet, silent Riptide (phlegmatic), and The Faux Affably Evil Sebastian Shaw (sanguine).
Films in general
12 Angry Men: Of the 12 jurors, there are some that are purely one temperament.
Sanguine: Juror #1 (football coach; the foreman as well as one of the jurors; a helpful, likeable guy)
Choleric: Juror #3 (businessman; an old-fashioned, unmerciful, and extremely opinionated father)
Melancholic: Juror #2 (bank teller; a timid, go-with-the-majority type of guy), Juror #4 (stock broker; a very factual, unflappable, analytical perfectionist)
Phlegmatic: Juror #8 (architect —Mr. Davis—; first to vote "not guilty"; a strong-willed but compassionate and kind middle-aged man)
Leukine: Juror #6 (painter; a tough but very respectful hard worker)
But there are also some with blended temperaments.
Sanguine/Choleric: Juror #7 (salesman; an aloof, impulsive baseball fan)
Sanguine/Phlegmatic: Juror #12 (advertising executive; an easygoing, witty, and indecisive young man)
Phlegmatic/Melancholic: Juror #5 (ambulance crewman; a soft-spoken 30-something man of Jewish decent who immediately relates to the teenager's "slum" background; one of the first jurors to vote "not guilty"), Juror #9 (preacher —Mr. McCardle—; a wise, observant elderly gentleman), and Juror #11 (watch maker; a European immigrant who is very polite and deeply respects the American judicial system)
Alice in Wonderland (2010): Alice, Mad Hatter and the Gryphon (sanguine/choleric); Mock Turtle (sanguine); March Hare and Knave of Hearts (choleric); Dodo and Red Queen (choleric/melancholic); Pigeon (melancholic); White Queen and White Rabbit (phlegmatic/melancholic); and Dormouse and Cheshire Cat (leukine).
The Italian movie Amici Miei and its sequels: Mascetti (choleric), Necchi (melancholic), Melandri (sanguine), Sassaroli (phlegmatic), and Perozzi (leukine).
The four astronauts of Apollo 13: Fred Haise (sanguine), Jim Lovell (choleric), Ken Mattingly (melancholic), and Jack Swigert (phlegmatic).
The wives and girlfriend of the astronauts: Mary Haise (melancholic), Marilyn Lovell (phlegmatic), and Tracey (Jack's girlfriend; sanguine).
The Lovell family: Jim and Barbara (choleric), Marilyn and Jay (phlegmatic), Susan and Blanche (sanguine), and Jeffrey (melancholic).
Baby's Day Out: Eddie (choleric), Norby (sanguine), and Veeko (phlegmatic).
The Back to the Future film franchise: Marty McFly, Lorraine McFly, Doc Brown, and Goldie Wilson (sanguine) George McFly and Jennifer Parker (phlegmatic), Biff Tannen (choleric), Clara Clayton (melancholic), and Mr. Strickland (choleric/melancholic).
The McFly family: Lorraine (sanguine/choleric), George (phlegmatic/melancholic), Dave (choleric), Linda (melancholic), and Marty (sanguine).
The Big Lebowski: kind of. The Dude is the epitome of phlegmatic, as is Walter to choleric and Jesus Quintana to sanguine; Donny is arguably either phlegmatic or melancholic.
Braveheart: William Wallace (choleric), Campbell the Elder (melancholic), Hamish Campbell (leukine), and Stephen the Irishman (sanguine).
The Breakfast Club: John Bender (sanguine), Claire Standish (choleric), Brian Johnson (melancholic), Andrew Clark (phlegmatic), and Allison Reynolds (leukine).
The Charlie's Angels movies: Natalie (sanguine), Dylan (choleric), Alex (melancholic), and Bosley (phlegmatic).
Courageous: The four main cops: Adam Mitchell (choleric), Shane Fuller (choleric), Nathan Hayes (melancholic), and David Thomson (phlegmatic), plus their non-cop friend Javier Martinez (sanguine).
The villains of The Dark Knight Saga: Henri Ducard aka Ra's Al Ghul & Miranda Tate aka Talia Al Ghul (melancholic), Jonathan Crane aka The Scarecrow (phlegmatic), Harvey Dent aka Two Face (after his Face-Heel Turn), Bane, & Selina Kyle aka Catwoman (before her Heel-Face Turn)(choleric), and The Joker (sanguine).
The prominent heroic characters of the trilogy can also count: Bruce Wayne aka Batman (melancholic), Alfred Pennyworth & Lucius Fox (phlegmatic), Harvey Dent (before he becomes Two Face), John Blake, & Selina Kyle aka Catwoman (after her Heel-Face Turn) (choleric), and James Gordon & Rachel Dawes (leukine).
Diary of a Wimpy Kid: Frank Heffley (sanguine/choleric), Rodrick (choleric), Greg (melancholic), Susan (phlegmatic), and Rowley (sanguine).
The villains of the Die Hard films: Hans and Simon Gruber (choleric), Col. Stuart (melancholic), and Thomas Gabriel (phlegmatic).
Elf : Buddy (sanguine), Walter Hobbs (choleric), Michael Hobbs (melancholic), Jovie (phlegmatic).
Enchanted: Giselle (sanguine), Edward (choleric), Robert (melancholic), Nancy (phlegmatic).
Facing the Giants: The 3 main football players; Matt Prater (choleric/melancholic), Brock Kelly (sanguine/choleric), and David Childers (phlegmatic/melancholic). The 3 coaches/coach assistants: Grant Taylor (choleric/melancholic), Brady Owens (melancholic/phlegmatic), and JT Hawkins Jr. (sanguine/phlegmatic).
The Faculty: Delilah (choleric), Casey and Stokely (melancholic), Zeke (phlegmatic), Stan (sanguine), and Marybeth (leukine until she reveals herself to be the Queen alien).
Fireproof: The five firemen, Combo Ensemble. Caleb Holt (choleric/melancholic), Eric Harmon (melancholic/phlegmatic), Michael Simmons (phlegmatic/sanguine), Wayne Floyd (sanguine/choleric), and Terrell Sanders (leukine).
The coworker women: Deidra Harris (choleric), Catherine Holt (melancholic), Robin Cates (phlegmatic), Latasha Brown (sanguine), and Ashley Phillips (leukine).
Flintstones: Fred Flintstone (choleric/sanguine), Wilma Flintstone (phlegmatic/melancholic), Barney Rubble (sanguine/phlegmatic), and Betty Rubble (melancholic/choleric).
The Villains: Captain Barbossa (before his heel-face turn) and Davy Jones (choleric), Lord Cutler Beckett (melancholic), Blackbeard (phlegmatic).
Pulp Fiction: Jules (choleric), Vincent (melancholic), Butch (phlegmatic), and Mia (sanguine).
Reservoir Dogs: Mr. Pink (choleric), Mr. Blonde (melancholic), Mr. Orange (phlegmatic), and Mr. White (sanguine).
The Room is a good example of the four central characters manifesting all of the negative aspects of their respective humours, and none of the positive ones: Lisa (choleric), Johnny (melancholic), Mark (phlegmatic), and Denny (sanguine).
Singin' In the Rain: Cosmo Brown (sanguine), Don Lockwood (melancholic), Lina Lamont (choleric), and Kathy Selden (phlegmatic).
The adults: Maria von Trapp (sanguine/choleric), Captain Georg von Trapp (choleric/melancholic), Baroness Elsa von Schraeder (melancholic), Max Detweiiler (sanguine).
The children: Lisel and Louisa (sanguine), Friedrich and Brigitta (melancholic), Kurt (choleric), Marta and Gretl (phlegmatic).
The nuns: Mother Abbess and Sister Sophia (phlegmatic), Sister Margaretta (melancholic), and Sister Berthe (choleric).
Other: Rolfe (sanguine/choleric), and Herr (melancholic).
Stand by Me: Chris Chambers (choleric), Gordie LaChance (melancholic), Teddy Duchamp (sanguine, although he mostly displays the more negative traits, such as impulsiveness, self-indulgence and being a space case), Vern Tessio (phlegmatic).
A case could be made for this in the new Star Trek movie:
Sanguine: Kirk, the fun-loving fly-by-the-seat-of-his-pants tactical genius.
Choleric: "Bones" McCoy, the grumpy-but-compassionate healer.
Melancholic: Uhura, the perfectionist omniglot with a nurturing side.
The film version of Starsky & Hutch switched the personalities from the TV show to fit into the typecasts of Ben Stiller and Owen Wilson: Starsky (Stiller, melancholic), Dobey (choleric), Hutch (Wilson, sanguine) and Huggy Bear (phlegmatic)
The three generations of men from the Sonnenschein family played by Ralph Fiennes in Sunshine: The narrator's grandfather Ignatz (choleric/melancholic), the father Adam who pretends to be sanguine (is really melancholic), and Ivan himself (phlegmatic).
Super 8: Charles (choleric), Martin (melancholic), Joe (leukine), Cary (sanguine), and Preston (phlegmatic).