- Accidental Nightmare Fuel: Ed's mouth flaps sometimes cross into this territory, due to the movement not quite matching up with the speech.
- Bizarro Episode: The episode "Mako" revolves around an animated alien who comes to earth and takes over the bodies of whoever it goes inside. Its victims have no memory of what happens when the alien takes them over.
- Ear Worm: The theme song is the reason most people remember this show.
- Epileptic Trees: It's possible to interpret Mister Ed as the deranged hallucinations of a man who owned a completely normal horse, but in-universe evidence that Ed can talk is much stronger.
- Funny Moments:
- In one episode, Wilbur takes up painting and hires a gorgeous model as a bid to get Carol's attention. He brings her into the barn, where she announces that she needs to change. Wilbur modestly closes Ed's stall door...while Ed strenuously tries to keep it open.
- Hilarious in Hindsight:
- If you watch the series carefully, you'll notice that Alan Young is quite skilled at doing foreign accents. Guess who he had to do a Scottish accent for many years later?
- One episode has him playing the bagpipes and mentioning that he's Scottish.
- It's even more hilarious when he calls a character "Mr. Scrooge" in a Christmas-themed episode.
- Not to mention the fact that Alan Young had to do a Scottish accent when he played Wilbur's father.
- Retroactive Recognition:
- Alan Young as Wilbur Post.
- Long before he played Mr. Addison, Larry Keating previously played Harry Morton on The Burns and Allen Show.
- In addition to their work on Mister Ed, theme song composers Jay Livingston and Ray Evans also composed the theme song to Bonanza as well as the pop songs "Buttons and Bows", "Mona Lisa", "Tammy", "Silver Bells", and "Que Sera, Sera (Whatever Will Be, Will Be)".
- Ben Starr wrote 40 episodes. Starr is best known for co-creating Silver Spoons and co-developing The Facts of Life.
- Tear Jerker: An episode featuring Special Guest Zsa Zsa Gabor saw Gabor (playing herself) come to town to film a movie. The studio borrows Ed as a horse for Zsa Zsa to ride in the film; when she falls in love with Ed and refuses to ride any other horse, the movie studio offers Wilbur an enormous sum of money to buy Ed outright. Wilbur initially balks but Ed, realising how much the money would do for Wilbur, pretends he is star-struck and demands to be allowed to leave. Wilbur accedes and helps Ed pack up his things with tears streaming down his face as he does so.
- They Copied It, So It Sucks!: Some viewers found this a blatant ripoff of several films in which Donald O'Connor had adventures with "Francis the Talking Mule."
- Took a Level in Jerkass: Mister Ed becomes more demanding and more inclined to get Wilbur into trouble as the series continues on.
- Urban Legend of Zelda:
- Snopes.com, for a joke, posted a page that Ed was really played by a female zebra named Amelia.
- A popular rumour (referenced in the movie Dr. Dolittle), which may or may not have been started by Alan Young, was that in order to make Ed "talk" the production crew smeared peanut butter on the horse's gums. In actuality, the effect was initially pulled off by pulling on a nylon thread in the horse's mouth; however, eventually the horse learned to flap its lips on its own when someone touched its hoof. The horse soon figured out to simply wait until Young stopped talking before "talking" back, with no further cues necessary.
YMMV / Mister Ed