In "Parker Lewis Can't Win", Ms. Musso tells Parker not to try anything sneaky with the video he agreed to make for her, including "lip-syncing my homecoming speech to Mister Ed," to which he replies "Of course, of course."
In "When Jerry Met Shelly", Shelly says that she couldn't sit through Mister Ed is her life depended on it.
In the Dinosaurs episode, "Hurling Day", the show was spoofed as "Mister Ugh", with the title character being a talking caveman owned by a dinosaur named Woodrow. In a promo for the show, Mister Ugh is advised not to open a very important package addressed to his owner.
In his list of "Top 11 Saddest Nostalgic Moments", Critic imitates a depressed Mister Ed when going over the scene in The Never Ending Story when Artax drowns in the Swamp of Sadness.
In his review of the five-part DuckTales pilot, Critic notes that Scrooge is voiced by Alan Young and wonders if Scrooge made his fortune by betting on Mister Ed at the races and turning him into glue.
In "Rise of the Commercials", when Critic reviews various commercials for Teenage Mutant Ninja Turtles action figures, he says that the animatronics for the Turtles went from Jim Henson puppetry to dead-eyed corpses with fishing line-pulled lips like Mister Ed.