The themes for Birkin's various forms are often considered some of the most frightening music in the series.
Experiment room, anyone? It doesn't help that it's filled with three Lickers, gruesome failed experiments, dead Umbrella soldiers that look like HUNK, and one lone, empty capsule that's been broken open... from the inside. The incredibly unsettling music does not help.
The introduction of the Licker. When you enter that room in the police station and whatthefuckwasthat you glimpse a skinless, man-sized thing padding across the outside of the window like a gecko. You look back and... nothing there. Your paranoia approximately doubles, ready for you to meet it for real a tad later.
We do not talk about G.
Too bad, seeing as this is a Nightmare Fuel page and the G-Virus doesn't create zombies - it creates the unholy spawn of Satan◊ and some◊ kind of◊ devastating◊ cancer◊ - trying to kill the damn thing is preposterously risky, as the G-Virus was meant to have regenerative properties and every one of those is stages of the same mutations brought on by fighting it without eliminating it. You'll notice that that second one there? His head has started deteriorating and being replaced with a new one - that's his face you'll notice on the left side of his chest if you look close enough. That new head, as seen in the third stage, looks like a freaking devilish skull, and starting there, it starts developing multiple limbs, all tipped with foot-long sickle claws, and More Teeth than the Osmond Family until it finally hits the last form, a shapeless toothy blob of flesh with corpselike arms sticking out all over it. It also creates embryos it launches into live humans' mouths once it gets an opening, which then develop inside said humans until they're finally developed and bursts out of their chests and rapidly grow into something like this◊ (or, more famously, this◊). It just gets worse and worse when you hear its theme music. Or, for that matter, if you remember that, like most of the main boss monsters, it Was Once a Man (albeit a truly vile man), an Umbrella scientist with a fellow Mad Scientist for a wife and an Ill Girl kid, both met through the game in which it appears, who injected it into himself both in an attempt to save himself (again, its regenerative properties) after surviving a machine gun spray and to keep Umbrella getting ahold of this thought-to-be masterpiece of his (it in itself was obviously not as planned). Instead he ends up turning into a mindless abomination against all that is holy with nothing on its mind but planting chest-bursting embryos inside living targets and devouring corpses... though before his conscience completely degenerates, he does have enough connection to his former self to scream out his daughter's name a few times, which she misinterprets as her daddy being attacked by the monster lurking in the sewers, that monster little does she know actually being... you get the picture. Yeesh.
Hearing G's roar for the first time is utterly chilling, especially since it comes out of nowhere.
Sherry: But there's something out there. I don't know what it is, but I saw it. Much larger than any of those zombies... and it's coming after me!
(loud, inhuman roar suddenly cuts through the air)
Arguably, its actually not all that inhuman. It just sounds like a person making a "RAAAR!" sound. Which makes it so much worse, rather than the Narm you might think it would be.
Police Chief Brian Irons was horrifying. He raped at least one woman in college, he may have had his secretary killed, arrests a reporter without charging him, and his policies towards ammunition storage and the door key system were intentionally designed to cause as many casualties as possible in a disaster. It's bad enough that he moved through the police station, killing off survivors so that they couldn't leave his "beautiful city," (and then later wrote a diary entry about how beautiful the pained face of one of his employees was) but his love of taxidermy and stuffing the mayor's daughter as a trophy to "preserve her beauty"?! Just goes to show you don't have to be infected to be a monster.
The novelization implies that the "stuffing" wasn't just in the taxidermy sense...and that it was done after she was killed.
This one's a bit of a stretch and a minor example,but one of the files you pick up, named "Secretary's Diary B", Has Brian Irons's secretary saying that she "found out what the chief has been hiding all along" and that if he finds out, her life is going to be in serious danger and the next page says it got late and that she's just going to take that day at a time... and then we turn to the next page. The last page is ominously blank. Since every other diary up to this point has been written in to the very last page, we can deduce that Irons got her before she could finish writing... Now you see, this wouldn't be that scary if this wasn't freakin' BRIAN IRONS we were talking about.
The only music in that game that wasn't unGodly scary was the finale music, which was actually pretty epic.
Three words and a hyphen: Two-Way Mirror.
Walk into a room, totally quiet and completely free of enemies, but with that damn two-way mirror that you can't help watching. Then, when you grab one specific item in the room, a licker crashes through the mirror and attacks! Even if you know it's coming, it doesn't help.
Two words: Mr. X. AKA Tyrant T-103. Its theme music alone will terrify you, and there's the paranoia that it may smash its way through the wall any moment.
Which he does at one point. And unlike Nemesis, there is no forewarning to his appearance, but when you hear the characteristic Scare Chord, you know its time to haul ass.
The blood-sucking, poison-spraying, giant moth, and its lair, the eggs on the walls and ceiling with maggots dropping out of them, and the music.
Files you find in the police station are mostly from police officers who survived the initial zombie attack. They talk about plans to take the station back and rescue survivors, but all of it failed. The most disturbing one is from an officer named David. He talks about how he ran as a fellow officer who saved his life was killed, screaming for help and how hopeless and insane the situation is. He ends the letter mentioning how his old friend Mossberg is going to be "turning one more body into fertilizer".
The scene where Marvin (the lone officer you meet in the police station) becomes a zombie. You find him just in time for him to transform, and you get a close up of his face decaying for your pleasure.
Lickers. Flayed creatures that can crawl on the walls and ceiling and attack you with their claws and absurdly long tongue. But what seals the deal is how they are introduced. The first glimpse you get of one is as it zips by a window. Then, you enter a hallway that is drenched in blood. A corpse lays in the hallway, decapitated with its head twisted off. All while the sound of something like a leaky faucet is heard. You get a short cutscene of your character looking up to see the Licker, slowly stalking along the ceiling, hissing and dropping to the floor in front of you. Is it any wonder they have become one of the series' most iconic enemies?