Funny: The Binding of Isaac

  • This, of all games, has one where in one of the endings, Isaac picks up a fetus in a jar...and is shown wearing a Dr Fetus Costume.
  • Unlocking a treasure chest, only to find another treasure chest inside it. It appears Edmund likes to toy with expectations.
    • Or unlocking a treasure chest, finding another treasure chest in it, and then finding another treasure chest in that.
  • Number One. The look on Isaac's face as he chooses to pee on everything is just priceless.
    • Even better, beating Mega Satan with this power up. Enjoy killing Mega Satan by peeing onto his face! Doubles as a Crowning Moment of Awesome, obviously.
  • Isaac can wear his mother's brassiere or her menstrual pad as items. Doing so causes the enemies to freeze or back away, respectively. Presumably, they're Squicked out by the sight of it!
    • Segmented enemies like Larry Jr. will even fall apart when they try to flee. They are so disgusted that it breaks the laws of physics.
  • When you meet Greed in a hidden room, you can immediately leave through the hole you made without having to defeat him. Hi, Greed. Bye, Greed. If you have X-ray glasses, you can even use his room as a shortcut, running past him without ever engaging him.
    • Sadly no longer works in Rebirth, since the entrances to the rooms around the hidden room get blocked until you defeat him.
  • The beggar that runs the shell game in the arcade is a jerk. Giving you a giant grin as he takes all your money without giving you the items you want? Seeing the slot machine throw a troll bomb at him is just too hilarious a karma to be true.
    • There are times where beggars will unveil a troll bomb right next to themselves.
    • Similarly, sometimes the Beggar "rewards" you with a troll bomb. His eagerness to kill himself makes whatever money you wasted on him worth it.
  • In Wrath of the Lamb, getting the Speed Ball item (a syringe with white fluid in it) gives Isaac very large, dilated eyes and a little smile. It looks incredibly goofy (though less so once you see the bloody syringe sticking out the back of his head).
  • The glitches in this game can sometimes cause hilarious reactions from players. Especially since, given the tone of the game and previous games by Edmund Mcmillen, it's difficult to tell if some of them are actually glitches.
  • One of the random effects of pills is "I Found Pills" with the description "...and ate them." This pill also causes Isaac to get a silly, derped look on his face and make a faint "duuur" noise when you take it.
  • Another effect of the pills is the "Puberty" pill, which causes Isaac to grow a few scraggly hairs on the top of his head. Then you realize, those are pubes growing on his head...
  • Even more amusing pills in the remake. A new pill effect is "R U A Wizard?" This causes Isaac to get a derpy,wall eyed look that forces him to shoot his tears diagonally for a short while.
    • The fact that the game treats this as a positive pill effect makes it even funnier.
  • Any of the secret messages from the Fortune Telling machine. Some of these include cryptic nonsense like "LOOK TO LA LUNA" or "DON'T LEAVE THE HOUSE TODAY". Then there are some that seem to be talking to the player, such as "GO OUTSIDE" or "YOU ARE THROWING YOUR LIFE AWAY". The way the game is just totally fucking with you in this moment is priceless. Oh, the best of all these? YOU ARE WORSHIPPING A SUN GOD.
  • Some of the boss enemies make some pretty funny expressions, such as Chub's poker face during her intro, War's Oh, Crap face when he runs out of stamina, Pin's stereotypical frown (it looks exactly like :c), or even Loki's expressions of pure glee while he fires his 4 way shots.
  • The fact that of all the Seven Deadly Sins, the only one without a unique sprite is Sloth. Or, in more modern terms, laziness.
  • One of the between-level dreams features Isaac using the toilet...and discovering there's no toilet paper left. His reaction is the typical "No!" reaction used in other dreams, but it seems more like he's going "Curses!" this time around. Don't worry, Isaac, other people can relate.
  • Fighting Wrath, a mini-boss who uses bombs...while you have the Remote Detonator on hand.
  • The fact that you can sell your soul... for a quarter.
    • In Rebirth, picking up the Rosary item allows you to potentially sell your soul for the Bible.
  • Rebirth adds an item called "How to Jump", its description being "it's time you learned." The animation for jumping is just Isaac's head slapped onto the hopper sprite. It's as ridiculous as it sounds; it must be seen for oneself.
  • In Rebirth, the "A Card Against Humanity" card. Its effect is so ridiculous, that you can't help but to laugh at it. What does it do? It fills each empty space on the room with poop! Try using it in a big room or in a boss fight for added hilarity.
  • One secret seed you can input, BASE MENT gives you a seemingly normal run. Basement I and Basement II are normal, but instead of moving on to Caves I, you go down to Basement III?, which is exactly like Basement I. And then Basement IV?! and Basement V...And then Basement VI and Basement VII. If you have time, you can play the seed for 4 hours!
  • Why does Judas wear a fez? It's a reference to the Fez creator, Phil Fish, who Edmund Mc Millen saw as a traitor. Clever, and funny.
  • The red poop dropped by the Carrion Queen will damage any other enemy that touches it. Because of this, during the Boss Rush, it's possible for some bosses to die as soon as they enter the room.
  • Cancer, in real life, is a horrible, life-threatening disease. Cancer in The Binding of Isaac is probably one of the best trinkets in the game, since it doubles your tear-firing rate, even if you've already hit the cap. The tagline description for it in Rebirth is even "Yay, cancer!"
  • One ending (ending 10 in ''Rebirth) has Isaac trying to open up the chest only for It Lives! to appear, try to growl intimidatingly, and give an incredibly cheeky grin.
    • The very next ending has ??? give Issac a cheeky grin as well. Made even funnier in the original game/WotL, where the Dramatic Chipmunk Sting plays during this scene.
  • Several combinations of items can lead to either Made of Win or Epic Fail situations. Sad Bombs (or Tammy's Head) plus Rubber Cement plus Ipecac equals utter carnage as you try to survive from your own explosive tears. Brimstone (or playing as Azrael) and Anti-Gravity leads to lines of charged beams.
  • There's a bonus song on the soundtrack called "He's the Number One." And it's so bizarre it's hilarious.
    He's the number one. God's favorite son. His flowing robes reach down from heaven. His hands will touch you! Build a world around you! The light within his heart is enough to heal us all! He will fill your soul when life takes its toll. Your only goal is to find his kingdom. His love is real! The golden seal! Trust in the book and the bread you took!
    "Once I though I had it good, probably mom misunderstood, when god came down from heaven, and turned the shit up to eleven."
  • In order to accommodate Nintendo's policies, Rebirth will be somewhat censored, including a fig leaf to cover nakedness and God being replaced with a dog. The censoring is an April Fool's joke, but the game will be released on the Wii U, New 3DS, and Xbox One.