- Acting for Two: The second mission in both versions puts the Create-A-Bots against each other, and they are both Steve Blum.
- The Other Darrin: Most of the film's voice cast do not also play their roles here.
- Mark Ryan as Ironhide (replacing Jess Harnell). Ryan auditioned to play Ironhide in the film, and his presence as Ironhide here suggests he did have the part at some point.
- Fred Tatasciore as Ratchet (replacing Robert Foxworth)
- Andrew Kishino as Jazz (replacing Darius McCrary)
- Daniel Ross as Starscream (replacing Charlie Adler)
- Keith David as Barricade (replacing Jess Harnell)
- Frank Welker as Megatron crosses into The Original Darrin territory, since he was passed over in the film for Hugo Weaving, but gets to reprise his role here.
- Role Reprise: Peter Cullen as Optimus Prime.
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