It can be hard, a lot of the time, to figure out just where Survival of the Fittest is on the overall scale, but it appears to be more towards the cynical side due to the downer nature of the endings for the most part and the fact that fate itself seems to crack down on optimistic characters. Hero types usually get themselves killed, and even if they don't die right away they usually see all their friends die first. All escape attempts are brutally crushed, and even hacking the system and putting in a powerful computer virus that basically destroys all SOTF systems from the inside doesn't work until it's too late for anything to be done. By v2, all damage has been fixed, and the systems have been changed so they're literally impenetrable.
In a strange bout of irony, the same virus comes back to bite the terrorists in the arse in V3 purely by accident, even worse than before.
Encyclopedia Dramatica is what happens when the most cynical side of the internet creates a wiki.
Sailor Nothing occupies an odd place — it's far grimmer than its inspiration Sailor Moon, but ultimately has a core of idealism. The Power of Friendship plays an important role in keeping the heroine together, and the key to defeating the Big Bad ultimately turns out to be forgiveness.
Chaos Fighters is extremely idealistic that everyone fighting at the good side is invincible heroes, having love interests and their mission is guaranteed a success no matter how hard it is. They can even destroy armies of demons or bandits by themselves.
Red vs. Blue has a tendency to be rather cynical, considering how virtually everyone in Blood Gulch dislikes one another.
Revelation ends on a surprisingly idealistic note. Sarge has finally learned to respect the Blues, and organizes them and the Reds into a Five-Man Band to save Tex and Church and take down the Meta. Washington is saved by the Blues, and is recruited into their ranks. Church willingly pursues Tex inside the Epsilon unit, but being trapped there himself, he decides to relive Alpha's memories of Blood Gulch, deeming them pleasing memories.
Season 9, on the other hand, is much more cynical than Revelation. In the past timeline, virtually every Freelancer is out for themselves, as well as a poor team player in general. The UNSC-Insurrection war is full of Grey and Gray Morality, in that the Insurrection has legitimate beefs with the UNSC, and the Freelancers show almost no regard for civilian life. York somberly lampshades this by saying ("We're the good guys... right?") In the present timeline, Epsilon, actually experiencing Alpha's memories rather than simply remembering them, begins to develop his template's anger issues. When the Epsilon unit is about to fail, he realizes that the best way to be at peace is to let go of Tex's memory - and since this Tex is a memory, it amounts to killing her. And to top off the cake? The unit wasn't failing; the real Reds and Blues were breaking into the unit to rescue Epsilon, so he basically killed Tex for nothing!
Homestar Runner is set in a largely optimistic setting with a select few deeply cynical elements and characters. Strong Bad and The Cheat are always committing petty crimes, Bubs always swindles his customers, Coach Z has a thinly-veiled disturbing background, and the King of Town is largely friendless. Oh, and everybody dies in Teen Girl Squad.
The Most Popular Girls in School falls onto the middle part of the scale, with a tendency to bounce back and forth depending on the scenario and the characters in said scenario. Many people in this show are either jerks, idiotic, or both... however, there are enough good-hearted people with their idealistic ways to help balance out the cynical Jerkassersy. Its little brother show, Dr. Havoc's Diary, on the other hand, falls a bit further onto the Cynical, gritty side of things while still retaining the Gray and Grey Morality.
RWBY is very idealistic despite the nature of the setting: humanity is sequestered into tiny pockets of civilisation as demonic monsters have overrun the planet, but the situation isn't as hopeless as Attack on Titan and the presence of effective monster slayers called Huntsmen puts the story well into Lovecraft Lite territory. However, the events of Volume 3 have pushed the story quite far into the cynical end, as society has been thrown into chaos by a terrorist attack during a world fighting tournament in which Pyrrha and Penny have perished and the villains have made huge gains, and the titular team of heroines have all to varying degrees been betrayed, disgraced, hurt and placed into terrible situations with their innocence and idealism destroyed - Blake has disappeared without a trace, Weiss has been taken back to Atlas by her domineering and abusive father, Yang is a crippled, depressed empty shell of who she once was, and only Ruby is still fighting and seeking answers.
Ruby Quest is pretty dang high on the idealistic side for a Cosmic Horror Story about a medical facility that fell to madness and Body Horror when they tried to exploit a strange substance as a cure. And yet, despite the zombies attacking, anyone who's injured mutating and losing their minds, and Ace, the story also shows that the monsters can be defeated, Ruby and Tom really can trust each other, and people can be saved. By the end, Red has killed himself permanently, therefore exiting the Glen on his own terms, Bella is also permanently dead and thus out of her And I Must Scream situation, and Ruby and Tom have freed Jay, another sane prisoner, and all three have escaped the Metal Glen. The final panel shows that Daisy (who'd died early on as the Hound Zombie, a monster who attacked Ruby) has come Back from the Dead and now might have her own chance to escape the Glen.
Atop the Fourth Wall is interesting because its placement on the scale is all over the place. The very nature of the show is cynical due to it being "Where Bad Comics Burn", but on the other hand, Linkara has often stated that part of the purpose of the show is so to teach people how to make good comics by pointing out the bad parts of bad comics and why they don't work. In the greater story arcs, the universe Linkara lives in is full of Eldritch Abomination outer gods, Multiverseral Conqueror visitors, evil cults and the occasional Mad Scientist. And something does try to kill him every year. And yet he's also got a team of True Companions that will always back him up, stories of redemption and family, and in the end, triumph over adversity is a greater theme in his storylines more than failure is.