Jump Scare: Towards the end of the film, there's a moment when Duke and Riley, who are fighting, fall off the hay loft together. It's shot and edited in a very strange way, by showing the duo heading for the edge and then abruptly jump cutting to Riley moving towards the camera, followed by another jump cut of Duke doing the same thing. Both instances cause a startled "Aaah!" from Mike and the bots.
Self-Deprecation/Old Shame: In the Mirror Universe, the "Joey the Lemur" song and the Eight of Chris Lemmon are considered hilarious. The horrified reactions of our universe's Tom and Gypsy say it all.
Tom: There's no lemurs in this movie!
Separated by a Common Language: Servo and Crow both speak English, but are programmed with different regional slang terms. So the two are confused when they use different words for bubblers/drinking fountains, shows/movies, dinner/lunch, "come with"/"come with me," Coke/root beer, and "borrow me some money."
To A Christmas Carol: After Tom and Gypsy have returned from the mirror Universe, Crow expresses doubt that anything really happened. Tom quotes Scrooge's suggestion that it was the result of "Perhaps an underdone potato, or a bit of undigested beef."
Smart Ball: In the intro sequence Mike programs the Bots to have different regional speech patterns. He's not the tech guy like Joel and admits it's the only thing he knows.
In another example of the Mirror Universe at work, the usually-dim Gypsy is actually the first to realize what's gone wrong when she and Servo find themselves in Deep 13 with Evil!Mike and Evil!Crow. (Of course, she's not running the Satellite of Love at the moment, so...)