When Heinz released green ketchup a few years back, they advertised it with a series of people declaring "It's twisted and wrong!" While most people agreed, it somehow lasted six years before being mercifully discontinued.
Recently, Corn Nuts has been advertised as "Corn Gone Wrong!"
Used seriously in The Royal Tenenbaums. Margot tells Eli, with whom she's having an affair, that she doesn't love him; he replies, "I know, you're in love with Richie. Which is sick and gross." He has a bit of a point, since, adopted or not, Richie is her older brother.
From Fanboys: Eric and Linus are taking out their frustrations with each other in the form of an argument about whether or not Luke and Leia were attracted to each other before the Episode VI reveal. As Linus leaves he yells back, "They were siblings, you sick bastard!"
In Norm MacDonald film Dirty Work, Norm's character Mitch gets confronted by his best friend's father Pops, who says that he's also Mitch's father. When Mitch asks for proof, Pops pulls out a locket containing a picture of him on one side and Mitch's mother on the other. When Mitch says that doesn't prove anything, Pops pulls out pictures of him and Mitch's mom having sex. Take a guess about how Mitch reacted.
There's also a second usage later, when Mitch's best friend Sam finally learns that the two are half-brothers and they start reminiscing about old times. This goes on until they start talking about Mitch's childhood crush on Sam's sister, and the time that Mitch and the sister hooked up in high school. Cue a belated case of Fridge Horror for both of them and the two deciding to never bring this up again.
Vorkosigan Saga: Lois McMaster Bujold's novel Cetaganda depicts a planet where genetic engineering has become a fine art. When two visitors from a slightly more normal planet happen to walk by a kitten tree in fruit, one remarks, "Now that is just wrong." This trope was used tactically to end the Vorryuter subplot in A Civil Campaign. Richars (cousin of the late count), apparently taking things personally, hired thugs to emasculate Dono (sister-turned-brother of the late count) the night before the Council of Counts decided on who inherited the seat. Ivan Vorpatril, after helping incapacitate said assailants before serious damage was done, hauled them and Dono not to the local guard or hospital but to a meeting of Richars' main supporters. Oddly enough Richars lost most of his votes the next day.
In Christopher Moore's A Dirty Job, Charlie Ascher walks in on one of his employees and one of his former employees (the first a middle-aged retired and paranoid cop, the latter a twenty something goth girl who had worked there since her teens and is alost like a bratty younger sister to him) shagging in the thrift store he owns. This trope is his reaction.
In The Dresden Files, Molly -Harry's teenage apprentice- in a helpful attempt to charm some information out of someone takes off her bra and goes out to sweet talk the stooge. Harry and the injured warden, Morgan, watch her go, and Morgan makes a comment along the lines of 'nice'. Harry is shocked. Morgan says he's old, but he's not dead, and Harry is thoroughly sickened by the mental image. Made all the squickier for Harry by the fact that Molly isn't just his apprentice, she's his friend's daughter, whom he's known since she was ten.
Ciaphas Cain: In Traitor's Hand, Cain and some Imperials Guardsmen infiltrate a temple of Slaanesh and observe the murals inside:
One of the guardsmen: Is this even anatomically possible? Cain: No, it's not. And even if it were, it'd be against regulations.
In Hell To Pay, John Taylor gets bored waiting for a tycoon's attention, so uses this trope to clear the room of yes-men, switching the tycoon's Ominous Multiple Screens to the most Sick and Wrong pornographic program he knows of. The contents of the "Celebrity Perversion Hour" are enough to send the crowd of yes-men climbing out the windows to avoid it.
In one episode the naked ghost of an old man walked through (literally) a group of schoolgirls, causing the observing grim reaper to remark, "Oh, that's so not right for them."
Another episode of Dead Like Me (specifically, Season 1, Episode 11), Dolores is throwing a Mexican-themed going-away party for Millie, complete with margaritas, pinata, and a real live burro. George sees a man leading the burro into a room at the end of a hallway and says, "That's not right."
Because the writers were obviously very fond of this trope, it's also used in the final episode (Season 2, Episode 15). George and Mason are getting candy at various houses on Hallowe'en Night on the way to their reaps, and they stop at a house whose owner is dressed up as a sexy, corseted vampiress. The house turns out to be a whorehouse and the owner its madam; one of the prostitutes steps out for a smoke wearing peach-coloured lingerie, and tousles the hair of a trick-or-treating child, leading George to say, "Oh, that's just not right!"
In Frasier episode "Voyage of the Damned", Maris puts on a cheesy 1970's salsa-disco song called "Do the Barracuda", causing Frasier to react, "Oh, oh, that's bad. On so many levels."
Another episode finds the Crane brothers attempting to replace Martin's ratty old recliner with an expensive leather massage chair. Once they turn the massager on, Martin's response is, "That's disgusting!"
From Friends, when Phoebe's 18 year-old brother is going to marry his 44 year-old teacher:
Phoebe: I don't want to be all judgmental, you know, but this is sick; it's sick and wrong!
The Mythbusters did a Breaking Bad special, testing the effect of the show's Hollywood Acid scene. The Mythbusters test it with a headless pig, a raised bathtub, and an extremely potent variant of sulfuric acid. After the smoke cloud clears, the guys have a look at the results.
Adam: Augh! Jamie: That's just wrong. Adam: It smells terrible. Jamie: That's one of the ugliest things I've ever seen. Adam: It really is. *looks to the camera* It's one of the ugliest things he's ever seen. Think about that for a second.
Most people's reaction to FATAL. RealisCLOACATIME!
Warhammer 40,000 has plenty of this. Being a grim dark world, there is no shortage of Sick and Wrong things in it. A few examples would be the Forces of Chaos (Slaanesh and Nurgle in particular), the victims of Dark Eldar, The Imperium of Man, who have been described as Nazis in Space!, who are only "good" by comparison of MUCH worse horrors, and many more.
Phantasmagoria: A Puzzle Of Flesh: First (?) and most memorable utterance of the phrase 'Sick and Wrong' came from this 1996 puzzle game. It contained quite a few Squick sequences captured in video detail. The character saying the lines was a straitjacketed girl in an asylum (the story writer in a cameo, incidentally) who repeated the phrase in tones that alternated between accusative and exhausted. This was aired throughout the scene while the main character, strapped in his own straight-jacket and doped up in a wheelchair, had to figure out how to escape while witnessing and hearing things that were exactly as the girl described: "Sick and wrong!" As is the game.
There is an achievement in Cookie Clicker called "Just Wrong" achieved by selling a Grandma.
"I thought you loved me" — Flavor Text for the Just Wrong achievement
"News : cookie manufacturer downsizes, sells own grandmother!" — "News" feed report when Just Wrong achievement is unlocked.
In Ultra Fast Pony, "The Pet Games", Twilight Sparkle serves as a judge for the Games, which includes a talent show. One contestant's talent is racism. Twilight's reaction: "This whole thing is sick and depraved! But yes, he was the fastest."
Exterminatus Now: After Blasphemy the Chao transforms from "really cute" to "daemonic abomination, but still kind of cute on some level", it rips apart and devours the daemon it got its abomination-ness from. Even Lothar Hex winced and said "Ooh, that ain't right". This is a character who once sat bolt upright and shouted "You can't prove I ate that baby!"
Inhuman: Invoked word for word, in reference to the treatment Grey has received.
Polk Out: It's basically the punchline to 90% of the strips.
Scary Go Round: The VitRx potion that enlarged the younger Winters girl was first tested on animals:
The Spoony Experiment: Spoony invokes this after seeing a scene in the Ultimate Warrior's Christmas special comic depicting the Ultimate Warrior pulling on Santa Claus' pants while standing over a naked, unconscious, Santa Claus with a strange white substance on him. "He raped Santa!"
The podcast Sick and Wrong where, as the title implies, they discuss sick and wrong news from all over the world. It's NSFW.
On Kim Possible, Ron (and Barkin) would frequently describe squicky things as being "sick and wrong." Really weird stuff would elicit the response "This goes beyond sick and wrong! It's Wrongsick!''"
On Ed, Edd n Eddy, Edd's response to finding out Ed's parents completely removed the basement stairs solely for the purpose of keeping Ed grounded in "Three Squares and an Ed" is "That's... disturbing." He has a similar reaction in "High-Heeled Ed" to Ed's claim "Sarah likes to watch me eat yogurt from my belly button."
An episode of SpongeBob SquarePants had Spongebob and Pat see a hibernating Sandy. Spongebob states that they shouldn't disturb her sleeping. Patrick responds, "That's not disturbing. This is disturbing." Patrick promptly folds his back fat to form a face and says, "Hi. Sponge. Bob. My. Name. Is. Pat. Back." Spongebob replies cheerily, "Ha! That is really disturbing!"
Joozian #1: Yeah, suck my jagon! Joozian #2: Yeah! Now you suck on my jagon! Oh yeah!! Stick your finger in my thrusher! Oh yeah, suck it. Suck that jagon! Stan: Dude, I have no idea what we're seeing right now, but I have a feeling it's really, really wrong.
A short part of Robot Chicken's Star Wars sketch includes a bit with Luke and Leia lying in bed, obviously shortly after having sex. Luke looks pleased, while Leia just looks disturbed and finally says "That was so wrong".
On Phineas and Ferb, when the former realizes that they're in their sister's stomach, the latter's response is that its "wrong on so many levels". When Phineas then realises they're also technically on a date with Jeremy, Ferb repeats the line.
In the Regular Show episode "Muscle Woman". After Muscle Man and Woman passionately embrace each other and tongue kiss, Rigby says what everybody is thinking.
Rigby: This is the most disturbing day of my life.