- Author's Saving Throw: After defeating Phancero by accident instead of catching it, the chat found out that the entire Boss Rush preceding it had respawned. This immediately caused heavy backlash from players, as catching Phancero was a needed requirement for the run to end and beating every boss had taken close to an entire day. This especially angered those who had made major efforts to contribute during the battles, and saw themselves denied a rare run badge as a result because of some malicious inputs. The developers quickly fixed the situation by deactivating all of the battles save from the first one, admitting that this was a remnant of the run not initially requiring Pokédex completion.
- Crosses the Line Twice: "KKKB" is pretty much the worst and most offensive possible name that the Voices have ever named one of their Hosts, but that's precisely what makes it all the more hilarious. It helps that they have very little control over naming their characters to begin with, which makes the tasteless nature of the result more a result of misfortune than anything.
- Ending Fatigue: The last few days of the run felt like this to some players, especially those most eager to return to PBR, as the developers had to block access to Mt. Silver for several days in order to get the final challenge ready, which turned out to be an excruciatingly long Boss Rush against no less than 11 different opponents, some of them proving to be particularly difficult to defeat. Then they revealed that this was not the final battle, and threw three more conditions to end the run: completing the Pokédex (a whopping 400 species to collect, including one that required navigating through the entire Silver Cave again), completing every Trick House puzzle, and defeating the actual final boss, which was situated at the bottom of yet another puzzle-riddled dungeon.
- Memetic Mutation:
- Nightmare Fuel: On Day 3, VVVVVV, the Turtwig that was left in the Daycare, was revealed to have been turned into a blocky mess of pixels with an overly-long cry constituting of mostly silence followed by a few garbled-sounding notes, which caused all of the game's music to distort after being heard.
- Sidetracked by the Gold Saucer: The chat spent some time fumbling around before getting to the final battle, as they absolutely wanted to print their newly received Diploma, then absolutely wanted to put up their newly-received Gold Trophy in KKKB's room, the latter being a more daunting task than it should have been due to the peak in active viewers.
- Unexpected Character:
- Given that Chatot was the starter of Twitch Plays Pokémon Chatty Yellow, that it was the focus of the teaser poster for the run, and that the game itself both referenced its signature attack in its title and featured it prominently in its title screen and intro sequence, players were understandably very surprised when the Egg given to them at the start of the game hatched into an Unown instead.
- Few expected the fused Pokémon from Twitch Plays Pokémon Fused Crystal to actually appear as they were in the original run, but sure enough, Y appeared as the Gym Leader of Mahogany Town, using his original team of Megastoise, Quagynx, Togetape, Noctdrio, Jumpanium, and Steelurret.
- There were a lot of guesses as to who would be found on top of Mt. Silver; popular theories included ×ᴹɴ(? from Twitch Plays Pokémon Chatty Yellow, the original RED from Twitch Plays Pokémon Red, his namesake from Twitch Plays Pokemon Gauntlet Red, one of the Pokémon Professors, or even one of the developers themselves. Ironically, the one opponent that nobody expected was Red with his unmodified team of Pikachu, Espeon, Venusaur, Charizard, Blastoise, and Snorlax, taken straight from the original games.
YMMV / Twitch Plays Pokémon Chatty Crystal