- Voiced by: Robbie Daymond
- Adaptation Dye-Job: Peter is usually portrayed with having either brown, blue, or hazel eyes in the comics and other versions. Here, his eyes are green.
- Adaptational Badass:
- Peter's physical strength has gotten an upgrade from most incarnations where he's 15 years old. He's strong, but you wouldn't think he could catch a falling helicopter or a careening van as a teen, something he could do in his adult years. This version doesn't make that feat look hard at all. Then there's also the fact that he's strong and fast enough to create a miniature cyclone simply by swinging a heavy object around him him in a circle.
- Same goes for the enhancement provided by the Symbiote. Previous versions did enhance his strength, but not to the point his punch would generate a shockwave and cause an Elemental Shapeshifter to back down.
- Adorably Precocious Child: His interest in the sciences goes way back, to the point that he'd rather play with a chemistry set than go outside and hang out with other kids his age when he was younger.
- Adorkable: As with most versions, Peter is a science geek first and foremost, but this version plays it up even more. For instance, in the first short, he is visibly very excited about visiting Oscorp, and so overenthusiastic he ends up stealing the guide's lines twice, only to apologize each time.
- Alliterative Name: Peter Parker.
- Animal-Themed Superbeing: In case Spider-Man didn't tip it off for you.
- The Apprentice: Peter becomes one to Max Modell so that he can pay off his tuition, lasts until Season 2.
- Ascended Fanboy: A fan of Superheroes who became one himself.
- Badass Adorable: An adorkable fifteen-year-old science geek who fights supervillains.
- Badass Bookworm: He not only solved an incredibly complex equation that served as an admittance test to an exclusive science school, he repurposed it to help fight a fire that the Villain of the Week had caused.
- Beta Outfit: His original costume is a hoodie with goggles and a sweatpants similar to the original outfit in the Marvel Cinematic Universe.
- Berserk Button: "Spider-Man on Ice" reveals that Peter despises the idea that inventions could be weaponized, calling Harry out for turning their thermal gauntlet into a sword.
- Beware the Nice Ones: He's jovial, wise-cracking, and friendly most of the time. You really don't want him angry though.
- Big "NO!": Lets one out when Flint Marko AKA Sandman is seemingly killed by his daughter.
- Brainwashed and Crazy: He is made into an Unwitting Pawn of Doctor Octopus via mind control in "The Hobgoblin, Part 1". Thankfully, Harry is able to snap him out of it.
- Brainy Brunette: Brown-haired and a Teen Genius.
- Clothing Damage: His original costume gets continually ripped up as he's forced to repair it. He finally ditches it in favor of his high-tech version he made in his lab while facing the first Spider-Slayer.
- Comes Great Responsibility: Well, it is Spider-Man after all. Uncle Ben framed it as a pseudo-math equation in this continuity.
- Composite Character: He is a teenage version of the Dan Slott era Peter Parker, is a fan of the older heroes just like the MCU and ultimate cartoon Peter and he is the unwilling 6th member of the Sinister Six like in the Ultimate Comics.
- Cool Loser: As usual. Despite being chipper, friendly, funny, and knowledgeable, he's still so much of a nerd that Liz Allan rolls her eyes and sighs at the thought of being his tour chaperone.
- The Dark Side Will Make You Forget: As Peter wears the V-252, he becomes much more uncooperative and aggressive as the suit takes greater control over him. Even after he takes off the suit and very briefly has to put it on again to save the day in "Stark Expo", he briefly considers just keeping it on forever before Iron Man and Max talk him out of it.
- Geek Physique: Unlike most versions of the character, Peter didn't gain much of an increase in muscle mass or tone from his mutation. He still weighs a paltry 97 pounds even though he stands at 5'10''.
- Hero with Bad Publicity: He enters this territory in season 2 when J. Jonah Jameson shows up, complaining about the collateral damage in his wake.
- I Have Many Names: Peter Parker, Spider-Man, Spidey, Stealth Spider.
- Indy Ploy: Sometimes, most notably when he dives out a window to save Max Modell while not actually being sure whether or not his new parachute web function actually worked or not.
- I Shall Taunt You: Come on, it wouldn't be Spider-Man without this.
- It Began with a Twist of Fate: His mutation arose after being bitten by an escaped radioactive spider while attending an Oscorp demonstration. Anyone could have gotten spider powers if Harry hadn't volunteered him for the job.
- Kid Hero: Barely meets the criteria as a 15-year old.
- Leitmotif: Often accompanied by a loud, heroic orchestral piece as he engages his enemies.
- Locked Out of the Loop: He's not filled in about what Doc Ock did while their minds were swapped (i.e. quitting Horizon High, tarnishing his reputation with un-Peter-like behavior, getting J. Jonah Jameson's approval as Spider-Man, and encouraging Aunt May to eat healthier), leaving him baffled until Miles explains what happened.
- The Mentor: Becomes this, as both Peter and Spider-Man, to Miles after he gets his powers. Peter does note that he has all of three months' experience, and isn't exactly a veteran. Miles counters that he needs a mentor with his powers, which narrows his options down to... Peter.Peter: I'm...flattered?
- Motor Mouth: As both Peter and Spider-Man, he never shuts up.
- Muscles Are Meaningless: His proportionate Spider-Strength allows him to lift and punch well above what his wiry frame would suggest. According to Iron Man, Pete only weighs 97 pounds, which would be considered very underweight for a boy his age. It doesn't stop him from catching cars and helicopters.
- My God, What Have I Done?: Outside of the "My God" part, Peter literally says this upon realizing that Ben's killer was the guy he let get away earlier.
- Omnidisciplinary Scientist: Peter is not only gifted in every kind of physical science, but is also a skilled enough computer programmer to design his upgraded costume and program all sorts of functions, sensors, and scanners into it.
- Relative Button: Don't disrespect Uncle Ben, as Rocket Raccoon found out the hard way.
- Sesquipedalian Loquaciousness: He doesn't get brain freeze. He gets sphenopalatine ganglioneuralgia.
- Super Soldier: Unlike most versions of Spider-Man, Raymond Warren intentionally created the genetically modified spider that bit Peter to create an army of spider-powered super soldiers. Peter just happened to be in the right place at the right time after one of them escaped.
- Techno Babble: With the show's emphasis on science, this Peter engages in this more than other incarnations.
- Teen Genius: Peter is exceptionally gifted in math and science, as best seen when he quickly neutralized a haywire experiment involving vibranium by pouring the corresponding materials into the machine in the correct order. However, while the show goes out of its way to emphasize this, it's also downplayed by the fact that he goes to a school for geniuses, meaning that he comes across as a Normal Fish in a Tiny Pond at times.
- Younger and Hipper: While Peter canonically started his superhero career as a teenager, this version borrows from Dan Slott's Spider-Man, including Horizon Labs being reimagined as a special high school he gets accepted into opposed to a lab he works at as an adult and the updated costume being a modified version of the Spider-Armor Mk IV from All-New, All-Different Marvel rather than a normal cloth costume.
- Voiced by: Nadji Jeter
- Adaptational Badass: His venom blast from the comics goes from Fingerpoke Of Doom to electrical energy blast that can total two giant robots to instant submission.
- Adaptation Personality Change: Since the context in which he began as Spider-Man from Ultimate Marvel is no longer there (i.e. he became Spider-Man after Ultimate Peter temporarily died, and indeed becomes Spider-Man while Peter is still a teenager, Miles's show personality has changed, going from being nervous and reluctant about being a Legacy Character to being a superhero who is a Thrill Seeker and goofball who eventually mellows under Spider-Man's mentorship.
- Even beyond the aforementioned context of how he became Spider-Man, Miles is canonically an introvert, albeit a more realistic version who's functional, as opposed to the more typical Hollywood versions. It is an occasional point of contention that this is changed Depending on the Writer, with the implication that healthy extroverts are easier/more familiar to write than healthy introverts. This show completely upends this part of Miles' character, making him a full-on extroverted person, once again, regardless of the context of how he becomes Spider-Man.
- Adaptational Intelligence: While Miles is bright and canonically a student at a STEM school, in the comics he got there through a lottery. Here, he's shown to have built a security robot.
- Adorkable: His more childlike personality compared to Peter makes him quite endearing.
- Age Lift: Miles Morales is 15 at the time Spider-Man makes his debut rather than 12/13.
- Alliterative Name: Miles Morales.
- Badass Bookworm: A given since Horizon is a genius school. Miles designed a working robot.
- Cannot Keep a Secret: Blurts his secret identity to Peter, Anya, and Gwen within minutes of finishing his costume, and the only reason he didn't tell more people is because Peter convinced him to keep it a secret.
- He gets better after absentmindedly revealing where he lives on the Evening News and endangering his neighborhood and his father.
- Comically Missing the Point: Once he obtains his spider powers, the lessons Peter tries to instill into him about secrets, power, and responsibility go over his head until the Smythes send their respective Spider Slayers to capture him.
- Dark Is Not Evil: His costume is black and red and he can use red lightning, but he is still one of the heroes.
- How Do I Shot Web?: Literally until he learned that they were tech based.
- Jumped at the Call: Literally when he saves Spider-Man.
- Keet: Once he gains his super-powers, he gets really excitable and eager to use them.
- Kid-Appeal Character: He's usually the first one to crack a joke in most situations, is more excitable than Peter in more mundane ways, might even be slightly younger than him, along with being relatively new to superheroics.
- Secret Public Identity: He's never adopted his own superhero alias and Peter calls him "Miles" even when they're in the middle of fighting villains.
- Secret Keeper: In "Kraven's Big Hunt", he finds out Peter's secret identity as Spider-Man and promises not to tell anyone at school.
- Story-Breaker Power: Due to the fact that most of the villains Spider-Man faces in this series wear Powered Armor or use heavily electronic weaponry, this trope is likely why Miles sporadically does any heroing in the series; his powers, which can short out electrical devices, would make capturing a number of these tech-based villains too easy.
- Out of Focus: Tends to come off as this, likely due to the above-mentioned Trope. It gets even more frustrating when he does nothing to help Peter during the "Bring on the Bad Guys" arc.
- Voiced by: Melanie Minichino
- Adorkable: While not to the extent of Peter and Gwen, she has her moments. Notably, when Miles gets spider powers, she geeks out quite adorably at the possibility of all of them getting spider powers.
- Age Lift: Played with as while she's a teenager like her comics counterpart, comics Anya was introduced after Peter had finished college and was thus older than her. Here, they're the same age.
- Ambiguously Bi: While rather heat of the moment, she clearly says to Miles during the Spider Island arc that she could kiss him for the good job he did. At the same time, she's shown to have a very special connection to Gwen in said arc.
- Badass Bookworm: A given since Horizon is a genius school. She designed a scrambler that cuts the power to any electronic equipment.
- Brought Down to Normal: Subverted. Anya is the only person to have kept her spider powers after Spider Island.
- Cute Bruiser: She won't hesitate to get into a physical fight if there's danger around.
- Dark Is Not Evil: Her Spider-Girl has the same motif and colors as the Black Suit/Venom and is one of the heroes.
- Foil: She serves as one to Gwen Stacy. Compared to Gwen, who becomes more open hearted, and is more of herself out the costume than in it (on top of being more emotionally vulnerable), she tends to be more cynical, has some skewed priorities, yet is also shown to be more level headed when it comes to heroics, with her ego downplayed.
- Jerk with a Heart of Gold: She may be stern and ambition driven, but she won't hesitate to leap into action to help others.
- How Do I Shot Web?: Literally; out of all the protagonists, she is by far the one who has the most trouble getting used to her spider-power when she gets them, and is freaked out by it.
- Never Trust a Trailer: Promotinal materials featured her in a version of her Spider-Girl costume with inverted colors on the torso (a black spider on a white torso). Not only does she wear a more-accurately colored costume (white spider on a black torso), but she didn't even don the identity until season 2.
- Not So Above It All: In "Dead Man's Party", she joins Miles in doing the robot.
- Secret Keeper: She and Gwen know Miles's secret identity, but not Peter's.
- Skewed Priorities: She chews out Peter for messing with her device, seemingly not knowing that Midtown High would've been destroyed by her invention had he not stopped it.
- Socially Awkward Hero: To a degree. She was shown to be very against the school dance that Peter accidentally came up with in "Party Animals" and was absent for the party in "Halloween Moon" (Miles at least has the excuse of possibly training). She seems to have grown out of it in season 2, judging by her willingly attending a Ross Caliban concert and going to Peter's party.
- Tomboy and Girly Girl: The Tomboy to Gwen's Girly Girl.
- What the Hell, Hero?: In the second episode, she calls Harry out for not being appreciative of Spider-Man saving his life from a Spider Slayer.
- 100% Adoration Rating: Whereas most Spiders tend to be Heroes With Bad Publicity, Gwen, operating without a mask or secret identity, has quickly become the darling of Manhattan as Spider-Gwen.
- Academic Alpha Bitch: Known as "the Princess of Preparation" at school, she is very smart and she likes to flaunt it with little false modesty. This does reflect parts of Gwen's early appearances, but differs in both kind and degreenote . Becomes a much more lovable type after she bonds with Peter following her uncle's arrest.
- Acquired Situational Narcissism: Even after becoming a more humble person than she was at the start, Gwen can't help but relapse into being somewhat cocky when she first becomes Spider-Gwen, a hero with no secret identity, and everyone promptly adores her for it .
- Adaptation Personality Change: Like most adaptations of Gwen Stacy, her personality has no resemblance to her original comics appearance (beyond the reworked variant on her initial snobbishness as stated above) — she's especially different from the comics' Spider-Gwen who's an underdog musician — and she's more or less an original character who just so happens to be named Gwen Stacy.
- Adorkable: When joining in on super-heroics, she's less composed than usual and lets her cute, dorky side show more.
- Broken Pedestal: Her uncle, Raymond Warren, was her mentor who inspired her to get into science and she is disappointed when he is revealed to be a super-villain.
- Character Development: At the start, Gwen thinks Spider-Man is a bungler who causes more problems than he solves. Her opinion of him improves over the course of the first season, and by the time of "Spider Island", she is passionately defending him from an angry Harry, saying that Spider-Man isn't a bad guy and only ever tries to do the right thing. She also grows a closer bond with Peter Parker, due to how much he's influenced and supported her.
- Complaining About Rescues They Don't Like: She isn't a big fan of Spidey at the start, and points out what he could have done better any time he saves the day.
- Composite Character: She has the look and fashion of the mainstream Gwen Stacy, the scientific intelligence of the Amazing Spider-Man films (here taken to genius levels), and the powers of Spider-Gwen.
- A Day in the Limelight: She has a major role in the season 2 episode "The Day Without Spider-Man".
- Foil: To Anya Corazon. While Anya has some skewed priorities and is more more comfortable with heroics, Gwen is the opposite. She's very altruistic when out of costume, but when it comes to major tragedies and being in costume, she shows more emotional vulnerability.
- Helmets Are Hardly Heroic: Or rather Masks Are Hardly Heroic. While Gwen wears her Spider-Gwen costume in the first season like in the comics, she is lacking the mask. Averted in the second season.
- Hourglass Plot: Her first appearance has her criticizing Spider-Man ever chance she got. After she gets spider powers of her own, Spider-Man is the one who points out all her faults. The only difference is that she cheekily waves off his comments.
- Little Miss Snarker: Frequently snarks at people, whether she likes them or not. She even does it to the Hulk.
- Loves My Alter Ego: In a reverse on the usual trope, it's Peter that Gwen is nice to and appreciates. Conversely, she doesn't seem to care too much for Spider-Man at first.
- Nice Girl: She eventually becomes this after "Party Animals". Snarky and no nonsense that she is, she's also the most willing to help others.
- Secret Keeper: She and Anya know Miles's secret identity, but not Peter's.
- She's also the only one who knows about Anya keeping her powers from Spider-Island.
- Spared by the Adaptation: This version of Gwen Stacy is not imported from an alternate universe. Rather, she is alive in this continuity and becomes Spider-Gwen. And given the shows target demographic, an adaptation of The Night Gwen Stacy Died is unlikely.
- Tomboy and Girly Girl: The Girly Girl to Anya's Tomboy.
- Took a Level in Badass: From Gwen to Spider-Gwen.
- Took a Level in Kindness: Her Academic Alpha Bitch side only really shows up in her first episode; the following ones, particularly following "Party Animals", downplay it considerably, depicting her as still somewhat academic, pretty nice to Peter and not particularly hostile to Spider-Man after he helps save her from her uncle. That said, she still maintains a snarky side.
- What the Hell, Hero?: And boy does she bring down Harry hard during the "Spider-Island" arc. She tells Harry to knock it off with his treatment of Spider-Man, telling him Spider-Man has been trying his hardest to make things right, did his best to save his father, and tells him that his sentiment towards any spiderperson is completely baseless and misguided.
- You Don't Look Like You: Outside of being a Caucasian female with blonde hair and blue eyes, she does not visually resemble her comic book counterpart and other versions that much, mainly through having a different hairstyle and lacking her iconic headband. Her Spider-Gwen costume in Season 1 lacks the mask. She does get a wardrobe upgrade in Season 2, wearing a blue shirt instead of a pink one, and wearing her hair in a ponytail instead of a bun and finally wears a mask for her super alter ego.
- Adaptation Dye-Job: His costume is primarily navy blue in place of the blacks of his comic costume. Averted later on as his costume gradually darkens to his comic costume's shade of black coinciding with him slipping into Knight Templar territory.
- Age Lift: While Peter was an adult when Otto took over Spider-Man's body, Peter is a teenager. Otto himself is also significantly younger than his comic counterpart.
- Anti-Hero: Through Peter's memories, Otto finally understands the value of altruism. Despite now desiring to become a hero and understanding With Great Power Comes Great Responsibility, he still hasn't lost his old ruthlessness or his ego for that matter. It's demonstrated rather brutally in "Critical Update" when he outright tries to kill Sand Girl for daring to oppose him only to be stopped by Miles. He was also willing to let an innocent man dangle on the edge of a scaffolding in order to keep Miles off his back.
- Black-and-White Morality: In "Cloak and Dagger", he admits that the eponymous duo suffered Tiberius Stone's horrific experiments, and that Stone will be made to pay for these crimes, but adds that they are also guilty of criminal activities, and he will bring the two down too.
- Black Eyes of Evil: Well, more like Black Eyes of Anti-Heroism. His costume's eyes are black, in contrast to Peter, Miles, and Anya's white.
- Chronic Hero Syndrome: As a consequence of retaining Peter's memories including the death of Uncle Ben, he finds himself incapable of resisting the urge to help those in need even when it inconveniences him. Of course, he proves to be a lot more flexible with this newfound urge, considering he willingly passed by a man dangling on a scaffolding just because he knew Miles would be following behind him and intervene on his behalf.
- Composite Character: His spider-symbol closely resembles the Iron Spider suit in the Marvel Cinematic Universe.
- Did Not See That Coming: He admits to himself that he miscalculated how much Peter's residual memories would end up affecting him.
- "Freaky Friday" Flip: He's Otto Octavius in Peter Parker's body. However, he still does retain at least some of Peter's memories, which are what drive him to be a hero in the first place.
- Good Feels Good: His reaction when people thank him for saving their lives. It seems that this is a genuinely foreign feeling for him.
- Hazy Feel Turn: Otto initially took over Peter's body with the intent of using Spider-Man's powers for conquest. Peter's memories end up teaching him the value of heroism, forcing him out of his previous villainous mindset. However, this leads him to sliding into Knight Templar territory instead. He cements his turn to good in "Superior" by agreeing to swap back with Peter so Peter can face Venom.
- Heroic Sacrifice: He swaps back with Peter so that Peter can successfully deal with Venom, even though this means going back into a dying mechanical body.
- Insufferable Genius: He retains his incredibly high opinion on his intelligence and is more than willing to lord over others with it.
- Knight Templar: He does seem to earnestly want to do good. Unfortunately, "Critical Update" shows that he's more than willing to impose his views on how the world should work onto those he deems his opposition.
- Late-Arrival Spoiler: The promotional material for season 2's second half doesn't even bother to hide his origin.
- Lighter and Softer: Barring an interrupted attempt to shatter Keemia Marko after turning into glass, SpOck doesn't murder anyone in this adaptation.
- Nice Job Breaking It, Hero!: His aggressive takedown of every major supervillain in New York creates a power vacuum that allows the much more dangerous and unified Goblin Nation to emerge.
- Not Himself: Otto doesn't even try to act like Peter. Other characters quickly notice this, though they're in the dark as to the sudden shift in Peter's personality.
- Not His Sled: Unlike the comics version who completely erased himself to allow Peter's restoration and thus losing out on his Character Development, Peter ends up saving Otto and returns him to his body, hopefully with his Character Development intact.
- Terror Hero: Upon taking up the Superior Spider-Man mantle, Otto conducts himself more like a slasher villain, sticking to the shadows and brutally picking off mooks one by one.
- Ungrateful Bastard:
- Shows no gratitude whatsoever when Miles saves him from Sandgirl's trap in "Critical Update".
- He does the same after Peter saves him from Stone.
- Voices Are Mental: Averted. Though Scott Menville voices his internal monologues, he still retains Peter's speaking voice (Robbie Draymond), albeit with Otto's distinct cadence and accent.
- What Is This Thing You Call "Love"?: In his whole life, no one has liked Otto, much less loved him. The warmth and grief in Peter's memories of Uncle Ben are so foreign that they successfully overwhelm him in just a few hours.
Harold "Harry" Osborn
- Voiced by: Max Mittelman
- Adaptation Dye-Job: Harry Osborn is traditionally depicted as brown or red-haired in the comics and most versions. Here, his hair is black.
- Adaptational Attractiveness: As in the Ultimate, Ultimate Spider-Man and the film versions, Harry is more good looking here as opposed to his nebbish look in the original comics, Spider-Man: The Animated Series and The Spectacular Spider-Man.
- Adaptational Intelligence: Not that Harry was dumb to begin within the comics, but he's just as enthusiastic about science as Peter is and was admitted into Horizon High, a high school "for geniuses" while Peter didn't at the time. He also created most of the Goblin technology he uses as the Hobgoblin himself.
- Broken Pedestal: He is very heartbroken to learn that his father has been conspiring against Peter all along, realizing that Peter's claims of him getting suspended from Horizon High on purpose were true.
- The Bus Came Back: After being Put on a Bus, Harry finally returns in the "Goblin War" arc.
- Character Development: He's the most vocal in his distrust of Spider-Man when the series starts, even when reluctantly working with him, and it gets worse when the Jackal attacks Oscorp, making him think the two are working together. After the events of Spider-Island, where Gwen gets spider powers and calls him out for turning on her and Peter reveals that he's Spider-Man, he, after getting over his feelings of betrayal, puts much more faith in Spider-Man, and stops trying to justify why he could be evil.
- Cool Sword: Creates one that forms a blade out of fire.
- Composite Character: He is an amalgamation of his comic counterpart, Phil Urich and Tiberius Stone. Like both his original and Ultimate counterparts, Harry is Peter's and Gwen's friend, and Norman's son and there's even his thermal jumpsuit and mask which resembles the New Goblin. Phil's post-FaceHeel Turn is fused into his character as he knows Anya and his look evokes a more spiky black hair, wields a flame sword, and his Hobgoblin alter ego sports a sonic scream. His engineer-level intelligence, association with Modell, and Spider-Slayer usage is similar to Tiberius.
- Deuteragonist: Outside of the Spider heroes, he gets the most screentime, plot importance, and characterization in Season 1 out of Peter's circle of friends.
- This makes his Out of Focus period starting in Season 2 all the more jarring, both because he was pretty much the secondary main character throughout Season 1 (one of the biggest things Peter had that Harry didn't was being The Narrator) and especially since it happens after the point where he's reconciled with Peter and where one might assume that would lead to the two working in active consort with one another.
- Green-Eyed Monster: At least part of his Irrational Hatred for Spider-Man is his desire to have a chance to be in the spotlight as a hero the way Spider-Man is.
- Hates My Secret Identity: Of all the civilian characters, he easily dislikes Spider-Man the most despite being best friends with Peter Parker, though not as much as his dad.
- Horrible Judge of Character: When the Horizon High demonstration at Midtown is sabotaged resulting in his suspension, he thinks Spider-Man (who saved everyone) is responsible while believing the Smythes (the real culprits) wouldn't sink so low. His opinion on Spider-Man is so low that, after learning about the Jackal's plans for an army of people with Spider powers, he immediately assumes that Spider-Man is The Dragon to The Jackal after the latter gives a sarcastic compliment to the former, and this assumption refuses to disappear even when Spider-Man is clearly shown fighting him afterward. Seems to be getting somewhat better about it after "Spider Island Part 1", though, after he loudly states he thinks Gwen might be part of the Jackal's Spider army to her face, she tells him that he's being irrational, and he makes a clearer, if still begrudging, attempt to just trust Spider-Man, and even tries to repair his friendship with Peter.
- Hypocrite: During the "Rise of Doc Ock" storyline, he finds out about Peter's "alliance" with Spider-Man, and breaks off their friendship, angry that Peter had lied to him. But not long after, he pretends to want to reconcile their friendship in order to trick Peter into helping him find the Jackal's lab.
- "I Know You Are in There Somewhere" Fight: After learning that Doc Ock has brainwashed Spider-Man into joining the Sinister Six, Harry drops all his hatred and tries to reach him to break him out of the mind control. He succeeds.
- Irony: He values his friendship with Peter, but distrusts Spider-Man.
- Irrational Hatred: No matter how many people Spider-Man continues to save, Harry refuses to see him in a positive light. Until the "Spider Island" arc after Gwen calls him out, and after he finds out Peter is Spider-Man. This hatred is dialed Up to Eleven when Peter lets Oz Academy get destroyed even though it can always be rebuilt and that it helped cure everyone in the city.
- Jerk with a Heart of Gold: Harry may have an irrational hatred of Spider-Man, and can be morally skewed, but is ultimately a good person. A good example is in the last two parts of "Spider Island", where he's understandably upset about Peter not telling him that he's Spider-Man, but at least tries to make amends with him when he sees it he good he did in saving his father. "The Hobgoblin" expands on this where he's still mad at Peter for letting Osborn Academy blow up when trying to save the city, but has a change of heart when he sees a brainwashed Peter committing crimes, is initially mad at him, but then tries to get to him when he sees the truth. He's got quite a bit to do, but he's getting better.
- Mythology Gag: His Hobgoblin alter ego is similar to his Ultimate counterpart in the Ultimate Spider-Man comics.
- The Not-Love Interest: Similar to his Ultimate Spider-Man incarnation. Harry's friendship with Peter, and how it's affected by Peter's supposed flakiness, is the focus of multiple episodes and Peter's most important relationship in the show so far.
- Put on a Bus: Season 2 has Harry running Oscorp overseas. At least until the second half.
- Secret Keeper: He finds out that Peter is Spider-Man in "Spider Island Part 4", and was willing to keep it even though they weren't on the best of terms.
- Stubborn Mule: For most of the first season, Harry refused to see Spider-Man as anything other than a menace, despite witnessing several of his heroic deeds with his own eyes. Even when Screwball (whom Harry had admired) publically announced that Spider-Man was a real hero, he would not be swayed.
- Uncle Pennybags: Wants to be this for Peter, bringing him lunch and breakfast and offering to help him cover the tuition costs of attending Horizon High, but Peter turns him down on the basis that it wouldn't feel right for him to be using his connections that way.
- Ungrateful Bastard: Rather than show appreciation for being saved from the Spider Slayer in the second episode, he accuses Spider-Man of trying to kill him. Subsequent episodes have him develop a grudge against the web slinger. He reaches his lowest point in the "Spider Island" arc, when he lashes out at Peter for getting Osborn Academy destroyed, even when New York is saved.
- "Well Done, Son!" Guy: He really wants his father's appreciation, going out of his way to try and impress Norman at every turn with his increasingly over the top and dangerous inventions. But, surprise, surprise, poor Harry doesn't get any of it because his dad is Norman Osborn.
- We Used to Be Friends: In "The Rise of Doc Ock, Part 4", he ends his friendship with Peter after he gets fed up with him backing up Spider-Man, and again during the fifth part of "Spider Island". Thankfully it is rekindled in the "Hobgoblin" arc.
- Abusive Dad: Played with example, but to start with, he frames Harry for a crime his son did not commit which is considered abusive for a father to do (even though he instructed someone else to do it), not to mention saying that Harry wouldn't have anything without the Osborn name and discouraging from trying to establish himself outside of it, but some episodes like "Halloween Moon" shows that Norman cares for his son's safety.
- Adaptational Dumbass: Downplayed. While he's still a very adept manipulator, he gets blindsided by his arrogance more often than in other incarnations, such as failing to realize Otto Octavius was going to betray him as soon as he said that he owns Otto's tech.
- Adaptational Heroism: To a lesser extent; while he is still as unscrupulous and ruthless as usual, his primary motivation here is to stop the Jackal from creating an army of spider-themed Super Soldiers, an unusually altruistic goal for his character. He also antagonizes Spider-Man not out of greed or revenge as usual, but because he wrongly believes Spider-Man to be working for the Jackal. Though it's ultimately subverted on the last part: turns out he wanted Harry to become a hero for the new generation, only for Spider-Man to show up at the wrong time and steal his thunder, so he planned to get rid of Spider-Man.
- Anti-Villain: Surprisingly. He is ruthless, manipulative, deceptive and corrupt, but he genuinely wishes to prevent Jackal's army of spider-soldiers.
- Back from the Dead: Seemingly returns in Season 2 as the Goblin King. Subverted, as while his actual status remains uncertain, he isn't the Goblin King. Adrian Toomes is, and made no attempts make the heroes think it was Norman. They just had the wrong assumption.
- Big Bad: Of Season 1, most of the things that go wrong and villains that are created can be traced directly to him.
- Big Bad Ensemble: He ends up in conflict with Season 1's other major villain: Raymond Warren/The Jackal.
- Create Your Own Hero: The main reason he had Harry suspended form Horizon High and created Osborn Academy was to turn Harry as well as the Osborn Commandos into "true" heroes for the younger generation.
- Comic-Book Movies Don't Use Codenames: He never took on the Green Goblin alter ego during season 1 though he did take on two different identities (Spider-King and Hobgoblin) during season 1.
- Composite Character:
- As the "Stealth Spider"/Spider-King, he is a placeholder for both Kaine Parker and the original Spider-King.
- His Hobgoblin impersonation is a mix of Roderick Kingsley and Phil Urich.
- Corrupt Corporate Executive: It is Norman Osborn, after all.
- Devil in Plain Sight: Even at his most Faux Affable, the guy's every word and action has "evil mastermind" written all over it.
- Driven by Envy: Why he framed his own son; he was envious of Max being something greater than the Osborn name.
- Even Evil Has Loved Ones: Can be stern and a bit controlling with Harry but loves his son all the same, despite being a villain.
- Even Evil Has Standards: The Jackal approached Norman about working together on an army of spider soldiers and got turned down. Not only that but Norman is viciously driven to prevent said army's creation and opts for destroying the resources that would enable the plan rather than taking for his own use.
- Evil Counterpart:
- To Max Modell. Whereas Max prefers to work ethically and students to work together, Norman doesn't care about ethics and encourages competition between each other, even at its most extreme.
- To Uncle Ben: whereas Ben taught Peter to use talents to help others in his own way, Norman tries to mold his son into a direction he prefers.
- Evil Is One Big, Happy Family: Norman welcomes all to his faculty those who are morally bankrupt, or who feel betrayed by Modell for denying a chance at their work.
- Faux Affably Evil: He keeps a courteous and pleasant tone while talking with Modell, but it's pretty clear that behind this thin-veiled facade, he has very clear disdain for the man. Most of the other characters who personally know Norman know it's an act immediately.
- Fiction 500: Casually founds an entire academy filled with state-of-the-art technology in virtually no time at all. Then again, he is the CEO of Oscorp, a rival of various industries.
- For Science!: He's more than willing to manipulate two teenagers who are best friends into attacking each other with lethal sonic weaponry just to see the full capabilities of their creations.
- Gaslighting: Had Smythe set up Harry on purpose as an excuse to set up Osborn Academy and have enroll there, and then guilt-trips Harry and carefully derails his son's confidence to make dependent on his father's tutelage. This is a classic Norman trick common across all adaptations. Then, he does the same thing to Otto, giving incorrect information as to a cure for the Lizard, then pinning the blame on Spider-Man and leaking to news outlets that Otto made the towering Lizard that trashed the city.
- Hidden Agenda Villain: Osborn has some plan or the other, involving Warren's experiments on spiders, originally hoping to create super soldiers until he discontinued this research.
- Hoist by His Own Petard: His manipulations end up hurting him in a big way. After revealing he owns Octavius's technology, Octavius takes control of the Oz Commandos whilst revealing his intent to take over Norman's company.
- Hypocrite: He accuses Modell of being a Glory Hound, but one of his main reasons for setting up Osborn Academy was to prove he could make a better school than Horizon High. And the "Hobgoblin" two-parter reveals that he hates Spider-Man far more for becoming a hero before Harry could, essentially because it robbed the Osborn legacy of such a powerful figure.
- I Have No Son!: "Then you failed me, and deserve what you've wrought!"
- Jerkass: He's a terrible person and is very upfront about it.
- Jerk with a Heart of Jerk: Norman thinks he is a Jerk with a Heart of Gold with his claims on being stern and tough yet ultimately wanting Harry to do good, but deep down, he's vile and cruel.
- Kick the Dog: He deliberately sets Herman and Clayton against each other after he sees that they're a team. Then he sends Spencer Smythe's son against his father.
- Leitmotif: Often accompanied by a quiet, eerie theme whenever he shows up.
- Manipulative Bastard: Can get most people to do what he wants simply by talking to them and preying on their fears, insecurities, and desires, letting him get away with any one of his "experiments" since he didn't have to get his own hands dirty.
- Mythology Gag: His Hobgoblin impersonation is similar to his mainstream counterpart's brief one in the Superior Spider-Man comics.
- Never Found the Body: His body wasnt found, it leads to Peter and Harry believing he is not only alive but the Goblin King. Hes not the Goblin King at least.
- Not His Sled: He doesn't use the Green Goblin moniker in this continuity. Instead, it's Toomes who adopts a variation of the moniker and does attempt to impersonate Norman for a brief moment to throw Harry and Spider-Man off.
- The Resenter: Big time. He wanted his son Harry to become the hero of the new generation. But after Spider-Man showed up he came to the conclusion that Spider-Man was stealing his son's thunder and made several plots to get rid of Spider-Man and get the Osborn name into the spotlight.
- Saying Too Much: While attacking as the Hobgoblin, he told Spider-Man he would learn the web-slinger's secret identity before killing making Spider-Man realize that it wasn't Harry in the Hobgoblin suit, since Harry already knew Peter's true identity.
- Stage Dad: Wants to mold Harry into his idea of the hero that New York City needs, and got Harry kicked out of Horizon and set up Osborn Academy so he could keep a closer eye on and mentor himself instead of letting Max influence him.
- Start My Own: After Harry's suspended from Horizon High, it's mentions that his dad wants to start his own, better school. He makes due on that promise in "Osborn Academy", and it's out of spite for how Modell and the other students at Horizon treated Harry. And, given the plot to frame Harry was his idea, Norman was planning it from the start.
- Ungrateful Bastard: Max officially clears Harry of all charges, and how does Norman repay Horizon High? Burns all bridges with them by opening Osborn Academy and convinces Harry not to go back to Horizon, all because he can't forgive them for not believing in Harry. The fact that it was Norman who framed Harry and started the situation is all the more hypocritical and cynical.
- Villainous Breakdown:
- In "The Rise of Doc Ock, Part 4", when he finds out that the Oz Commandos were no longer under his control results in him (for the first time in the series) stumbling, losing his coolheadedness (seen when he tries to kill the four experimental Spiders), and being legitimately afraid for his life.
- In "The Hobgoblin", he drops his Faux Affably Evil act after being discovered as the Hobgoblin promptly trying to kill Spider-Man and then viciously disowns his son for refusing to follow his orders.
- Villain Has a Point:
- Despite the nefarious purpose behind his school, it's hard to argue against him when he mentions that Harry's classmates didn't stand by him when he was accused. Max in particular, while being a Reasonable Authority Figure about the whole thing and being smart enough to recognize Smythe framing him, still chose to suspend Harry before he absolutely had to. Then again, the whole plot to frame Harry was Norman's idea, anyway. Nonetheless, the fact that those who knew Harry for the longest time didn't believe in his innocence in such a situation justifies that sentiment.
- He also didn't want Harry to debut his flying glider at the Stark Expo. Though Norman probably had his own agenda for the glider, he was still right to tell Harry not to present it there, considering a supervillain hijacked the expo and destroyed almost everybody's experiments there. Harry later understood afterwards.
- Although Max is the one who acknowledges it, no matter how morally bankrupt people see Norman as, he has the right to run his school as he sees fit, so Max has no say in the matter.
- Waistcoat of Style: Norman sports one constantly, rather than the usual double-breasted suits he wears.
- Voiced by: Max Mittelman
- Adaptational Heroism: Played with. While most incarnations of Hobgoblin are villains, Harry Osborn is willing to be a hero as opposed to his Ultimate counterpart who was a Tragic Villain.
- Adapted Out: Roderick Kingsley and Phil Urich are nowhere to be seen.
- Composite Character: This depiction is an amalgamation of various iterations. Season 1 shows a more technological design of Kingsley's attire with Phil's flame sword and sonic scream. Season 2 has a more streamlined design which resembles the New Goblin.
- Voiced by: Scott Menville
- Abusive Parents: During his childhood, his father Torbert was shown to be both physically and verbally abusive to Otto, such as pushing and belittling him about science.
- Acting Your Intellectual Age: Despite being nineteen, which is very young for an adult, he acts and talks to students as if he's even older.
- Actually, I Am Him: When Peter first meets him, Peter mistakes Otto for a fellow student and mentions the bad things heard about Otto. It's only upon seeing a picture of Otto receiving an award from the Mayor that Peter realizes of talking to Otto.
- Adaptational Heroism: While his comic counterpart always was a Noble Demon and did try to act as a hero during a specific arc, he started out as a straight villain and was one for most of his career. This incarnation, while definitely a Jerkass with ego problems, starts out as a hero before his ego gets the better of him in season 1. He also doesn't snap and go psychopathic after his freak accident like most versions of his character did, instead continuing his career as a teacher until the tensions with Modell lead him to quit. In season 2, he also makes a genuine attempt to reform after giving Peter's body back, and dies a hero in season 2's finale.
- Adorkable: During his brief time as a hero, he makes some attempts at quipping along with Miles and Peter. The results are... mixed, but amusing.
- Alliterative Name: Otto Octavius.
- Appropriated Appellation: Implied. Briefly in "The Rise of Doc Ock, Part 3", Peter refers to the Oz Commandos as "sinister". In the very next part, after revealing that he used mind control on the other members, he refers to the group as the "Sinister Five".
- The Bad Guy Wins: At the end of "The Living Brain", he successfully takes over Spider-Man's body after transferring his consciousness from the titular robot. Guess where Spidey's consciousness is now?
- Beware the Quiet Ones: While Otto's definitely an Insufferable Genius, he does tend to dip into this on some occasions. The most recent case being the "quiet one" of the "Oz Commandos", being subservient to Osborn until the Oz Commandos are ordered to finish off the Jackal...which is where Doc Ock reveals his Manipulative Bastard qualities through Evil Gloating.
- Big Bad Ensemble: His scheming and machinations in the "Rise of Doc Ock" multi-parter puts him on the same level as Norman Osborn and the Jackal in season 1. With Norman seemingly killed and the Jackal out of the picture, Octavius is the only operating major villain of season 2's first half.
- Big Ol' Eyebrows: Has a rather big set of those.
- Body Horror: His robotic arms are merged with his nervous system now in "The Rise of Doc Ock Part 1".
- Character Development: Spending time as the Superior Spider-Man significantly softens him, putting him toward a genuine effort to become a hero (albeit much darker than Peter), and his interactions with Anna Maria and the swap being undone result in him shedding a lot of his worst tendencies as both Doctor Octopus and the Superior Spider-Man, becoming an ally to Peter as a result.
- The Chessmaster: How did he manage to get control of Peter's body? By sending wave after wave of villains after him to tire him out.
- Composite Character: His mind within the Neuro-Cortex, and followed by a robot body dubbed "The Living Brain", bearing the name and updated appearance of a separate, obscure antagonist from the comics. This also makes him one to his backup consciousness in the Living Brain.
- Cool Shades: In his pre-villain days, he occasionally wore black sunglasses through experiments and crime-fighting.
- Death by Adaptation: He sacrifices himself to save New York during the "Goblin War" arc.
- Died in Your Arms Tonight: He dies in Peter's in "Goblin War, Part 4".
- Evil Counterpart: Another one to Peter Parker/Spider-Man; they're remarkably intelligent for their age and driven to do good by a figure who gave them a pep-talk on using their abilities responsibly, but unlike Peter, Otto's ego cripples his judgment and eventually results in him being a villain. In a dark mirror to Max seeing Peter's potential and letting him into Horizon, Norman wants Octavius to be part of Oscorp, something Norman succeeds in. He also leads a team of people with great scientific minds, but instead of a mutual desire to work together, has implanted mind control devices on them to render them subservient to him.
- Evil Genius: Is miffed with Norman after discovering the Octobot project. When he confronts Norman about this, Norman nonchalantly dismisses his complaints, since Oscorp owns Otto's research contractually. To get back at Norman, Otto utilizes mind-control devices he developed alongside the Jackal in order to completely brainwash the Oz Commandos and severely wound Norman, effortlessly taking over Oscorp as well as the Jackal's own research. Damn.
- Evil Is Hammy: He was mostly a stoic grump before, but he gets more bombastic as Doctor Octopus.
- Fatal Flaw: Pride. While Otto undeniably is brillant, he is so convinced of his own genius that he dismisses everyone as less intelligent than him and doesn't even considers they might have a point. Most notably, he worked on an extremely volatile power source in a school's basement in blatant disregard for the students' security, based on the argument that surely, someone with a superior intelligence like him would know how to make it safe. Amusingly, this also is what prevents him from figuring out Spider-Man's identity; despite having recognize Peter's tech on one of Spider-Man's tracers, his assumption is that Peter is providing tech for Spider-Man rather than being Spider-Man himself, because he cannot believe Spider-Man would be smart enough to build his own tech like himself is.
- For Science!: His primary motivation. He considers any risk to be worth the pursuit of scientific advancement, and looks down on those who think otherwise.
- Freudian Excuse: He was bullied as a child for his short stature, and his father physically and emotionally abused him because he resented his intelligence and love of science. That instilled in him the constant need to prove his superiority over others that would ultimately lead him down the path of villainy.
- Go Out with a Smile: As his molecules are ripped apart by his Heroic Sacrifice, Otto can't help but smile tearfully because he finally found a best friend with Peter.
- Grand Theft Me: At the end of "The Living Brain", he transfers his consciousness into Spider-Man. With neither Miles nor Spider-Girl none the wiser.
- HeelFace Turn: "Superior" has him sacrificing himself to give Peter's body back after realizing what it truly means to be a hero. When he wakes up from his coma in "The Cellar", he seems to be on his way towards being an ally.
- Heroic Sacrifice: In "Goblin War, Part 4", he uses the Neuro Cortex to disable Adrian Toomes's mech, but at the cost of his own life.
- Hijacked by Ganon: He turns out to be the mastermind who put a bounty on Spider-Man's head in season 2, hoping to tire Peter out until he could finally enact his plan to steal Spider-Man's body.
- I Have Many Names: Otto Octavius, Doctor Octopus, Doc Ock, The Octopus, Superior Spider-Man.
- I Just Want to Be Loved: It's buried so deep even Otto's not aware of it, but all he really wants is to be accepted. Unfortunately, thanks to his father's abuse and the resulting superiority complex, he believes it doesn't come unconditionally, so he must prove he's worthy of people's acknowledgement. At the time of the series, he's long forgotten why he needs to be smarter than everybody else.
- Inferiority Superiority Complex: For all his arrogance, Otto really is looking for people recognizing his genius, something he had trouble finding due to his young age, and as such tends to follow whoever acknowledges him as brilliant. Max being the first to recognize him despite his age was what led him to join Horizon High. This is also exploited by Norman to create tension between him and Max, and get him to join Oscorp.
- Insufferable Genius: Otto is an incredibly smart and talented scientist, especially considering his age; unfortunately, he also is very arrogant, dismissive of others and constantly convinced he knows better than anyone else around him due to his intelligence.
- Intelligence = Isolation: "The Rise of Doc Ock" reveals he always was considered a freak by his peers due to his high intelligence, presumably leading to his asocial behaviour.
- Jerk with a Heart of Gold: Arrogant, dismissive of others, easily annoyed and generally an unpleasant teacher, but does occasionally compliment his students, and his bouts of heroism were genuine, if somewhat reluctant. He's later released from prison after his Sinister Six stunt was foiled, and in the months spent he gained three degrees, and seems to have Took a Level in Kindness, however the Kindness part turns out be be an act and he became a Jerk with a Heart of Jerk to use his kind facade as a way for his master plan. As of "The Cellar", however, he seems to have fully become this trope. Time will tell if it sticks.
- Love Redeems: It's ultimately his feelings for Anna Maria and desire to validate her belief that he's a hero that push him towards cementing his HeelFace Turn.
- Manipulative Bastard: After spending much of his tenure in the Oz Commandos as The Quiet One, Octavius fully embraces his supervillain status by expositing his plan to overthrow Norman as head of Oscorp, since the company owns all his research. To that end, he utilized the Jackal's mind-control research to brainwash the rest of the Commandos into his minions and formed a Villain Team-Up with the Jackal, off-screen and out of prying eyes. The plan goes off without a hitch...except, of course, for the fact that Norman apparently survives the ordeal. Still, the minimal time and effort Otto put into his plan is astounding.
- Mission Control: As of "The Road to Goblin War", Otto finds a new way to be a hero as Spider-Man's partner behind the Spider's Nest computers, headset and all, and controlling the Spider-Bots.
- Mistreatment-Induced Betrayal: He turns against Osborn after finding out Norman stole his technology by hiring him.
- Moral Myopia: After the Sinister Five are freed from his mind control and attack him, he is outraged that they would "betray" him, believing that he was helping the group to "achieve greatness".
- Never My Fault: In "The Hobgoblin, Part One", he claims that Max Modell had "practically driven" him to side with Norman Osborn.
- Never Trust a Trailer: This version of Doctor Octopus is 19 years old, only slightly older than Peter. However, a poster to promote the show◊ features the traditional middle-aged version with a bowl cut.
- No Body Left Behind: In "Goblin War, Part 4", his body fades away after he dies, leaving only his tentacles.
- Not So Above It All: Otto spends most of his earlier episodes as The Stoic, and looks down to the Spider-Men for their goofy antics. However, he himself displays a liking for catchy names (he came up himself with his two aliases and the Sinister Five name), makes attempts at witty banter during his brief career as a hero, and can at times be quite hammy.
- Older Than They Look: He is nineteen, but looks young enough that Peter initially mistakes him for a fellow student.
- Omnidisciplinary Scientist: Much like the comics, Octavius created a nuclear reactor with mechanical arms, requiring engineering and nuclar physics degrees. Somewhat justified in his return when he gained three more doctorates (at least one in neuroscience) during his time in prison, which helps the plot along significantly.
- Redemption Equals Death: In the "Goblin War" arc, he uses the Neuro Cortex to defeat Toomes to make up for his sins, but dies in Peter's arms in doing so.
- Reformed, but Rejected: Unsurprisingly, no one except Anna Maria and Peter is willing to accept his genuine turn to the side of good, especially since he faked being reformed once before, not to mention the many moral lines he crossed as a supervillain. It takes him sacrificing himself for everyone to acknowledge his turn to good.
- Secret Keeper: In the "Superior Spider-Man" arc, he learns about Peter's identity while possessing Spider-Man's body.
- Shadow Archetype: He's basically what Peter would've turned into without the kindness and support of Aunt May and Uncle Ben. Like Peter, he's a promising man of science who was bullied for his intelligence. Without anyone to support him and put him on the right path, however, Otto became obsessed with proving his superiority over those who belittled him, which evolved into a full-blown Inferiority Superiority Complex that would lead him to eventually becoming a supervillain. Upon finding out about his past, Peter notes how they're Not So Different.
- Shorter Means Smarter: Is much shorter than Peter, and quite brillant.
- Then Let Me Be Evil: Seems to be the impetus of his eventual transformation into Dr. Octopus; he was bullied by his peers for his intelligence, was unable to keep the job he has at Horizon due to Max assuming the worst of him, and then finding out that he doesn't own any of his tech anymore. These, coupled with calling his team "the Sinister Five", suggests he's decided to drop any pretense of being a hero entirely.
- The Stoic: He's not much on emotions.
- The Usurper: Is primarly this to Osborn as leader of the "Oz Commandos" (aka the Sinister Five), having severely injured him in the finale of "Rise of Doc Ock", and surprisingly this to the Jackal as well, at least in terms of his mind-control research.
- Villain Team-Up: Teams up with the Jackal to help along with his mutiny of Oscorp, although the latter seems to consider the deal more of an Enemy Mine.
- Voice for the Voiceless: He speaks for the Sinister Five who never speak since they are being mind-controlled by him.
- Voice with an Internet Connection: He becomes this for Peter, and later the other Spiders, after he reverses their bodyswap and comes out of his coma.
- Younger and Hipper: This version is only nineteen instead of a middle-aged man as in the comics and other versions.
Jackal (Raymond Warren)
- Voiced by John DiMaggio
- Actually a Doombot: The Jackal that attacked Norman and the one in prison were both clones of him, with the latter revealing this is a favored tactic for keeping the real Jackal out of the spotlight.
- Adapted Out: Miles Warren is nowhere to be seen and his Jackal persona is used by Raymond.
- Adaptation Origin Connection: He is responsible for the creation of the spiders who gave both Peter and the main cast their powers. He is also responsible for the Rhino in this version.
- Axe-Crazy: While his human self, despite still being evil, is a bit more collected, his Jackal persona displays psychopathic tendencies, including trying to kill random bystanders or threatening to dissect Spider-Man so he could figure out how his nemesis's powers work.
- Big Bad Ensemble: He ends up in conflict with season 1's other major villain Norman Osborn.
- Composite Character: In the comics, Raymond Warren is the Jackal's brother, but seeing as the Jackal's name was Miles Warren and this series has another Miles as a protagonist... yeah. This version also has a character trait from Arthur Stacy through being portrayed as Gwen's uncle.
- Even Evil Has Loved Ones: As evil as he is, he does seem to genuinely care about his niece Gwen, as he actually goes through the trouble of saving her in the middle of a crime.
- Evil Uncle: He's Gwen Stacy's uncle.
- Evil vs. Evil: Both he and Norman are ruthless and willing to do morally questionable things to reach their goals, but they end up in conflict due to Norman opposing his plan to create a spider soldier army.
- Expendable Clone: Uses these to keep himself incognito, and even the clones themselves go along with this, to the point of one in prison killing himself upon learning that another one self-destructed.
- Faux Affably Evil: Likes to have seemingly genial conversations with his opponents and co-workers, but it's clear that much of it isn't genuine at all.
- Karma Houdini: By the end of Season 1, he's still at large, even if his master plan was thwarted.
- Knight of Cerebus: Episodes involving him tend to be game changers, due to his personal connection to Gwen Stacy, and his overall influence having series-wide consequences. He's also regularly portrayed as being among the most evil of Spider-Man's enemies in the series, being far more willing to intentionally try and commit murder on innocent bystanders for seemingly no reason. He's also the villain that came the closest to succeeding at their goals throughout Season 1, with the events of Spider-Island.
- Mad Scientist: Just like in the comic, he is implied to have created the serum giving him the ability to shapeshift into a jackal-man. He also is responsible for both mutating the Rhino and creating the spiders who give the main cast their powers in this version.
- Our Werebeasts Are Different: He has the ability to switch between his human form and furry, green-haired Beast Man with jackal ears.
- Out of Focus: He's largely absent from Season 2, and left mostly unacknowledged. It takes "Bring on the Bad Guys" and "Brain Drain" for any references to his influence to be referenced but with no physical reappearance.
- Red Herring: Played with. He's introduced with almost no formal introduction in his first appearance, much like Scorpion, seemingly indicating that he's going to be a Bit Part Bad Guy, but at the end of his episode, is shown to secretly be Gwen's uncle, highlighting that he's really going to be a very important character, and in fact one of the most major antagonists of the series.
- Related in the Adaptation: In this continuity, he is Gwen Stacy's uncle.
- Send in the Clones: Just like in the comics, he has a big liking for clones. In this case, he repeatedly uses clones of himself as decoys, and intends to create an army of them with Spider-Man's abilities.
- Suicide Attack: His clones have a self-destruct feature. A Jackal and Raymond Warren clone both use it to try to kill Norman and Spider Man respectively.
- Voiced by: Ben Diskin
- Alliterative Name: Spencer Smythe.
- Bullying the Dragon: Mouths off to the Vulture only to get sent flying into a wall by his sonic weapon.
- Even Evil Has Loved Ones: "Ultimate Spider-Man" implied that he cares for his son.
- Hate Sink: Frames Harry for nearly killing everyone at Max's speech, inadvertently messes up his relationship with Peter, and considering what happens to his kid in the comics... It turns out this was exploited by Norman. Norman needed him to sabotage the project so that the former would have an excuse to place Harry into Osborn Academy.
- Kick the Dog: In the first episode he sabotages an experimental machine during Horizon High's demonstration, and frames his former student Harry, putting numerous people in danger and resulting in the latter being suspended indefinitely solely to spite Max Modell.
- Playing Gertrude: Ben Diskin, who's in his mid-30s, voices Spencer, a middle-aged man. Additionally, Spencer is the father of Alistair—and Ben Diskin is 9 years younger than Alistair's voice actor, Jason Spisak.
- Revenge: Seeks retribution against Norman for not keeping his deal with him.
- Small Name, Big Ego: In his first appearance, he considered himself superior to Max Modell, in spite of the fact that he was a high school science teacher at the time.
Vulture / Goblin King (Adrian Toomes)
- Voiced by: Alastair Duncan
- Adaptational Villainy: While he has more redeeming qualities in the comics, this version is far more vile, willing to use his minions as tools and having no problem with the idea of killing many civilians just to get to Spider-Man, making him the second most evil version of Adrian Toomes right next to his Noir counterpart.
- Age Lift: Instead of an elderly man, Toomes is presented as being more of a middle aged man with his hair still intact and with full color.
- Arc Villain: Of the "Goblin War" arc as the Goblin King.
- Brainwashed and Crazy: Becomes a member of the Sinister Five via mind control.
- Characterization Marches On: He's much more impulsive, loud, and egotistical in the pilot episode, but later appearances have shown him to be one of the few of Norman's henchmen to be relatively sane, mild-mannered, and even refuses to steal from Horizon because it'd jeopardize having just got out of prison. Given that Alistair wears a different Vulture suit and demonstrates a lot of the same traits as Toomes's first appearance, it was likely to help keep the two distinct when they both wore the Vulture technology.
- Composite Character: This version's Vulture technology is like original counterpart, but is as vile and evil as his Noir counterpart.
- As the Goblin King, he takes cues from Bart Hamilton and Phil Urich.
- The Dragon: He became Norman's top enforcer after he is bailed.
- Fatal Flaw: Pride. His inflated sense of pride is why he's the Vulture and attacks Max Modell. This trait also allows the Wake Riders to convince him to return to a life of crime in "Rise Above It All" and ultimately drive him in his crusade as the Goblin King.
- From Nobody to Nightmare: He goes from a glorified minion of Norman and Doctor Octopus to the leader of a crime movement that has practically every criminal in New York under his command as the Goblin King.
- Hypocrite: His motivation in the "Goblin War" arc is that he wishes to be recognized for his technology, which has been stolen by Oscorp. He accomplishes this by assimilating Electro's and Silvermane's inventions into the Goblin Nation, and stealing the Hobgoblin's schematics.
- Kick the Son of a Bitch: Since Spencer pretty much falls into the Hate Sink category, Vulture attacking Spencer can come off as this.
- Make Me Wanna Shout: His main Weapon of Choice in contrast to his other versions.
- Moral Myopia: During a Crossover with Avengers, Assemble!, he insisted that he wasn't a criminal, despite breaking out of jail with Ulysses Klaue's help. When Spider-Man arrived on the scene, he tried to "prove" himself by attempting to capture Klaue, wrecking Horizon's gym as he did. When Spidey pointed out all the damage he was causing, Vulture replied "And your point is?".
- Never My Fault: Max Modell fired him for stealing equipment that belonged to the school. He got pissed and decides to kill Max, blaming Max for it.
- Never Trust a Trailer: This version looks like he came out of Science Ninja Team Gatchaman. However, a poster to promote the show◊ features a design more akin to his counterpart in Spider-Man: Homecoming.
- Powered Armor: His Vulture technology grants him flight and a sonic scream. He later sports a bulkier, green Goblin armor as the Goblin King.
- Pragmatic Villainy: He's bailed out of jail in "Symbiotic Relationship", and when Norman tells him to go steal the V-252 from Horizon, he points out that he just got out of prison and doesn't want to risk getting caught again so soon.
- The Reveal: He's the Goblin King, not Norman as everyone initially assumes.
- Starter Villain: The first proper supervillain that Spider-Man fights in the series.
Scorpion (Mac Gargan)
- Voiced by Jason Spisak
- Attack of the 50-Foot Whatever: In "The Day Without Spider-Man", he grows to a larger size after getting blasted by the Bloodstone.
- Beware My Stinger Tail: As per usual with the Scorpion, his main method to fight is a gigantic tail with a stinger. He can also use it to shoot beams in this incarnation.
- Bit Part Bad Guy: In the second episode, he shows up robbing a museum without much context as to who he is or how he got his suit, just to give Spidey someone to fight in the Batman Cold Open.
- Demoted to Extra: Compared to all the other mainstay Spider-Man antagonists who show up in the series, a majority of whom have gotten an origin story. Scorpion gets no backstory or even any context as to who he is.
- Averted in Season 2, where he has a much bigger role in "The Day Without Spider-Man", where he teams up with the Tinkerer.
- Frickin' Laser Beams: This Scorpion's stinger shoots lasers rather than the venom he's infamous for in the most media.
- Make My Monster Grow: In "The Day Without Spider-Man", exposure to a mystical gem causes to grow to giant size.
- Voiced by: Grey DeLisle
- Adaptation Relationship Overhaul: She isn't romantically interested in Spider-Man as she is in other incarnations.
- Classy Cat-Burglar: A staple of the character, although this version doesn't mind stealing stuff in broad daylight.
- Didn't Think This Through: Steals a car from a local dealership, apparently not knowing car dealers intentionally keep exhibit cars at low gas.
- Expy: She can be seen as one to Catwoman, due to her cat burglar shtick and being rather playful with the hero. She's even voiced by her voice actress from the Batman: Arkham Series .
- The Gadfly: Spends her entire debut episode messing with Spidey and treating his attempts to apprehend her as if it's some big game. She also doesn't take it badly when he hands her over to the cops.
- Karma Houdini: Zig-zagged. Unlike most versions of the character, Spider-Man actually does bother getting her arrested, but it's implied she'll break out before she even makes it to jail.
- Logical Weakness: If she were to look at a mirror while her bad luck powers are active, she herself gets inflicted with misfortune.
- Winds of Destiny, Change: She can affect luck. To be more specific, inflict bad luck on anyone she looks at if she wishes to.
- Worthy Opponent: Seems eager to tangle with Spider-Man again after he manages to defeat her.
Sandgirl (Keemia Marko)
- Voiced by: Sofia Carson
Flint Marko's estranged daughter who was taken by Hammerhead.
- Adaptation Name Change: In the comics, Keemia had the last name Alvarado, but this version has her father's last name.
- Adaptational Badass: She inherits her father's abilities in this version, while her comics counterpart was a regular human girl. This aspect of her character may have been taken instead from Spider-Man: Reign.
- Adaptational Villainy: In the comics, Keemia was a Cheerful Child and supportive of her father and didn't understand that he was a villain. This version of her, however, is hostile and resentful towards him and joins Hammerhead. Although she inherits her father's powers, she outright kills him.
- Age Lift: In the comics, she was about seven or eight when she met an adult Spider-Man. Here, she's a teenager at the same time he is.
- Asshole Victim: Downplayed. She gets turned into a glass statue by Superior Spider-Man in season 2. Normally, viewers would feel bad for her given her rough life. Then again, since she murdered her father in season 1, it's hard to muster sympathy for her.
- Blinding Bangs: Her hair covers her right eye (or rather, what used to be her eye).
- The Bus Came Back: She returns in season 2, having apparently become a crime boss.
- Calling the Old Man Out: She berates her father for his criminal activities during their fight. She also outright tells him how stupid he was for bringing her anywhere near someone like Hammerhead, and then leaving her alone.
- Composite Character: She has the antagonistic and criminal traits that are usually associated with her father from the comics and other versions.
- The Dark Side Will Make You Forget: When she appears in Flint's flashback, she seems to be at most aloof towards him. When he's revealed to be working for Hammerhead, she doesn't sound completely sane anymore and is borderline berserk.
- Despair Event Horizon: By the time Spider-Man and Flint track her down, she seems to have completely given up on her dreams of studying hard and carving out a brighter future for herself than her less-educated criminal father. Instead, she accepts she has no options other to become a criminal too, and throws herself into the role with a manic, berserk fervor.
- The Dragon: Serves as this for Hammerhead.
- Even Evil Has Loved Ones: Sort of. Although she's Flint's daughter, she tells him that she hates him before crushing him into dust.
- That said, she only got mutated when she followed her father into the factory because she was frightened, and clearly thought she would be safe with him. She was tragically wrong.
- Evil Costume Switch: In Season 2, she is now shown wearing a red beanie and a red shirt.
- Evil Counterpart: To Spider-Man. She's encouraged to use her powers for personal gain by someone she considers a surrogate father, rather than using them responsibly.
- Evil Genius: During her Motive Rant, she mentions being very good at science (like seemingly everyone else in the series thus far), having studied hard in order to build a brighter future for herself than the one her father has. She also figures out her powers and how to use them much sooner than her father did.
- Eye Scream: Her right eye is made of sand as a result of her mutation, which explains her hair covering the right side of her face.
- Unlike Flint, who is able to pass for human completely, her eye seems to be in a permanent state of sand. While she can pass for a regular girl with her hair hanging over it, all it takes is a breeze to blow the hair out of the way...
- Hate Sink: While Keemia does have a Freudian Excuse for her nasty temperament, it doesn't justify her decisions, and she ends up being more vile than the original Sandman ever was. Not only does she seemingly murder her father, she also becomes a mob enforcer. And tries to kill Spider-Man as well, just for trying to help! It's pretty much satisfying to see Superior Spider-Man turn her into a glass sculpture.
- She admonishes her father for his criminal lifestyle and hates him for the accident that gave her superpowers, but ended up joining the exact same gang that tried to murder him and indirectly gave her powers that she freely uses. By the time of her return in season two, she's become a worse criminal than her father ever was (and is clearly enjoying every minute of it).
- In "Critical Update", she claims Spider-Man is "just a kid", even though she's a teenager herself.
- Jerkass: A major one. When her father and Spider-Man show up to rescue her, she rudely tells the latter the fight doesn't concern him.
- Karma Houdini: She escapes from Spider-Man after he has defeated Hammerhead and his goons and tries to capture her. She returns in season 2, still on the run from the police.
- Karma Houdini Warranty: When she finally does make another appearance, Octavius as the Superior Spider-Man sets off a trap to turn her into glass, and would have shattered her to pieces if it weren't for Miles.
- Kick the Dog: She downright kills her father in her debut episode, even though he outright refuses to fight back. This leads to Spider-Man unleashing the V-252 suit and defeating her.
- Locked Out of the Loop: She seems honestly unaware that the "accident" that gave her and her father her powers was in fact a deliberate attempt by Hammerhead to kill her father, blaming her father for it instead of her savior.
- Never My Fault: She blames her father for getting her involved in the "accident" that gave both of them superpowers, even though it was her decision to sneak out of the car and enter the building he was in.
- Crosses with Villain Has a Point, though. What kind of father would bring his teenage daughter to a place where he was planning to meet a crime boss... and then leave her all alone in the car?
- Nice Hat: Wears a green beanie. She changes it to a red one in Season 2.
- Self-Made Orphan: Seemingly kills her father in a fit of rage.
- Tomboy with a Girly Streak: Dresses in punk clothes, but is a girl.
- Tragic Monster: The reason she got mutated as well was because she was afraid to be left alone in an unfamiliar place, and so followed her father. Her Motive Rant as she attacks him during the climax reveals that she'd worked hard in order to avoid going down the same path of the criminal her father did, but now that she was a mutated freak that was All for Nothing.
- Ungrateful Bitch: She hates her father for working for a criminal in spite of everything he did was to support her. And by the time Flint and Spider-Man show up to rescue her, she attacks them relentlessly.
- What Happened to the Mouse?: In spite of being introduced in a fairly plot important episode, she's completely unaccounted for by the end of the first season, and doesn't show up in any of Hammerhead's other appearances. She finally shows up again in season 2.
- The Worf Effect: She manages to defeat her father and nearly kill Spider-Man, up until the V-252 merges with Spidey, resulting in her being on the ropes against him.
- Voiced by: Jim Cummings
A crime boss who Flint Marko used to work for.
- Big Bad Wannabe: Considering Norman and Jackal are more competent threats to Spider-Man than him.
- Brooklyn Rage: As expected at this point for the character, Hammerhead sports a Brookyln accent.
- Bullying a Dragon: Antagonizes Macklin/Blizzard and gets frozen to the wall for it.
- Butt-Monkey: Out of the recurring villains, he's the most likely to be humiliated or left in a precarious position, often thanks to his own actions (the area he's hiding out with Keemia ends up destroyed, he gets frozen by Blizzard, and doesn't disclose the importance of an encrypted thumbdrive to Absorbing Man to keep things simple, sabotaging his operations).
- The Corrupter: Turned Keemia against her father.
- The Don: He's a crime boss.
- Even Evil Has Loved Ones: While he's clearly manipulating Keemia, he has genuine love for his 8 year old son Tully.
- Quality Vs Quantity: Expresses the belief that while supervillains are more expensive than regular hoods, they also make more to make up for it.
- Pet the Dog: Downplayed. He takes in Keemia and even helps restore her after she was exposed to the same mixture of sand and toxic waste that he tried to kill her father with. That said, he also gleefully manipulated her vulnerable emotional state in order to gain her loyalty.
- You Have Failed Me: Orders the death of Marko after a ledger ends up in the hands of the cops.
- Voiced by: Ben Diskin (Season 1; vocal effects), Ben Pronsky (Season 2-3)
A substance found in outer space, with properties that enhance Spider-Man's powers and webbing.
- Adapted Out: One of the abilities the Venom Suit gave to its hosts was Peter Parker's memories. As such when Flash Thompson wore the suit, he should have known that Peter is Spider-Man, but this isn't shown to be the case here. However, once Eddie's the host, the symbiote retains the knowledge of Peter as Spider-Man.
- Adaptational Villainy: Most iterations of Venom only become villainous as a result of absorbing the negative thoughts and emotions of their previous hosts and typically only fixate on Peter and Eddie at most. This version is revealed in "Web of Venom" to actually be a scout for a full-blown Alien Invasion.
- Aliens Are Bastards: It's revealed in "Web of Venom" that it intentionally crash landed on Earth to signal an Alien Invasion.
- Arc Villain: The V-252 itself in season 1 takes center stage in "Sandman", "Symbiotic Relationship", and "Stark Expo", before being separated by Peter. Five episodes later, it returns in "Venom". In season 2, it bonds with Eddie Brock for "Dead Man's Party" and "Venom Returns".
- Being Tortured Makes You Evil: Being trapped in a jar for over 3 years and being experimented on has likely made the symbiote not very friendly towards humans. Venom kidnaps Max Modell to get revenge for the mistreatment.
- The Corrupter: Peter gradually loses his moral compass and starts getting more aggressive and egotistical, the more the Symbiote is on him.
- Evil Sounds Deep: As per tradition with the character, his voice (as Eddie-Venom) is particularly deep, with an alien filter on top.
- Interim Villain: In season 2, Venom takes over as the main villain when Doc Ock is put in a coma. Once Venom is defeated, Doc Ock returns to Big Bad status.
- Knight of Cerebus: Same as it was in Ultimate Spider-Man, his presence often darkens the show's fairly lighthearted tone, especially after bonding with Eddie Brock.
- Jagged Mouth: When it causes Peter to lash out at Aunt May, it forms a serrated mouth. In all subsequent appearances, it has it on full-time often alongside More Teeth than the Osmond Family.
- Leitmotif: Accompanied by a distorted, slightly off-key version of Spider-Man's theme.
- Mythology Gag: Venom's appearance when it bonds to Flash Thompson matches his unique monstrous Venom form from the comics.
- Power Echoes: This series marks the first time effects are added to Peter's voice when he wears the symbiote as a costume instead of just limiting it to Venom.
- Significant Double Casting: The episode where he debuts, "Venom", has his voice actor also voicing Flash Thompson. That's because Flash ends up being taken over by him. At least it's only for that episode.
- Spot the Imposter: A villainous example in "Superior" where, while seeking revenge on the web-slinger for rejecting it, the symbiote clashes with Superior Spider-Man at Midtown High; its new ability to interface with people's memories leads it to realize that it had not been fighting Peter Parker, but Otto Octavius, who it dismisses as a fraud.
- Suddenly Voiced: He has his first speaking role in "Dead Man's Party", when he's bonded to Eddie Brock, whereas he didn't speak in the first season.
- The Symbiote: It is a black, amorphous entity that bonds to a host and augments them, at the cost of turning them into a vicious monster.
- Took a Level in Badass: After having been experimented on, it now has the ability to communicate with its host, and has a much higher resistance toward loud noises, previously the symbiote's most easily exploitable weakness.
- He's revealed to have taken another one in "Superior". Not only does he have much more control over his body, he also doesn't need a host. In addition, he can forcibly bond himself to others in order to use their memories. He's effectively an Eldritch Abomination at this point.
- The Voiceless: Unlike most versions, when attached to a host, such as Flash Thompson, Venom doesn't speak and only hisses and roars. This is eventually averted in "Dead Man's Party", where he speaks due to being attached on Eddie Brock.
- Yandere: If he wasn't before, he is definitely this by "Superior". When he confronts Peter, he sounds very much like a jilted lover.
- You Have Outlived Your Usefulness: A non-lethal variation. As soon as Venom has evolved past needing a host, it abandons Eddie Brock and later Curt Connors after it bonds to him to help it escape from Horizon High.
- Voiced by: Jim Cummings
A bitter ex-employee of Tony Stark.
- Crippling Overspecialization: After taking a key part of Stark's armor, he takes control of all the technology at the Expo he's currently in. He's eventually taken down when Spider-Man puts on the V-252, which Ghost can't control due to it being organic, and quickly loses the fight.
- Evil Sounds Deep: This comes with the territory for any villain that Jim Cummings voices.
Kraven the Hunter
- Voiced by: Troy Baker
The host and star of "Kraven's Amazing Hunt", who is hired by Norman to hunt down Spider-Man.
- Adaptational Comic Relief: While still an adept hunter, he's fond of constantly plugging his show and at one point gives Peter an autograph.
- Artificial Limbs: He has a robotic right arm, unlike other versions of himself.
- Broken Pedestal: Played for comedy; Miles used to be a big fan of his show, and is understandably disappointed when Kraven decides to go after them.
- Composite Character: He's based off of his initial appearance in the Ultimate Spider-Man comics, having a reality TV show, but has the combat proficiency of the 616 counterpart. His appearance also evokes how his second son Alyosha looked when he fought The Punisher.
- Dreadlock Warrior: He has rather Tarzan-like hair as befitting a skilled big game hunter.
- Egomaniac Hunter: Very egomaniacal.
- Eye Scream: He has an unexplained scar over his left eye, possibly an occupational hazard.
- Honor Before Reason: After being humiliated by Peter and Miles, he decides to outright try and kill them, and almost passes them up on saving his life on the basis he failed, and that he deserved to die.
- Large Ham: One of the most Laughably Evil villains on the show due to how theatrical he is, unable to resist talking to the camera and advertising products from his show at any given moment. Troy Baker is put to good use here.
- No Celebrities Were Harmed: He bears a striking resemblance towards Danny Trejo.
- Non-Standard Character Design: He's got a slightly more exaggerated, cartoonish appearance than the rest of the cast, most notably his sharp teeth.
- Worthy Opponent: In "Spider-Island Part 3", Peter gives him such a good fight that, after losing, Kraven gives him an autographed photo.
Blizzard (Randall Macklin)
- Voiced by: Trevor Devall
A petty thug who gets an ice gauntlet Peter and Harry created, and becomes a supervillain.
- Adapted Out: The first two Blizzards, Gregor Shapanka and Donald Gill, are unmentioned, making it appear that Randall is the first bearer of the identity in this continuity.
- An Ice Person: The ice gauntlet he gets allows him to create a field of ice around himself, make an icy Powered Armor, and also freeze over New York.
- From Nobody to Nightmare: Becomes a lot more dangerous once he starts getting the hang of the gaunlet's abilities.
- Kick the Son of a Bitch: Given that Hammerhead insisted on treating him like a regular mook, after giving Randall the idea behind becoming a supervillain, along with Hammerhead's already previously seen emotional manipulation of Keemia, it's hard to not see Randall's actions as at least somewhat karmic.
- Mundane Utility: Inverted. That gauntlet he stole from Peter and Harry? It was created for the purpose of refrigerating food, but became unexpectedly powerful after a gem was put in it, and Randall decided to use it to freeze over New York instead.
- No Ontological Inertia: The gauntlet alters his body as he uses it, with the right side of his hair being snow white, an icicle growing out of his shoulder, and having clearly blue veins, but having it removed immediately changes him back.
- Not Wearing Tights: He did not bother wearing a costume after becoming a Supervillain.
- Rogues Gallery Transplant: Blizzard is traditionally an Iron Man villain, but the character's spotlight episode doesn't feature Iron Man in any way, and also ties his origin story to an invention Peter and Harry created. The one used in this series, Randall Macklin, never even tangled with Spider-Man in the first place in the comics.
Absorbing Man (Carl "Crusher" Creel)
- Voiced by: Gregg Berger
A thug working for Hammerhead who can mimic the properties of anything he touches.
- Alliterative Name: Carl Creel.
- Assimilation Backfire: He falls victim to this twice. First he absorbs Spider-Man's webbing to smother him and gets a face full of web dissolvent causing him pain, then he absorbs lithium (which reacts badly to water) and when Spider-Man tricks him into touching water he starts buddling then dissolves to liquid.
- Dumb Muscle: He is pretty stupid, seeing as he didn't know some of the stuff Hammerhead was talking about and went onto Screwball's site to complain about Spidey while around Hammerhead.
- Epic Flail: As is tradition for Absorbing Man, he carries a wrecking ball and the chain it's attached to use as a weapon.
- Power Copying: He can mimic the properties of any substances he touches.
- Rogues Gallery Transplant: Usually a foe of Thor and the Hulk, here, he's fighting Spider-Man and encounters Hawkeye at one point.
- Sizeshifter: He can grow as well.
Crimson Dynamo (Galina Nemirovskyk)
- Composite Character: This version of the Crimson Dynamo's armor seems to be based on the Dmitri Bukharin armor, only piloted by Galina Nemirovsky.
- The Faceless: She is never shown outside her armor.
- Powered Armor
- Rogues Gallery Transplant: Usually an Iron Man villain, this character is fighting Spider-Man. Justified since the series features Iron Man.
- Samus Is a Girl: Due to the shape and size of her armor, Spidey mistakes her for a man in their first encounter.
- Unskilled, but Strong: She stole the armor, and Octavius hypothesizes that she only knows how the suit works at least at its most basic, but the suit is still powerful enough to knock around Spider-Man and Octavius.
Lizard (Curtis "Curt" Connors)
A scientist at Osborn Academy trying to find a way to regrow his arm.
- Adaptation Dye-Job: In the comics and other media, Curt is usually depicted with short, brown hair. Here, his hair is blonde and long.
- Adaptational Villainy: Downplayed. He's the same Tragic Villain as in other incarnations, but works for Oscorp, a company that is distinctly established throughout the series to be very shady. It's less downplayed in his return in season 3, where he's willingly acting as The Mole for a yet to be revealed individual and in just the first episode unleashes the Technovore and frames a student just to confirm some suspicions that Max has been continuing to experiment on the Venom symbiote. Admittedly, it's because he mistakenly believes Max's experiments are malicious in nature and wants to expose them, but other incarnations wouldn't usually stoop to such lows in order to do so.
- Alliterative Name: Curt Connors.
- Attack of the 50-Foot Whatever: Otto's "cure" ends up enlarging him into a giant Godzilla-like monster.
- The Bus Came Back: After his singular appearance in the first season, he returns at the beginning of Season 3 as a new instructor at Horizon High.
- Demoted to Extra: In most adaptations, Curt is Peter Parker's teacher, and often acts as Spidey's scientific advisor. This version has no such connections even after he becomes a new instructor at Horizon.
- Healing Factor: He is able to re-grow his tail.
- Perma-Stubble: He sports a prominent five-o-clock shadow.
- Professor Guinea Pig: Subverted; when introduced, he initially is presented as being this just like in the comics, but this is later revealed to be a lie; Norman Obsorn was the one who injected him with the lizard formula and used it to blackmail him.
- Tragic Villain: As usual, though with a twist. This version is introduced as having the same origin than usual, but it's eventually revealed Norman was the one who willingly turned him into a lizard, then used the promise of a perfected formula to regrow his arm to blackmail him. Connors still is blatantly not comfortable with the situation, though, and begs Norman to stop. And then he becomes another unwilling host for Venom at the start of Season 3, though he's slightly less sympathetic by that point due to the questionable actions he took beforehand to confirm to himself Max's "corruption".
- Swiss Army Weapon: His prosthetic arm can hack into control panels, which he uses to infiltrate Max's secret lab where he's creating the artificial symbiote.
Tinkerer (Phineas Mason)
- Voiced by: Aaron Abrams
- Adaptational Badass: Downplayed; His comics counterpart preferred not to fight heroes face to face. This version is more willing to fight Spidey directly.
- Evil Is Hammy
- Freudian Excuse: The reason he has been sabotaging the baseball game is because he was tired of his team losing.
- Harmless Villain: Spider-Man defeats him in a matter of minutes and doesn't take him remotely seriously.
Lady Octopus (Carolyn Trainer)
- Voiced by: Kari Wahlgren
- Adaptational Heroism: Lady Octopus is typically depicted as Octavius's Stalker with a Crush to the bitter end, mercenary at best, and a full on villain at worst. Here, Carolyn sees him as a Broken Pedestal after her debut episode and undergoes a Mistreatment-Induced Betrayal HeelFace Turn, giving up villainy in the process.
- Adorkable: She may be crazy, but it's actually cute in a twisted way how she cares for and shows affection for her robots. There's also her general awkwardness in trying a bit too hard to be good at villainy.
- Beware the Silly Ones: Her crush on Octavius is played for comedy, she behaves like a stereotypical teenager, and due to her inexperience as a villain she ends up making rookie mistakes such as confusing her schedules. Still, she is competent enough to give Spider-Man a hard time, and almost fights toe-to-toe with a psychic-powered Dr Octopus after turning on him..
- Broken Pedestal: She is infuriated when she finds out she was just another pawn in Octavius's plan, and promptly turns on him.
- Even Evil Has Standards: While she's definitely crazy, she still has standards, and expects to be seen as an equal. As such, when Doctor Octopus reveals he was using her, she rightfully turns on him.
- HeelFace Turn: Gives up villainy after she finds out she was being used by Otto.
- Mistreatment-Induced Betrayal: She helps Spider-Man save Miles, Gwen, and Anya when Doc Ock betrays her.
- Stalker with a Crush: She hacked the prison's computer system just so she can be with Otto Octavius, clearly showing the possessive love she has for him and his mind. She loses her feelings for him upon being betrayed.
- Younger and Hipper: She's a teenager around Doc Ock's age in this, making her crush on him squicky only to the characters.
The Wake Riders
- Voiced by: Ogie Banks (Barkley Blitz), Audrey Wasilewski, Zack Shada
A team of stunt performers who use their tech to perform air shows anywhere. In reality, they are actually thieves.
- Bitch in Sheep's Clothing: They present themselves as stunt performers in it simply for the thrill. Miles is an avid fan of theirs, and they grow on Spidey after incorporating him in a show. They're actually remorseless thieves and Loony Fans of the Vulture.
- Broken Pedestal: After learning the truth about them, Miles loses any respect he had for the Wake Riders.
- Canon Foreigner: They were created exclusively for the cartoon. None of them exist in the comics.
- Evil All Along: As Spider-Man finds out later on, the Wake Riders are a bunch of thieves that have been using him to cover up their track.
- Manipulative Bastard: Their manager, Gabby Flenkman, is a very manipulative woman, who is able to convince Jameson that Spider-Man framed the Wake Riders, which adds more fuel to his dislike of the wall-crawler.
- The Smurfette Principle: Only one of the Wake Riders is female.
A high-tech criminal who tries to rob an armored truck.
- Animal-Themed Superbeing: A beetle, obviously.
- Bit Part Bad Guy: He only shows up at the beginning of "Dead Man's Party".
- Mythology Gag: This version of Beetle is based on the Abner Jenkins incarnation.
- Powered Armor: Like most incarnations of the character, he's just a regular human fighting in a high-tech armor.
- Small Role, Big Impact: He only shows up at the beginning of "Dead Man's Party", but it helps set up Eddie Brock's eventual turn to darkness.
- Would Hit a Girl: In "The Living Brain", he throws Chief Wantanabe against a wall during his fight with her and Spider-Man.
Hippo and Panda-Mania
- Voiced by: Zach Shada (Hippo) and Teala Dunn (Panda-Mania)
A criminal duo that try to rob a bank, only to be foiled by Spider-Man.
- Artificial Limbs: Hippo has a robotic left leg.
- Harmless Villain: While the two manage to rob a bank as well as give Spider-Man a run for his money, they're woefully unprepared for his persistence, and otherwise don't pose a significant threat to him or even New York. They seem to realize this themselves, and are the first mercenaries to bow out of the hunt for Spider-Man and go visit a wax museum instead.
- Know When to Fold 'Em: They go to New York due to being hired by a mysterious benefactor, along with a number of other mercenaries. Once they find out that said mysterious benefactor wants them to go after Spider-Man, who had gave them a significant amount of trouble hours before, they immediately decide to leave right then and there.
- Panda-ing to the Audience: Panda-Mania wears a panda costume.
- Voiced by: Ryan Blanely
A high-tech criminal who has control over nanites.
- Adaptational Villainy: In the comics, Overdrive was a superhero fanboy who had planned to become a hero at some point after pulling a HeelFace Turn. Here, there's no indication this is the case and he seems like just another supervillain.
- But for Me, It Was Tuesday: As he's being taken in by the police, who also give Spider-Man a speeding ticket, he casually tells him "Yeah, you get used to 'em."
- Only in It for the Money: Like many other villains, he's mostly there for the bounty on Spider-Man's head.
Spot (Johnathan Ohnn)
- Voiced by: Crispin Freeman
Mysterio (Quentin Beck)
- Voiced by: Crispin Freeman
- Evil Is Hammy: About per the course for Mysterio, he's over the top. Additionally, he is voiced by Crispin Freeman, who can be a Large Ham in his own roles.
- Hoist by His Own Petard: Spider-Man defeats him by reprogramming a Robot Duplicate he used against him, thus tricking him into thinking he defeated the actual Spider-Man and causing him to drop the illusion.
- Master of Illusion: His specialty is to make people see what he wants them to see.
- Voiced by: Booboo Stewart
A Halloween-obsessed supervillain who is after the bounty placed on Spider-Man's head.
- Abnormal Ammo: As noted below, he uses candies as munitions. Even other supervillain Beetle is utterly confused about why someone would make such a weapon.
- Axe-Crazy: To quote Spider-Man, "this guy is crazy even by supervillain standards".
- Evil Is Hammy
- Exact Words: When asking "Trick or Treat" to Spider-Man, Spidey complains there is no point in answering, since all he ever gives is tricks. Jack O'Lantern, however, assures him it'll be a treat this time - and then proceeds to shoot him with a gun that fires candies.
- Laughing Mad: Constantly laughing, and quite insane.
- Psychopathic Manchild: Despite being apparently a full-grown man, he has a childish obsession with Halloween, constantly throwing puns based on it and acting gleeful when asked "Trick or treat".
- Voiced by: Nathaniel J. Potvin
- Anti-Villain: He is only a supervillain out of genuine need for money to save the life of his brother. Moeover, he has a genuine code of honor, which causes him to abandon his hunt for Spider-Man when the latter saves his life. This makes all the easier for him to make a HeelFace Turn.
- Badass Normal: He is a regular human who relies entirely on a high-tech gear, yet can give trouble even to the super-powered Spider-Man.
- Brains and Brawn: With him being the Brawn; by his own admission, he is no scientist, just a muscle; his gear was developped by his brother.
- Composite Character: It's Hobie Brown, but the costume is based on the one Aaron Davis wears.
- HeelFace Turn: After Spider-Man helped him save his brother from Silvermane, he gives up supervillainy and starts considering a career as a superhero.
- I Owe You My Life: Decides to let Spider-Man go once the latter saves him from a malfunction of his own gear.
- Logical Weakness: He doesn't build his own gear, his brother does; as a result, when his brother is held prisoner by Silvermane, he is unable to fix his equipment, leaving him vulnerable to the slightest malfunction.
- Noble Demon: Despite being a supervillain, he does have something of a code; namely, he refuses to take down Spider-Man after the latter risked his life to save his.
- Nothing Personal: Said word to word to Spider-Man; to him, catching Spidey is a job, nothing more.
Silvermane (Silvio Manfredi)
- Voiced by: Nolan North
A crime boss who is holding Prowler's brother hostage.
- Badass in a Nice Suit: At least until he ditches it for his armor.
- The Don: Lampshaded; Spider-Man points out how he follows the cliche to the letter.
- Evil Old Folks: An elder mafiosi crime boss.
- Powered Armor: He wears a suit of armor that shoots electricity.
- Psycho Electro: His Powered Armor is able to shoot out electric currents while his eyes glow.
Electro (Francine Frye)
- Voiced by: Daisy Lightfoot
- Adaptational Wimp: Unlike either of the comic incarnations of the character who have superpowers, this one has to rely on tech with a low budget, which she frequently ends up having to fix mid-fight against Spider-Man. When she ends up stealing an amplifier Peter created, she's much more in-line with how powerful the comics character can be, with her later appearance in prison suggesting that she's become a metahuman after her first appearance.
- Age Lift: She's a teenager in this incarnation as opposed to an adult woman.
- Alliterative Name: Francine Frye.
- Axe-Crazy: Not quite as insane as Jack O'Lantern, but she is quite gleeful about going on rampage and blowing everything up with lightning.
- Composite Character: She's the second Electro, Francine Frye, but has an Energy Being form similar to the Ultimate incarnation of Max Dillon. Her being black might also be from The Amazing Spider Man 2's version of Dillon. She's also an Engineer like Dillon.
- Electric Black Guy: Or rather girl.
- Psycho Electro: In case the name wasn't a hint, she has power over electricity, and is quite insane and destructive.
- Race Lift: Francine Frye is Caucasian in the comics, but is African-American in this series.
- Shout-Out: One of her attacks, putting her hand against the ground to form a sort of blade made of lightning, resembles the Raikiri technique.
- Took a Level in Badass: Using Peter's amplifier turns her into a full-blown metahuman on par with her comic book counterpart and a genuine threat.
- Voiced by: Patton Oswalt
A master of disguise and the (alleged) mastermind behind the bounty on Spider-Man's head who he frames for robbing a bank.
- Arc Villain: Subverted. He appeared to be the Big Bad in the "Bring on the Bad Guys" arc, but it's revealed that he was merely The Dragon to Otto Octavius, the real mastermind.
- The Dragon: To Otto Octavius for the "Bring on the Bad Guys" arc.
- Foreshadowing: He briefly shows up as Captain America to lure Spider-Man into Mysterio's lair, before being introduced under his proper identity.
- Master of Disguise: As usual in all versions, thanks to a special device built in his mask.
- Mythology Gag: His holographic disguises serve as an homage to his 1994 animated counterpart, only that he uses his mask instead of his belt.
- Red Herring: Invoked; he pretends to be the actual mastermind behind the bounty on Spider-Man's head, thus allowing the actual mastermind to catch Peter offguard once he is imprisoned.
- Undying Loyalty: Despite being used as a Red Herring by his hirer in a plan which caused him to end up imprisoned, he actually seems rather happy that he was able to help.
- Voiced by: Jonathan Freeman
- Composite Character: This depiction is an amalgamation of his original counterpart and his son Tyler Stone.
Mr. Negative (Martin Li)
- Voiced by: Eric Bauza
The superpowered leader of a criminal gang called the Inner Demons.
- Deliberately Monochrome: As a side effect of his powers, his skin is colored gray while his hair is white as well as his suit.
- Evil vs. Evil: He is Molten Man's rival for territory.
- Sharp-Dressed Man: Mr. Negative wears a suit with a tasteful waistcoat. Combined with his eloquent speech, it sets him apart as more refined than Molten Man.
- Sword Beam: Mr. Negative can shoot energy from his sword. He thinks it's pretty awesome.
- Voiced by: Imari Williams
A magma-powered superbeing and gang leader.
Regent (Augustus Roman)
- Voiced by: Imari Williams
A mysterious villain who has the powers of other supervillains held in the Cellar, a maximum security supervillain prison.
- Adaptational Villainy: In the comics, Regent did what he did because he lost his family in the crossfire of a superhero/supervillain battle, which incited deep resentment in him, and drove him to become the only hero the world would need. Here, he hates heroes because his father was a criminal (a fact he is well aware of) who was arrested and locked up thanks to the Avengers.
- All Your Powers Combined: His armor utilizes the powers of people imprisoned in the Cellar: Mr. Negative's darkforce manipulation, Molten Man's pyrokinesis, Electro's electricity, Sandgirl's sand manipulation, Cloak's portal creation, Dagger's hard light weapons, Frostbite's cryokinesis, Whirlwind's aerokinesis, and Paradox's green Hand Blasts.
- Arc Villain: For the "Brand New Day" and "The Cellar" episodes.
- Bad Boss: He frames his assistant Shannon by placing the plans for the Regent suit in the database using her username, planning to use her to take the fall after taking care of the Avengers and Spider-Man.
- Bitch in Sheep's Clothing: In his introduction, he's extremely friendly and speaks with a clam cadence more suited to a yoga instructor than a prison warden. He's significantly more hammy when he drops the act.
- Broken Pedestal: Chief Watanabe always thought of him as a father figure. However, once the truth comes out about who he is, she loses all respect for him.
- Evil All Along: He is revealed to be the Arc Villain for the episodes he's featured in.
Slyde (Jalome Beacher)
- Voiced by: Phil La Marr
A slippery supervillain who plans on creating a virus to wipe out all of New York.
- Freudian Excuse: He was once an employee at Beemont Chemicals until he came to believe that Allan Beemont had stolen his ideas, and so he was fired.
A robotic creature that wreaks havoc in Horizon High in the season 3 premiere.
- Rogues Gallery Transplant: While a foe of Iron Man, this version fights Spider-Man.
- Voiced by: Leonard Roberts
An adversary of Doctor Strange who works with A.I.M. to try and capture Groot.
The headmaster and founder of Horizon High, who recognizes Peter's potential and recruits him in his school.
- Alliterative Name: Max Modell.
- Chronic Backstabbing Disorder: Inverted. He tends to make a lot of enemies with the people he makes ties with, and then they turn on him.
- Fatal Flaw: Max's biggest problem is that once he gets an idea in his head, he will not be convinced he might be wrong until it blows up in his face. Norman has exploited this a few times to further his own agenda by making both Harry and Otto look bad in Max's eyes and start creating tension between them. Making things worse is that, while he has enough grace to admit when he has been wrong, he tends to swing to the other side of the spectrum from his initial choices rather than learn that his doubling down might not be the best idea.
- Freudian Excuse: It's revealed in "Web of Venom", that early in his life he was caught in an accident and someone got hurt helping him. This trauma stuck with him and became the reason for his obsession with believing V-252 could people, so no one would have to get hurt again.
- Glory Hound: He's accused of being this by several characters, such as Norman, and "Stark Expo" shows a grain of truth to this sentiment; he ignores Peter's concerns that the V-252 is alive, because he's too concerned with proving it can be on the periodic table. After Spider-Man uses the suit, proving once and for all that it's a symbiotic organism, he's quick to admit he got too obsessed with it, to the point of ignoring his students.
- Ink-Suit Actor: He looks like Fred Tatasciore, with a beard.
- The Mentor: To Peter, being the one who acknowledges his potential and takes him to Horizon High.
- Out of Focus: He stops showing up as frequently after the Venom arc in Season 1, with more focus going to Peter and his relationship with Harry and the other Spider-Heroes.
- Reasonable Authority Figure: The main authority at Horizon High, and overall a rather reasonable and well-meaning kind of guy.
- Red Herring: Peter believes he has become the host for the V-252. Turns out he's wrong.
- Secret Keeper: He pieced together Peter's identity as Spider-Man during his fight with the Goblin King, which he reveals to him in "Web of Venom" with the assurance that he'll keep it a secret.
Rhino (Aleksei Sytsevich)
- Voiced by: Matthew Mercer
- Adaptation Dye-Job: Aleksei is usually a brunet in the comics and most versions before becoming the Rhino. This version of him is a redhead.
- Adaptational Heroism: Pre-Rhino, he's a pretty fun guy to be around, and is rather nice to the other Horizon students. He also didn't willingly become the Rhino. In the comics, Aleksei was already a thug and volunteered to become the Rhino.
- Adaptational Intelligence: Even pre-Rhino, the comics Aleksei Sytsevich was a Dumb Muscle thug. This version is a Teen Genius before he's the Rhino.
- Adaptational Wimp: This version of Rhino is nowhere near as tough as past incarnations of the character, being little more than a humanoid brute with the mind of an animal. Against Spider-Man, he doesn't even get in a single hit in their short fight.
- Baleful Polymorph: Was forced to become the Rhino.
- Brainwashed and Crazy: Becomes a member of the Sinister Five with a mind control device in his neck.
- Comic Book Movies Dont Use Code Names: An animated example. Aleksei is never referred to as the Rhino at all.
- In Name Only: Just like Herman/Shocker and Clayton/Clash in this series, this version of the Rhino has nothing in common with his comic book counterpart and most versions outside of his name, powers, and nationality.
- Never Trust a Trailer: This Rhino, much like his Ultimate Spider-Man counterpart, is a teenager mutated into a more Rhino-like appearance. However, a poster to promote the show◊ features the traditional thug in a costume Rhino.
- Nice Guy: He's pretty amicable to the other students at Horizon.
- Younger and Hipper: Like with Doctor Octopus, the Shocker, and Alistair Smythe, the Rhino is around Peter's age.
- Voiced by: Scott Menville
Eugene "Flash" Thompson
- Voiced by: Ben Diskin
- Abled in the Adaptation: Much like Ultimate Spider-Man, Flash still use the full use of his legs when he bonds to the Venom symbiote, unlike the Flash of the comics, who was an adult soldier who lost the use of his legs in battle before becoming Agent Venom.
- Adaptational Intelligence: Heavily downplayed compared to the rest of the cast, but this version of Flash is at least interested enough in science to build a decent soda volcano and enter a science expo with it, whereas the comics version of Flash would have never engaged in any activity of the sort.
- Adaptational Nice Guy: Played with, Flash is most famous for being The Bully to Peter, and rarely does he grow out of this phase in adaptations. Here, Flash has the same past of bullying Peter but by the time the show takes place he has eased off.
- Boisterous Bruiser: Somewhat downplayed compared to his previous Ultimate Spider-Man incarnation, but only because this Flash has considerably lesser screen-time at this point then his prior version did, especially once his prior version became Agent Venom. He still manages to showcase this trope in his one focus episode so far, both during the temporary point when he was bonded to the Venom Symbiote and the climax after during the football game. Also, fact that he's mellowed from explicitly bullying Peter outside keeping his "street cred", he's more receptive to being this from the onset compared to his prior incarnation who had to grow into being this.
- Composite Character: He is the original Venom instead of Eddie Brock. Although Flash himself did eventually become Venom in the comics. His interest is science (and lack of actually being good at it) might've also come from his Marvel Cinematic Universe counterpart. Also, he sports a similar buzzcut to the one sported by his The Amazing Spider-Man Series incarnation.
- Dark-Skinned Blond: He has blond hair, but it is unknown if his skin is tan.
- Disqualification-Induced Victory: Parodied; his soda volcano ends up winning Stark Expo despite being by far the least impressive project, solely because Ghost happened to wreck all the other inventions.
- Dumb Jock: This version of the character plays up the fact he isn't particularly smart. Granted, the fact he is a normal teenager in a show where the large majority of the cast are super-geniuses doesn't help.
- Jerk Jock: Downplayed; he is portrayed as not particularly smart and a bit cocky, but his bullying days are behind him.
- Jerk with a Heart of Gold: He still occasionally picks on Peter, at one point saying he has to keep up appearances, but a lot of it is mostly lighthearted ribbing, and he's quick to defend Peter after two people try to pick on him after he's finished tutoring Flash, because it saved Midtown.
- Mythology Gag: Venom using him as a host seems to be a reference to his Agent Venom role in both the previous show and the comics.
- Odd Friendship: He's a slightly rough jock and kind of a meathead, but is on amicable terms with the nice, nerdy Peter Parker, even before he started tutoring Flash.
- Race Lift: Possibly; he was white in the comic, while he is Ambiguously Brown here.
- Reformed Bully: Flash by the time the story takes place has given up his former bullying ways. Even more so in "Venom"—he started out being hard on Peter due to him being a nerd he can solicit help from, but Peter's tutoring saved his life, so he stood up for him in the end.
- Took a Level in Kindness: Although Flash has nowadays has stopped tormenting Peter, he still gave him a hard time when he asked him to tutor him in chemistry, as the only thing that mattered to him was being able to play in the football game. Ultimately, what he learned from Peter did save his life and the school, and he has been grateful to him since.
- Wisdom from the Gutter: Flash of all people manages to get Otto (in Peter's body) to reconsider his actions as the Superior Spider-Man by voicing his disapproval for the new Spider-Man's approach to criminals and telling him that being a hero is more than just keeping crime rates down.
Screwball (Liz Allan)
- Voiced by: Natalie Lander
- Adaptation Dye-Job: Liz is blonde in the comics. This iteration of her is a brunette.
- Adaptational Heroism: Screwball is a villain in the original comic, but here Screwball is introduced as an anti-hero before gradually becoming a full hero over the course of her episode.
- ...But He Sounds Handsome: Being directly asked about Screwball always gets a compliment for Screwball.
- Composite Character: Screwball and Liz are separate characters in the comics, but here, Screwball is Liz's alter ego.
- For Want of a Nail: She almost became the one with spider powers. Had she not have realized that she was left-handed at the last second before switching places with Peter in Origins, the radioactive spider would've landed on her hand and bitten her instead of Peter.
- I Just Want to Be Special: Liz is popular in Midtown, had one of the highest grades and is a student body president. But after the arrival of schools such as Horizon High and Osborn Academy where students smarter than she attended, she realized that her achievements were no longer enough.
- Jerk with a Heart of Gold: She's not the best person ever but she does ultimately want to do good.
- Leitmotif: A clownish, out-of-sync, theme plays whenever she performs her pranks.
- Motive Decay: She did her Screwball shtick to prank major corporations that were doing bad things, but upon finding out that pranking Spider-Man gave her a lot of views, she decides to keep making videos about Spider-Man, which she stops after she realizes it put everyone in trouble.
- Ordinary High-School Student: Deconstructed; she used to be a pretty important student in Midtown High, but the arrival of Horizon High and Osborn creating his own school brought multiple Teen Geniuses in the spotlight, leaving her Overshadowed by Awesome. Becoming Screwball partially was a way to make her special again, though she still was trying to do good before Motive Decay kicked in.
- Science Hero: Keeping with the theme of the show, most of her equipment is advanced tech. How she got them is left in the air.
- Troll: Her Screwball persona is essentially this.
- Voiced by: Felicia Day
- Adaptation Origin Connection: She and Peter already know each other, and Peter turns his head on Flash pointing out that it's her underneath the Tiger mascot. * Ascended Extra: After only making a brief cameo in a costume during season 1, she finally appears in person in the Maximum Venom season.
- Badass Bystander: She leads the crowd to blow the horns to create a sonic attack against Venom, on Spider-Man's orders.
- Mythology Gag: In her earliest appearance in Spider-Man, MJ was The Faceless and kept off-screen until her famous and iconic introduction. Also, she appears in a tiger costume, her iconic nickname for Peter was "Tiger".
- Voiced by: Zeno Robinson
- Alliterative Name: Randy Robertson.
- Black and Nerdy: In contrast to his comic book counterpart and other versions. The twist is that in a show full of science geeks, he's a journalism nerd.
- Expy: Visually of Miles Morales' supporting character, Judge.
- Ordinary High-School Student: He isn't a super-genius and is just a normal boy.
- Small Role, Big Impact: He only appears for two minutes in the season 2 premiere, but it's his helping Peter get a job at the Daily Bugle that kicks off many events for season 2.
Anna Maria Marconi
- Voiced by: Katrina Kemp
- Age Lift: A minor one. She was a university student in the comics, but she's an assistant teacher here.
- All of the Other Reindeer: It's not shown in the present, but she alludes to having been bullied during her time as a student due to her dwarfism.
- Birds of a Feather: With Otto Octavius. Both of them are ambitious and intellectually gifted people who were ostracized for their physical statures. This is partly what draws Otto to her.
- Morality Pet: She's probably the first person that Otto treats with upmost kindness and respect, and ultimately the one to push him towards cementing his HeelFace Turn.
- Secret Keeper: She finds out that Peter is Spider-Man at the end of the "Superior Spider-Man" arc once the Venom symbiote attacks.
- Ship Tease: Otto notes that his heart actually skips a beat around her. She also seems pretty drawn towards him even after finding out about his past as a supervillain.
- Shorter Means Smarter: She has dwarfism, was smart enough to have a shot at being enrolled into Horizon High, and is an assistant chemistry teacher.
- Voice with an Internet Connection: She joins Otto in this role for Peter and the other Spiders when they need to multitask.
Shocker (Herman Schultz)
- Voiced by: Cameron Boyce
An aspiring inventor who applies for Osborn University alongside his friend Cole. He invented a set of vibro-shock gauntlets named the Shockers.
- Adaptation Origin Connection: He's the best friend of Clayton Cole/Clash in this show.
- Adaptational Heroism: Most versions of Shocker are criminals, this version of Herman Schultz is just a high school student and he gives Spider-Man advice by telling the weakness of Clayton's suit when the Jackal took their gear.
- Comic-Book Movies Don't Use Codenames: An animated example. Herman is never referred to as Shocker. However, he does use the code name for his invention.
- In Name Only: Has nothing in common with his comic book counterpart outside of his name and invention.
- Younger and Hipper: Like Alistair Smythe and Doctor Octopus, the Shocker is around Peter's age and hence a teenager.
Ultimate Spider-Slayer (Alistair Smythe)
- Voiced by: Jason Spisak
- Adaptation Dye-Job: Alistair Smythe is usually portrayed with having brown hair. Here, it's ginger.
- Actually Pretty Funny: When Harry snarks that Spencer Smythe kicking Peter to the moon isn't scientifically possible, even Alistair laughs.
- Berserk Button: He doesn't like it when someone insults his dad.
- Brainwashed and Crazy: Becomes a member of the Sinister Five with a mind control device in his neck.
- Composite Character: Temporarily dons the Vulture suit to be the second Vulture.
- Powered Armor: He dons the Vulture's armor to attack Horizon High and later an Oscorp Spider Slayer mecha suit while fighting his dad. He later dons a suit of Spider Slayer armor resembling the Crimson Dynamo's when he's a member of the Oz Commandos.
- The Speechless: As the Spider-Slayer, Alistair doesn't speak. Justified since he is under mind control courtesy of Doctor Octopus.
Man-Wolf (John Jameson)
- Adaptation Dye-Job: Man-Wolf is now depicted with black fur, rather than white fur just like his comic book counterpart and other versions.
- Adaptational Intelligence: While the John Jameson on the comics wasn't an idiot, he also wasn't a scientist (in fact, his Man-Wolf form was originally mystic in origin). Here, he's a Teen Genius and being Man-Wolf is a result of his work.
- Alliterative Name: John Jameson.
- My God, What Have I Done?: His reaction to turning into the Man-Wolf and creating similar wolf creatures while in that state.
- Our Werewolves Are Different: He is turned into a werewolf-like creature after being exposed to a mysterious lunar crystal.
- Younger and Hipper: John is a teenager as opposed to an adult like his comic book counterpart and other versions.
Steel Spider (Oliver "Ollie" Osnick)
- Adorkable: His admiration of Otto Octavius is quite endearing. Too bad he's brainwashed by him.
- Alliterative Name: Ollie Osnick.
- Brainwashed and Crazy: He becomes an Unwitting Pawn in the Sinister Five with a mind control device implanted in his neck.
- The Speechless: Averted. Even when brainwashed, Ollie is the only member of the Sinister Five who speaks.
J. Jonah Jameson
- Voiced by: Bob Joles
- Adaptation Personality Change: A minor one, but in the comics, J.J. pretty much hated all masked superheroes with the exception of Captain America. Here, however, he's often calling the Avengers real heroes and his dislike only seems to be directed at Spider-Man, his reason being that the Avengers all made their identities public while Spider-Man didn't.
- Adaptational Jerkass: In the comics, Jameson suspected Peter had some kind of connection to Spider-Man due to him being the only one who could get good pictures of the webslinger, but he didn't hold it against. When Peter "confesses" that he and Spider-Man have a similar connection, Jameson fires him on the spot.
- Adaptational Nice Guy: Combined with Adaptation Personality Change, while he's not a saint by any means, he's still a somewhat more reasonable person than his comic book counterpart and most versions.
- After spending most of time ranting about how Spider-Man is a "menace" who needs to be locked up, "The Day Without Spider-Man" shows him complaining when Spider-Man is a no-show, claiming that he's "more of a menace" when he doesn't show up.
- Despite hating Spider-Man for not being a "real hero", when the Superior Spider-Man shows up at his office and makes his Anti-Hero ways clear, Jameson actually approves of it.
- Jerkass: As usual in most incarnations. This version takes it up a notch by flat-out bullying some of his employees and outright fires Peter beliving he's connected to Spider-Man without hearing the boy out.
- Jerk with a Heart of Gold: It's not too noticeable, but he has his moments. He's actually somewhat cordial with Peter when he proves his worth when getting good footage of Spider-Man, and is less prone to yelling at him than with Eddie Brock.
- Large Ham: He's not over the top for nothing.
- Lethally Stupid: On at least two occasions when he's been face to face with Spidey, he's attempted to make a "citizen's arrest", in spite of the fact that Spider-Man has Super Strength and could easily snap him in half if he were really the "menace" Jameson believes him to be.
- Mean Boss: At the end of the day, he will not consider the opinions of any of his employees, and he's definitely dismissive of Peter Parker speaking of Spider-Man in a good light. When Peter complains about how he's not being truthful, Jameson tells him to get on board or get out.
- Never My Fault: He refuses to admit that he had a part in Eddie's transformation into Venom, even though both Spider-Man and Venom rightfully tell him otherwise.
- No Indoor Voice: Communicates by shouting, both on TV and to his employees.
- Ungrateful Bastard: Just like Harry in season 1, he does not show appreciation towards Spider-Man for saving him from Venom and only bashes him further.
Edward "Eddie" Brock
- Voiced by: Ben Pronsky
- Adaptational Nice Guy: Here Eddie is just a down-on-his-luck and less-than-competent photographer that's forced to endure a lot of undeserved crap from J. Jonah Jameson and Peter, while having sympathetic reasons for doing so, is taking Eddie's job from him. This gives Eddie more understandable reasons to hate him compared to the original comics, where Brock wrote a series of sensationalist interviews with a supposed serial killer for weeks before actually going to the authorities and hates Spidey for catching the real serial killer and causing him to lose his reputation and job.
- Destined Bystander: His early appearances are unvoiced and he doesn't get much focus until Peter begins working at the Bugle and starts inadvertently scooping him on Spider-Man stories. Of course by this point, the symbiote has long been established.
- Early-Bird Cameo: He makes an unvoiced appearance early in Season 2, trying to record Spider-Man.
- I Just Want to Be Special: Implied. When the Symbiote begins to come off of him, he begs it not to leave because he doesn't want to be "nothing".
- Never My Fault: Downplayed compared to the original comics version; Eddie in this series does show an inability to acknowledge his own flaws, such as lacking any real talent as a photographer, being sleazy in his day-to-day maintenance and hygiene, or being something of an all-around creep. On the other hand, Eddie does have a dedicated work ethic and never commits any form of journalistic fraud. Meanwhile Peter, for all his understandable reasons for doing so, is using fraudulent pictures to take Eddie's job from him, and J Jonah Jameson does treat him like garbage in a way Eddie doesn't deserve. That being said, Eddie to some degree is aware of how others perceive him and mid-Villainous Breakdown admits he feels like nothing without the Symbiote.
- The Resenter: He hates Peter for stealing his job . This hatred allows the symbiote to sympathize with him and the two to bond.
- Stalker Without a Crush: Obviously stalks Spider-Man in an attempt to get good photos of him, but a far more disturbing case happens with Peter; after bonding with the symbiote, he breaks into Peter's room, and is found creepily searching through his personal goods and watching his family photos, commenting how he can see Peter's whole life has gone so well.
- Villain Has a Point: Eddie may not be as good a photographer as he thinks he is, but Peter is cheating him out of his job with glorified selfies, wittingly or not.
- Voiced by: Ernie Hudson
A pro-technocracy terrorist organization that seeks to control technology.
- Animal Motifs: Bees, as usual. It represents how they seek to turn the society into a hive-like structure with them as the Queen.
Scientist Supreme (Monica Rappaccini)
- Voiced by: Grey DeLisle
The headmistress of a boarding school called the Bilderberg Academy who has a sinister agenda for her students.
- Powered Armor: She wields a suit of mechanical armor while in battle.
- Voiced by: Charlie Adler
The newest creation of A.I.M., who finds himself at odds with Superior Spider-Man.
- Enemy Civil War: There are multiple, competing factions of Hydra. The ones we see are Arnim Zola's faction.
- Faceless Goons: Unlike some versions, who are Gas Mask Mooks, these soldiers do not wear gas masks.
- No Swastikas: Their links with the Nazis is not mentioned here.
- Plot-Irrelevant Villain: While they are the main antagonists of the second part of the "Spider-Island" event, they are not really relevant to the arc overall.
Crossbones (Brock Rumlow)
An agent of Hydra who is after a key to a Vibranium vault.
- Badass Normal: He is able to fight spider-powered heroes like Spider-Man and Spider-Gwen.
- The Dragon: To Arnim Zola.
- Empowered Badass Normal: He is Badass as mentioned before he ends up with spider powers.
- Evil Sounds Deep: Well, for a villain voiced by Fred Tatasciore, it comes with the territory.
- Power Echoes: A notable trait of his.
- Rogues Gallery Transplant: He's traditionally a Captain America villain, but is a recurring villain in this series.
- Voiced by: Mark Hamill
Leader of one faction of Hydra.
- Composite Character: His body is based off of his comics counterpart, but the numbers that form a face is from his digitalized incarnation in Captain America: The Winter Soldier
- Mythology Gag: His design, with numbers going down a screen with them forming a face, borrows from the computerized version of the character in Captain America: The Winter Soldier.
- Voiced by: April Stewart
The leader of the Wild Pack who steals a device called the Neuro Cortex for an unseen client.
- Adaptational Villainy: Silver Sable in the comics was both a foe and ally of Spider-Man, while she is a full villain in the show.
- Alliterative Name: Silver Sablinova.
- Badass Normal: She seems to be just a normal human with enhanced equipment, yet can fight decently against the super-powered Spider-Man.
- Light Is Not Good: Her white hair and light clothing is not much telling of her.
- Reality Ensues: Despite being a rather efficient Badass Normal, she still is a regular human with no apparent enhancement. As a result, when she fight the superhumanly agile, wall-crawling Spider-Man on the top of a flying jet, she struggles to even maintain her balance, while Spidey keeps clinging despite her best efforts.
Paladin (Paul Denning)
- Voiced by: Trevor Devall
A mercenary who pretends to rob Oscorp in order to study Spider-Man's moves.
- Awesomeness by Analysis: He is sent by the Wild Pack to fight Spider-Man specifically so they can study his moves and later use that knowledge to better fight him.
- Light Is Not Good: His code name is based off of a holy knight, but does not act the part.
- Non-Indicative Name: Mocked; Spider-Man points he neither looks nor behaves even remotely like a Paladin. He justifies by stating it merely is a codename.
Puma (Thomas Fireheart)
A cat-like mutate who is a member of the Wild Pack.
Battlestar (Lemar Hoskins)
A large member of the Wild Pack.
- Voiced by: Nancy Linari
- Adaptational Attractiveness: Similar to her counterpart in Ultimate Spider-Man, Aunt May looks much more youthful than how she looked in the comics.
- Amazingly Embarrassing Parents: Perhaps not all the time, just in relation to her dancing.
- Out of Focus: Doesn't actually appear all that much in this version, especially compared to Harry, Miles, Anya and Gwen; in fact, she's absent from the majority of episodes.
- Silver Fox: While it's become kind of a running gag since the first Ultimate comic, this version of Aunt May pretty much looks like a slightly aged-up version of most of the teenage female characters only with granny hair and slight eye wrinkles.
Benjamin "Ben" Parker
- Voiced by: Patton Oswalt
- Death by Origin Story: Naturally, being part of a Spider-Man series, his death is required to push Peter into using his great powers responsibly.
- Morality Chain: A bizarre example in that he isn't alive yet still plays the role. Otto admits that Peter's memories of Uncle Ben that he obtained during the "Freaky Friday" Flip are what are keeping him from slipping back into evil.
- Nice Guy: Flashbacks show him as being supportive of Peter.
- Out of Focus: Oddly enough, the shorts before the series didn't feature him with an onscreen presence in any way. While the final short covers his death due to Peter's indirect actions, he still doesn't show up at all barring a picture. The series circumvents this a bit by occasionally showing him in flashbacks, and he's mentioned every now and then.
- Posthumous Character: He's dead by the time the series properly starts, only occasionally showing up in flashbacks.
Clash (Clayton Cole)
Another aspiring inventor who applies for Osborn University alongside his friend Herman. He invented a suit that lets him absorb sounds and use them as sonic pulses.
- Adaptation Origin Connection: He's the best friend of Herman Schultz/Shocker in this show.
- Alliterative Name: Clayton Cole.
- Comic Book Movies Dont Use Code Names: An animated example. Just like with Herman in this series, Clayton is never referred to as Clash and he only uses the code name for his invention.
- In Name Only: Just like Herman/Shocker in this series, this version of Clayton has nothing in common with his comic book counterpart outside of his name, his general appearance, and his invention. (Since he did create his own sound powers same as his comic counterpart, he's probably more similar than other examples of this Trope in this series, but he's still portrayed a lot more emotionally well-off here than in his comic debut.)
Sandman (Flint Marko)
- Voiced by: Travis Willingham
- Adaptational Heroism: This version of Sandman is the same as his film counterpart, being a loving father for his daughter. He's also willing to use his powers for good from the start instead of using them for committing crimes in order to make ends meet.
- Strangely, in "Web of Venom" his simulated counterpart acts as one of the villains, despite being Spier-Man's ally and the reason was never brought up.
- Adaptational Wimp: The Flint Marko in this cartoon is not nearly as powerful as his comics counterpart and other versions.
- Always Someone Better: By the time he goes to rescue his daughter, she's already gotten greater control of her powers than he has, and utterly trounces him, albeit in part because he's unwilling to fight her.
- Death by Adaptation: Seemingly the case in his debut episode.
- Decomposite Character: He appears to be primarily based on the more heroic takes of the character, with his much more criminal traits transplanted to his daughter.
- Dishing Out Dirt: As per the course for Sandman, he can turn his body into sand and rock.
- Never Found the Body: He's seemingly killed in his debut episode by Keemia, but Spider-Man doubts that he's dead.
- No-Holds-Barred Beatdown: Suffers this from his daughter when he's unwilling to fight back.
- We Hardly Knew Ye: Gets seemingly killed by his daughter in his debut episode, which is the only time he appears.
- Voiced by: Alex Désert
- Adaptational Nice Guy: Much like his comic counterpart, he is generally a nice guy to people. However, in the comics Jefferson detested mutants and other superhumans—though given it was the Ultimate Marvel universe, this was understandable given the Adaptational Jerkass or even Adaptational Villainy most every hero not named Peter Parker went through and he did warm to Miles's alter ego some time after learning it was Miles. Here, he's actually okay with Spidey and his son's alter ego from the start.
A mutated boy who lives out in the woods.
- Canon Foreigner: He doesn't have any counterpart in the comics.
- Exorcist Head: He turns his head 360 degrees like what normal owls do. Even Spider-Man is freaked out by this.
- Expy: Though likely unintentional, his origin as a boy/teenager who was mutated into a bird-like form by a mad scientist harks very much to that of Joaquin Torres, the second Falcon introduced in Nick Spencer's Captain America. Nocturnal is a bit more noticeably transformed than Falcon is, though, and doesn't have nearly the same network of support looking after him.
- Given his name, he may also act as a Distaff Counterpart to Angela Cairn/Nocturne, a police officer who was also mutated into a being with a bat-like form by a mad scientist, in this case Baron Zemo.
- HeelFace Turn: Somewhat; while he's likely still scrounging other people's food in the woods and generally being reclusive, he doesn't have any ill will toward Spider-Man.
- Tragic Monster: He was transformed from a lab accident that forced him to live out in the woods.
- Voiced by: Sumalee Montano
- Adaptational Job Change: In the comics, she was merely a police captain. Here, she's the chief of the NYPD.
- Commissioner Gordon: Becomes this to Spider-Man.
- Da Chief: This case, literally, as she's the chief of the NYPD.
- Jerk with a Heart of Gold: She's stern and no nonsense, but still deeply committed to helping others out.
- Reasonable Authority Figure: In Season 2, she's one of the few people who earnestly believes Spider-Man is actually good for New York (and is clearly hesitant to help aid Jameson's witch hunt), and even tells him to call her if there's any significant information he's found in regards to the mastermind who wants to hunt Spider-Man down. While she does briefly come into conflict with Spider-Man due to some incriminating circumstances, to the point of having to take him to prison, after Octavius proves he's still around, she's very quick to cooperate with Spider-Man afterward. In fact, she shows a Smile of Approval when Superior Spider-Man disposes of the bomb that was set to destroy the prison.
- Voiced by: Kevin Shinick (Bruce Banner), Fred Tatasciore (Hulk)
A scientist mutated by gamma radiation to transform into a monster when he gets angry.
A member of the Avengers whom Absorbing Man encounters at one point, but is never seen.
- The Ghost: Hawkeye is mentioned by Absorbing Man after his encounter and has yet to make an appearance.
- Voiced by: Roger Craig Smith
One of the founders of the Avengers who is referenced by Peter, but isn't seen until Season 2.
- The Ghost: He is mentioned by Peter in "The Rise of Doc Ock, Part 4" when he is using a trash can lid as a shield.
The Prince of Asgard and a member of the Avengers whom Peter mentions, but doesn't appear.
A member of the Avengers who was captured by A.I.M., along with Hulk and Captain America.
- The Voiceless: She doesn't speak in her appearance.
A member of the Avengers who is after a Vibranium key.
- Animal Motif: Her outfit and codename are modeled after a Black Widow Spider and she gains spider powers which completes the motif.
- Boyish Short Hair: Unlike most versions of Black Widow who has long shoulder-length hair (though she did have short hair in the 80s), this one has a more punk style with the sides shaved short and longer hair on the top. Possibly Justified to be more practical for combat.
- Empowered Badass Normal: Black Widow is already a Badass Normal who's easily able to defeat the already empowered Hydra super soldiers. Once she gains Spider-Powers, she kicks twice the amount of ass and delivers a Curb-Stomp Battle to a Hydra commander combined with her natural talents.
- Fiery Redhead: She's aggressive, feisty and battle driven, but she's still a seasoned and reasonable member of the Avengers.
- Instant Expert: Within a minute of gaining Spider-powers, she's able to get a quickly get a handle of them to the point of easily mopping the floor with a gang of Hydra Mooks.
- Jerk with a Heart of Gold: Stern and no nonsense that she is, no one can deny that she will not hesitate to help others.
- Vague Age: While presumably an adult, it's never brought up how old she is and her character design makes it hard to distinguish her from the other main characters, who are teenagers.
- Voiced by: Mick Wingert
The CEO of Stark Enterprises and one of the Avengers. Spider-Man first meets him during the Stark Expo, where they end up clashing due to Stark mistaking him for an intruder. Said intruder later turns out to be supervillain Ghost, who they defeat by teaming up. They later keep contact with each other, serving as Spider-Man's first link to the Avengers.
- Accidental Misnaming: At the end of "Stark Expo", he gives Peter his autograph, but makes it out to "Paul".
- Let's You and Him Fight: He initially attacks Spider-Man upon meeting him, mistaking him for the intruder at Stark Expo (it actually was Ghost).
- The Mentor: He implies at the end of the episode that he would like to be this for Spider-Man the same way Max Modell is to Peter Parker.
- Secret Keeper: While not outright stated, it's strongly implied by the end of the episode that he has deduced Peter's identity based on details connecting the two (in particular their size and the fact Peter has a hand injury he inflicted on Spider-Man earlier).
- If he didn't figure it out then, then he certainly is in the know after he, Natasha, and Kamala detain Superior Spider-Man. He outright says Peter's name while talking to the other two Avengers.
- Voiced by: Kathreen Khavari
A member of the Avengers sent by Iron Man to investigate a boarding school after its students start displaying out-of-control super powers, but is forced to team up with Spider-Man.
- Adaptational Personality Change: Kamala is more focused on the task and doesn't fangirl over meeting Spider-Man like she does in the comics.
- Justified Trope: In the comics, Peter is an experienced hero and Kamala's a rookie. In this series, Kamala is the more experienced one.
- Alliterative Name: Kamala Khan.
- Always Someone Better: Kamala is constantly being compared to Thor, Captain America, and the rest of her Avengers teammates and she implies her lack of choosing decisions, hence her desire to prove her competence to Iron Man.
- Dare to Be Badass: Shes really excited about participating in her first solo mission undercover to stop, A.I.M. hence her initial intolerance to Spider-Man intruding.
- Easily Forgiven: After the whole Superior Spider-Man incident, she's angry with Peter over his supposed attitude. When Peter confesses what happens, she considers it water under the bridge. "Hello?! I'm an Avenger! We've seen way weirder stuff than that."
- Experienced Protagonist: In contrast to Peter, shes been working with the Avengers for a period of time, and has more recognition as a hero than Spider-Man.
- Fake Relationship: Non-kissing example, Kamala takes off her identity and convinces Peter to do so, tricking the guards by hugging him to pass off the two being a couple.
- Fire-Forged Friends: Through the course of foiling Scientist Supreme and AIM with Peters help, shes slowly grown to accept him as an ally and a friend, even attempting to improve his reputation with the press.
- Rubber Man: The same as her other incarnation.
- Secret Keeper: She is one of the few characters who know Peter's secret identity as Spider-Man.
- Seen It All: When Spider-Man explains "his" behavior as Superior Spider-Man as it actually being Otto Octavius in his body, she has no problem believing it because, as an Avenger, she's "seen way weirder stuff than that."
- Ship Tease: With Peter; while she made them look like a couple to avoid suspicion while on a mission, one can't help how cute they look together.
- Sizeshifter: As per her canon comics counterpart, she can adjust the shape and size of her body parts, mostly preferring to adjust her arms for stronger striking power or legs for improved mobility.
- Voiced by: Aubrey Joseph (Cloak) and Olivia Holt (Dagger)
A duo who come into conflict with Superior Spider-Man.
A member of the Avengers who is mentioned by Max Modell, but doesn't make a physical appearance.
- The Ghost: Vision is mentioned among his fellow Avengers by Max Modell in the season 3 premiere, but doesn't appear.
- Voiced by: Connor Andrade
A member of the Guardians of the Galaxy who comes to Earth to warn Spider-Man about the upcoming Symbiote invasion.
- Voiced by: Sofia Wylie
An intern for the Avengers who helps Spider-Man against the Symbiote invasion.
Totally Awesome Hulk (Amadeus Cho)
- Voiced by: Ki Hong Lee
A teenager with the same powers as the Hulk.
- Hulking Out: He has the same gamma powers as the Hulk, but looks more like his human form.
- Voiced by: Liam O'Brien
The Sorcerer Supreme who aids Spider-Man against the Symbiote invasion and Baron Mordo.