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- In Airplane!, during Ted Stryker's flashback about how he first met Elaine, before dancing with her, he throws his jacket off and strikes the pose, only for someone to throw the jacket back at him.
- Tony P. does the move in Mystery Men, while dancing the Hustle in the Disco room. A couple of the other Disco Boys do it while fighting the Mystery Men; it seems to be not just a dance for them but also a part of their martial arts style.
- Bigfoot is seen doing it in the background on A Goofy Movie, listening to "Staying Alive" on a Walkman, no less.
- In the sequel, everyone does it during the disco scene.
- Chicken Run is supposed to take place in The '50s, but that doesn't stop the rats Nick and Fetcher from striking the pose during "Flip, Flop, and Fly."
- Willard (Chris Penn) strikes this pose during the final prom scene in Footloose.
Live Action TV
- In a game of "Superheroes" on the American edition of Whose Line Is It Anyway, Colin Mochrie does this while playing "Disco Boy."
- On Married... with Children, Al's Let's Get Dangerous! Catch Phrase was "Let's rock." When the show moved to Saturday nights for its final season, Al was in commercials saying "let's disco" and doing this.
- John Travolta did this himself in an opening monologue on Saturday Night Live, referencing his various roles while saying he's not going to do that including Welcome Back, Kotter, Urban Cowboy, Look Who's Talking; and of course Saturday Night Fever.
Travolta: It's just fun to have a new film like Pulp Fiction and forget about the old ones. What is that? Is that a light? points upward in this fashion
- The Jason Alexander episode of Muppets Tonight included a clip of him with Miss Piggy on Muppet Bandstand. At one point he throws her into the air and has time to do an elaborate solo routine before she comes down, including the Staying Alive pose and drenching himself with water in a Flashdance parody.
- Kelso, Fez, and Eric do this at various times in That '70s Show.
- MAD: Alfred E. Neuman strikes the pose on the cover of an issue spoofing Saturday Night Fever.
- Newspaper comics: There's a Garfield strip where Jon does this at a party. The disco ball then falls on his head.
Jon: *strikes pose* BOOGIE! BOOGIE! BOOGIE! *ball falls on head*Garfield: Let's boogie on home. I'll lead.
- In one of his Star Trek memoirs, William Shatner included a picture of himself as Captain Kirk inadvertently striking this pose, with a snarky caption.
- Disco Inferno, of course.
- The dancing zombie in Plants vs. Zombies does this move, which summons four other dancing zombies to join him.
- The cats and dogs representing the player's lives in the Jimmy T. and Jimmy P. stages in Wario Ware: Smooth Moves.
- The way in which Iku Nagae of Scarlet Weather Rhapsody brandishes her spell cards looks a lot like the pose, leading to a lot of doujinshi associating her with disco.
- Junpei Iori of Persona 3 fame performs this exact pose when he finishes the last shadow in an enemy party.
- The "I Will Survive" dancer from Just Dance 2014 does a version of the pose.
- The Simpsons: Disco Stu, as his name implies, does this often, even during a Civil War reenactment.
- Futurama: In the episode "Less Than Hero", Fry adopts this pose when he becomes the retro-themed superhero Captain Yesterday.
- The California Raisins: Stand up everybody, it's the Disco Polka Man!
- The Backyardigans do a version of the pose during "Quest for the Flying Rock".