Kagami: What's with the (bleep) D-like driving!? *
The "bleep" was intentional. It's part of the dialogue.
Or how about in the same episode when, after Tsukasa puts a seashell to her ear and imagines she's floating in the ocean, a crab pops out, prompting a very reasonable scream and making her run to her sister?
Patty describing "Otome Road" and Yutaka's Imagine Spot.
Another favorite is in the "Wawawawasuremono..." ending, which includes Shiraishi singing next to a random couple (who seem to be trying their best to ignore him), and a subtitle pops up pointing at them which reads: "Random couple who readily agreed to be in this video."
The New Years episode was memorable because out of the blue, Tsukasa says some odd dialogue ("Rice cake goes wee!" and "Cheesecake?").
After Konata's famous "a flat chest is a status symbol" scene, Tsukasa replies "I guess I'm safe too".
In episode 21, where they go to the Daibutsu in Nara, and Konata calls the figures around the main statue "the options".
Konata: Hmm... if this dude's the main one... then the little ones around him... are all the Options!
A blink-and-you'll-miss-it moment in the MMORPG part of the OVA: When Konata and co. do a team victory pose, a random male character joins in on the far right, and then just walks away without anyone noticing.