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- In One Piece, Emporio Ivankov ate the Horm-Horm Fruit which allows him to inject hormones from his fingertips, among its many applications include male/female hormones which can turn himself or any recipient into an opposite sex.
- In a different way, Mr. 2 Bon Clay ate the Clone-Clone Fruit which lets him alter his body into someone else's that he had touched once, including women.
- The Skrulls in the Marvel Universe can change their sex as well as appearance. In Runaways Super Skrull in training, Xavin, is engaged to Karolina for an Arranged Marriage set up by their parents as a peace keep measure between their races. Karolina isn't interested not because she isn't sold on the marriage thing, but because she's a lesbian. Xavin handily solves this by showing her his/her female form and and Karolina has no complaints.
- One of the many magic keys from Locke & Key allows a person to do this.
- In Jurassic Park the cloned dinosaurs were able to get around the genetic manipulations that made them all female thanks to the frog DNA used to complete their genomes.
- In Zerophilia, a person with an extra Z chromosome will change gender after an orgasm. By having sex with another zerophiliac, he/she will be Mode Locked into the opposite gender than what he/she was before then. The movie follows a boy named Luke, who has this condition and goes by the name Luca as a girl, pretending to be his own cousin. He/she eventually gets tricked into being stuck as Luca. Fortunately, his girlfriend Michelle and her brother Max are actually the same person, so their relationship works out.
- Virginia Woolf's Orlando more or less spontaneously turned into a woman. The League of Extraordinary Gentlemen's reinterpretation has that happen every few years, owing to his/her father Tiresias.
- In C. J. Cherryh's Chanur Novels stsho are somewhat prone to "phasing" with little warning, assuming a new gender and personality.
- M.C.A. Hogarth's Jokka can change genders at two points in their life, once in the first seven years of their life and again in the second seven. Some never Turn, others Turn once, some Turn then Turn back to their original gender, and a few rare individuals have the chance to be all three sexes.
- In F.M. Busby's The Breeds of Man geneticists searching for a cure to an infertility plague caused by an AIDS cure Gone Horribly Wrong accidentally turn some people into serial hermaphrodites that alternate between male and female monthly based on menstruation.
- The Left Hand of Darkness has a planet where the human-descended inhabitants are asexual most of the time, except when they're in "kemmer" and become either men or women, as well as very lustful.
- One of Neil Gaiman's short stories in the Smoke and Mirrors anthology concerns the massive paradigm shift that society undergoes when an experimental gene therapy intended to treat cancer has the unexpected side effect of performing an overnight and totally reversible Gender Bender.
- The Monster of the Week in The X-Files episode aptly titled "Gender Bender" is a member of an (apparently) alien humanoid species whose biological gender changes every time after they have sex.
- Mork from Mork & Mindy states that Orkans' "parts are interchangeable"
- The 21st-century version of Doctor Who has explicitly confirmed that Time Lords sometimes change sex on regeneration, although fans had speculated about the possibility for decades and it had never been explicitly ruled out.
- A notorious convention anecdote in relation to Babylon 5 has J. Michael Straczynski responding to all the Ho Yay fan reaction to Londo and G'Kar by distributing a joke script to the actors in which G'Kar changed sex to become female, revealing that Narn are biologically capable of doing it as a response to being in an unduly one-gender environment, and promptly tried to seduce Londo, while still being played by Andreas Katsulis. The actors were apparently entirely enthusiastic about it.
- In GURPS "hermaphromorph" is a five-point trait that allows a character to do this.
- Eclipse Phase has a "sex switch" BioMod that enables one to do this (and comes standard with Pleasure Pods), though the process takes a week.
- In Munchkin, the 'Freudian Slipper' allows the user to change sex (in-game, obviously) whenever it suits them most. If they lose the slipper and they're not their (real-life) biological sex, however, the former user is at a disadvantage in their next combat as normal for Sex Change Curses.
- In Kitsune: Of Foxes and Fools Hayaku can change genders at will, for free at the beginning of each turn and by spending one foxfire any other phase. Saski and Sareiko had a competition to see who could seduce them first, Saski impregnated Hayaku with Ai & Midori while Hayako knocked up Sareiko with Seiki.
- Changelings in Eberron are born asexual, develop their proper gender in early puberty, and then become capable of fully shifting sexes when their powers fully mature in mid-puberty.
- One of the tricks available for Lunars in Exalted. Their patron god-goddess-whatever-it-feels-like-today Luna does so on a regular basis, including appearing as a pregnant man on occasion.
- Incubi and succubi have shapeshifting powers in Amber Panyko Williams' Dan and Mab's Furry Adventures universe. This includes gender changing at will, though it takes much time and practice to do well. Abel Rewanz demonstrates the boy-to-girl morphing in strip #690, and again in strip #1038, much to Daniel's chagrin.
- The artificial lifeform Penelope from Ben Nu~nez's Just Another Web Comic was built as a sentient sex toy by Mad Scientist Eryl Keydon. Penelope can change gender and species on command, though she seems most comfortable as a female feline. See volume 2, page 32 where Penelope recites her tech specs, though be advised: this web comic is patently Unsafe For Work.
- Alchemy the reaper from Dangerously Chloe is an Anthropomorphic Personification of Death and doesn't have a gender as such, instead appearing as "Al" (male), "Kimmy" (female), or as a classic skull-faced Grim Reaper depending upon what the soon-to-die person expects to see. Otherwise their gender depends upon how masculine or feminine they're feeling.
- In the Chakona Space universe skunktaurs, a sister project to the chakats, can switch between male and female at will. Though they also have an annual cycle that forces a sex change.
- In Metamor City people with the androgyne variation of the curse can change sex at will, though they need to spend more time as the opposite gender that they were born with. Considering that the original version of the curse was a one-way Gender Bender.
- Whateley Universe has a few, naturally enough, with Heyoka, Reach, Jimmy T., and Chimera being the main examples. However, each of these cases has some degree of Blessed with Suck to them: Heyoka changes uncontrollably depending on the spirits which s/he bonds with, Jimmy T. has trouble controlling who (or what) s/he shifts into while asleep, Chimera is actually two Half-Identical Twins sharing a single body, and Reach shifts on a semi-random basis every two weeks or so.