The landlord that hates tenants, and he especially hates the hero, whom he considers a no-good scum of the earth.
Similarly to Nosy Neighbor, this character is always on the lookout to catch the hero doing something against the house rules.
Pretty much anything below being a model tenant will earn the landlord's scorn and an infuriated visit to the offending tenant's apartment: making a noise, coming home too late, bringing another person home, etc.
A common trope is that the tenant is late with the house-rent, and has to argue at length with the landlord to be allowed a few days' respite.
A subtrope of Landlord. Compare the Cranky Neighbor.
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Anime & Manga
Sumi's former landlady in Stepping On Roses qualifies, though not without justification: The gang of adopted children Sumi's brother brings home with him are extremely noisy, and Sumi and her brother are well behind on their rent payments.
Surprisingly for this trope, the protagonist's landlord in Sekirei actually kicks him out after catching him with a girl in his apartment. Surprising in that it sticks: He's evicted for good and has to find a new home while taking care of his new Magical Girlfriend.
Dr. Chaos's tenant in Ghost Sweeper Mikami, due to him never really paying his rent. She does have some Pet the Dog moments in the "magic sweeping brooms" episode.
Peter Parker's second apartment, the one for much of the 1970s and 1980s, had a nasty landlady named Mamie Muggins. She was cranky, judgemental, and quick to issue threats. Though sometimes she showed a softer side, helping to furnish an apartment or being a protective aunt to her favorite niece.
Averted in, of all places, Sin City. Dwight and Wallace's landlady was actually pretty sweet and wanted both men to get a nice girlfriend.
Mr. Shickadance (quoted above) from the Ace Ventura: Pet Detective movie and video game, is a cranky old man who hates animals. Despite his warnings for Ace not to bring any work home, Ace secretly keeps more than a dozen housepets in his room, having trained the animals to hide when the landlord appears.
Mrs. Peenman from The Mask (pictured above). Stanley lives in fear of her, while he also takes revenge whenever he changes into his alter ego, the Mask.
The (in)famous Funny or Die video "The Landlord" casts Will Ferrell's daughter, barely past infancy at the time, as one of these to comedic effect.
The Mexican comedy El Chavo del ocho had Señor Barriga ("Mister Belly"), the fat owner of the neighborhood the characters lived in, who would occasionally show up to collect the rent. Despite his gruff appearance, Barriga was actually a nice man—it's just that characters like Chavo and Don Ramon unintentionally provoked his anger almost all the time.
Time-travelers Picard and company take up residence in a boarding house in 1890s San Francisco in the "Time's End" two-parter. When their landlady irately demands the long-overdue rent, Picard distracts her by offering her a role in his "acting troupe's" upcoming play.
In an episode of My Name Is Earl, Earl had one of these back in the day. He found a walkie-talkie that she was able to hear through her hearing aid, and convinced her that he was God, taking advantage of her devout religiosity. As "God", Earl convinced her to be nicer to him, his then-wife, his brother, and their friends. Eventually she became a nun, and Earl had to tell her what he did because it was on the list, thus giving her a Crisis of Faith.
Horatio Hornblower: In "Loyalty", Mrs Mason is Hornblower's Cranky Landlady. She's nervous when he owns her rent as officers starve on half-pay and is displeased that he goes to to play cards. Even though he actually usually earns some money because he's a mathematical genius and skilled whist player. When he brings his friend Mr Bush, she grunts whether he has money to pay her. Her daughter and Christmas Cake Maria is crazy about him, which Mrs Mason doesn't like either, because her husband was a sailor and died at service, and she might be angry at Hornblower because he's not interested in Maria romantically and considers her just a friend.
The Doctor Who episode "Night Terrors" features a landlord as scary as anything else the Doctor sees that night.
In Always Sometimes Monsters, your landlord's sick of waiting for you to get your act together and pay your rent. You owe him $500, and if you don't get it together, you're outta there!
Eulice Deville of Ménage à 3. When Gary introduces his new roommates, she ignores their pleasantries and shouts, "JOBS!?" She can detect a deadbeat tenant a mile away. She once tracked Gary across the city and found him hiding in a restaurant broom closet. She reacts to Mr. Dithers firing Dagwood yet again with an Evil Laugh.
In Dustpit Follies, the landlord will hound the main characters till they pay the rent, even if that means breaking a hole in the roof to drop in.
Mr. Bean: The Animated Series had Ms. Wicket. (Though she did not appear in the live-action series, she was mentioned in the diary.)
In The Simpsons episode "Behind the Laughter", Homer had a stint on Broadway as the landlord in Rent II:
Where is the rent?
I must have the rent!
Dollars, dimes and nickels,
I need them all right now!
Bullock's landlord in Batman: The Animated Serieshated him, for reasons that were arguably justified, mainly his abrasive nature and the fact that his slovenly habits caused a major vermin problem in the building. The man ended up sending Bullock death threats in an effort to get him to move, coincidentally at the same time that a mobster that Bullock had put away years before was released on parole and tried to get revenge.
Pete is the Cranky Landlord at the Houseof Mouse. For some reason he want Mickey and the gang out of the club and he uses different plans to do it. Of course, he always fails.
Clint Clobber was created by Gene Deitch for the Terrytoons studio in the late 1950s. He was superintendent and janitor for the Flamboyant Arms apartments, where anything that could go wrong for him did.