J. Jonah Jameson has high blood pressure and medication to take, whilst Miss Brant has a charge from his wife to make sure he doesn't lose his temper, a long history of being treated like his dogsbody and an intercom with a loud buzzer. Revenge is had.
A buzzer so loud, that when the desk gets rattled every time, it's hard to tell if it was because of the vibrations or Jameson's startled jumps.
While probably unintentional, MJ's line to Peter accusing him of a possible relationship between him and Gwen Stacy. "How come you never mentioned her? She's your lab partner, you saved her life, she thinks you're a genius and she had her polished fingernails all over you?" Just the way she finishes with the "polished fingernails" bit makes it sound like Arson, Murder, and Jaywalking, as if Gwen having polished fingernails is part of what's wrong. Then again, MJ issort of the tomboy to Gwen's girly girl.
The scene where Peter Parker, under the possession of the symbiote, conclusively disproves both that Evil Is Cool and Evil Is Sexy by strutting down the street like someone out of Saturday Night Fever with a spectacularly daft Emo haircut, convinced he's the hot-stuff despite the looks of confusion, bemusement and disgust from every woman he crosses paths with, is very good for a chuckle.
I distinctly remember a couple of women looking intrigued. One was even staring at his ass. When I realized this, I could not stop laughing for 3 minutes straight.
During the final battle, Peter and Harry are flying downward on his glider trying to catch a falling Mary Jane. Peter asks if it can go faster, Harry responds with 'Hang on!'. Peter aptly notes 'To what!?'
Also when Harry says 'Hey Pete! Am I interrupting!?' when intruding on a Peter/MJ moment.
While watching the final battle, JJJ gets conned by a kid. See it here.