Adrenaline Makeover - played straight and subverted; June Havens certainly Took a Level in Badass after significant bits of the plot, but up until the halfway point of the movie she got progressively more worn down as the action progressed.
Always Save the Girl - subverted: June deliberately gets herself kidnapped so that she can get back together with Roy, assuming that he'll save her. He does, but he has no idea that she's there at the time, as he's around for a completely different reason. He does take a moment in the middle of a firefight to step out from cover and kiss her when she guilt talks him into it, though.
Angrish - June's reactions in much of the first half of the movie.
Anti-Hero - Roy Miller may have killed dozens of innocents in cold blood and stolen the perpetual energy battery; he definitely kills dozens of agents in cold(ish) blood and drags an unknown innocent into the fight with him, though it's fairly obvious that he has little to no choice about the matter.
"Sorry, reflex. Hit me again; I won't dodge this time."
Artistic License - Physics: Subverted, but not at first. The Zephyr is presented as a true perpetual motion machine - it will never run out of energy, ever. However, over the course of the film, the battery slowly builds up heat and eventually explodes, taking out the villain in his moment of triumph. Despite Roy's reassurance to Simon that "[he's] a smart kid - [he'll] figure it out," he clearly won't: The audience knows this, but Roy doesn't, meaning he still fails physics.
Badass Driver - both Roy and June. June manages to pull off some amazing moves going the wrong way on the highway while controlling a runaway car from the back seat reaching over the corpse of the former driver - Roy calls her a "natural."
Belligerent Sexual Tension: Lampshaded by June after she's had a truth serum injected into her body and admits to Roy that all their crazy adventures make her want to have sex with him. His reaction is priceless.
Bodybag Trick - done backwards; a supposed corpse is wheeled out of the hospital.
Bond Villain Stupidity - Yep. Mind you, at that point, the bad guy thought he had already won, so the survival of the good guys was reasonably irrelevant.
Chekhov's Gag - almost everything Roy says to June, funny or not.
Chekhov's Skill - At one point Roy demonstrates to June how to escape from a reverse choke hold. A few scenes later a would-be assassin puts her in - what else - a reverse choke hold.
Cloud Cuckoo Lander - Every screw in Roy's lovable head is loose, as exemplified by this line:
Roy: No one follows us, or I kill myself and then her!
Completely Missing the Point - Rodney, for a man going for lieutenant, you're more dense than a concrete training building.
Dueling Movies - Came out around the same time as Killers and while their plots are not identical, they are similar enough that the general public noticed the oddity of their close release dates.
Heroic Sacrifice - Roy, taking a bullet for Simon. He survives, though.
Imperial Stormtrooper Marksmanship Academy: done so much it stops being funny. The baddies are entirely worthless with guns, and can't track Tom's character while he calmly walks over to kiss the girl - let alone while he's running. They also have trouble hitting still targets.
Lodged Blade Recycling: June accidentally impales an assassin with a knife, followed by the assassin pulling it out of himself to attack June with.
Made of Iron - The assassin on the train. Wow. Most of the stuff is just standard movie things, until he gets stabbed in the chest with a knife! The assassin also No Sells June whacking him in the head with a frying pan.
Neutral Female - June, but not during the action sequences; she's actually this during much of the plot, being very decidedly on one side or the other when action breaks out.
Nice Guy - Roy, even when thrown into walls by super-strong Assassins, is a soft-spoken gentleman who does not curse or use swear words once throughout the entire movie. In one of the heart-warmeyer moments of the film, Roy has left June a note saying he was happy to have met her, and a second, reminding her to 'Eat a good breakfast, June'. He'd left her an omelette.
Precision F-Strike - June gets one in an argument with Roy about his "warning" not to get on a plane not being very effective, she then says that a better warning would've been saying something like "June, if you get on this plane, you will fucking die!"
Sole Survivor - Simon, Roy, or Fitz, depending on your point of view. The massacre happens in the backstory, and most of the movie is trying to figure out exactly who did what to whom.