Characters: The Powerpuff Girls
- The Heroes- Blossom, Bubbles, and Buttercup
Residents of Townsville
- Residents of Townsville- Professor Utonium, The Mayor of Townsvile, Sara Bellum, the Talking Dog, the Narrator, Ms. Keane, Mitch Mitchelson, Elmer Sglue, and Stan and Sandra Practice.
- Main Antagonists- Mojo Jojo, Him, Fuzzy Lumpkins, the Gangreen Gang, Princess Morbucks, Sedusa, the Amoeba Boys, and the Rowdyruff Boys.
- Minor Antagonists- The Gnome, Dick Hardly, Femme Fatale, Mr. Mime, Boogie Man, The Smiths, Major Man, Abracadaver, Mike Brikowski, Max Von Nitrate, Lenny Baxter, Mask Scara, The Alien, Giant Monsters, Mojo's Monkey Army, Roach Coach, The Broccoloids, The Sandman, The Robbing Leach, The Powerbluffs and the Burglar