Dudley-Do-RightThe titular character, and an officer in the Royal Canadian Mounted Police, opting to marry Nell, and capture Snidely Whiplash.
Nell FenwickThe beautiful daugher of Inspector Fenwick, and the target of the affections of Snidely Whiplash and Dudley-Do-Right.
Snidely WhiplashThe main antagonist of the episdes, a very archetypical villain, acting evil generelly because he is evil, and wanting to marry Nell.
Inspector FenwickThe inspector of Dudleys police force, and the father of Nell.
HorseThe horse of Dudley-Do-Right, and the one who always saves Nell, though Do-Right takes credit.
HomerThe only named henchman of Snidely Whiplash, intorduced when he makes a fake band for smuggling fur.
Faithful DogThe faithful dog of Dudley-Do-Right, in reality a timber wolf used by Snidely Whiplash.