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  • Adventure Time:
    • Season 6 has Orgalorg, an Eldritch Abomination who seeks to absorb the powers of the Catalyst Comet for his own gain.
    • Season 10 has Gumbald, Princess Bubblegum's Evil Uncle.
  • Amphibia:
  • Avatar: The Last Airbender:
  • Batman Beyond: Derek Powers / Blight for the first season. His takeover of Wayne-Powers drives the season's plot and ends with Blight's supposed death in the finale. Although mentioned, Powers never returns in the series afterward.
  • Ben 10:
    • Ben 10 (2005):
      • Season 1: Vilgax, who indirectly makes several attempts to take the Omnitrix from Ben while he's still recuperating from the injuries he received from Xylene's ship, only going after Ben himself once he has fully recovered.
      • Season 2: Kevin 11, who ends up mutating into an amalgam of Ben's first ten alien forms and, blaming Ben for this, attempts to get revenge on him. He joins forces with Vilgax in the season finale to take the Omnitrix from Ben before a failed attempt at a double-cross leaves them both trapped in the Null Void.
      • Season 3: Zs'Skayr, who is revealed to be The Man Behind the Man to the horror-themed aliens Ben and his family come across, planning to block out the Earth's sun.
      • Season 4: The Forever King, Driscoll, a disgraced Plumber who joined the ranks of the Forever Knights and serves as The Man Behind the Man to recurring villain, Enoch.
    • Ben 10: Alien Force:
      • Seasons 1 and 2: The Highbreed, an alien race that seeks to wipe out all life in the universe to ensure they don't die alone after years of inbreeding to maintain their blood purity have left them on the verge of extinction.
      • Season 3: Vilgax, who makes his return after stealing the powers of ten heroes so he can get his revenge on Ben.
    • Ben 10: Ultimate Alien:
      • Season 1: Aggregor, before Kevin sacrifices his sanity to gain the power necessary to stop him, leading him to become the Final Boss, Ultimate Kevin.
      • Season 3: A Big Bad Ensemble between the Forever Knights (now led by the founder, Sir George), Diagon, and Vilgax. Sir George ends being A Lighter Shade of Black, only seeking to end the threat of Diagon to the point he would cooperate with Ben's team. After Diagon kills George, he is successfully usurped and absorbed by Vilgax, making the latter the Final Boss.
    • Ben 10: Omniverse:
      • A New Beginning: Khyber
      • Malware's Revenge: Malware
      • Incursean Invasion: Milleous
      • Duel of the Duplicates: Albedo
      • Galactic Monsters: Zs'skayr and Lord Transyl
      • The Evil Rooters: Proctor Servantis and Rooters
      • The Mad Nightmarte: Mad Ben
      • The Time War: Maltruant
    • Ben 10 (2016):
      • Season 1: Vilgax for the four-part finale, "Omni-Tricked".
      • Season 2: Vilgax, until the High Override usurps him in the five-part finale, "Inner-Vasion".
      • Season 3: The Forever Knight and Kevin 11, until the latter is Demoted to Dragon for the two-part finale, "Roundabout".
  • Beware the Batman: Anarky serves as Batman's Arch-Enemy but he tends to play a secondary role to the bigger villains: Ra's al Ghul in the first arc, then Deathstroke in the second arc.
  • Big Hero 6: The Series:
    • Season 1: Obake.
    • Season 2: Diane "Di"/Liv Amara for the first half, Chief Cruz for the second until Trina usurps the role for the two-part finale.
    • Season 3: Noodle Burger Boy.
  • The Boss Baby: Back in Business
    • Season 1: Bootsy Calico
    • Season 2: Frederic Estes
    • Season 3: Happy Sedengry
    • Season 4: Maria Maria
  • DuckTales (2017):
    • Season 1: Magica De Spell
    • Season 2: General Lunaris
    • Season 3: Bradford Buzzard and F.O.W.L.
  • Each season of Fast & Furious: Spy Racers is a mostly self-contained story that takes place in a different locale and has a different main villain.
    • Season 1: Shashi Dhar
    • Season 2: Rafaela Moreno
    • Season 3: Cleve Kelso
    • Season 4: Moray
    • Season 5: Sudarikov
    • Season 6: Dann
  • Though Xanatos and Demona were the overarching villains of Gargoyles, each of the major multi-part storylines had their own villains (with the aforementioned two sometimes — but not always — in the position):
    • "Awakening": Xanatos and Demona as a Big Bad Duumvirate
    • "City of Stone": Demona
    • "Avalon": The Archmage
    • "The Gathering": Lord Oberon
    • "Hunter's Moon": Demona and the Hunters, in opposition to each other.
    • The non-canonical Goliath Chronicles had Castaway and the Quarrymen.
  • Gravity Falls
  • Green Lantern: The Animated Series: First Atrocitus, then the Anti-Monitor, and finally Aya.
  • Jackie Chan Adventures:
    • Season 1: Shendu (though he causes trouble in every following season afterwards)
    • Season 2: The Demon Sorcerers
    • Season 3: Daolon Wong (until he's usurped by Shendu in the finale)
    • Season 4: Tarakudo
    • Season 5: Drago
  • Jurassic World: Camp Cretaceous
  • Kaeloo: Season 5 focuses the main characters fighting Game Rule, who gets a Heel–Face Turn and befriends them at the end of the season.
  • Legends of Chima:
    • Team of Cragger and Crooler while Crooler is manipulating Cragger with the Persuader Plants
    • Worriz after Crooler breaks the Crocs' pact with the Wolves, with Crooler trying to regain lost footing
    • Cragger after he's corrupted by the Fog of Destiny and has Crooler imprisoned
    • Bat, Scorpion and Spider tribes led by Scorm and Spinlyn in season 2
    • Sir Fangar and the Saber-Toothed Cat, Vulture, and Mammoth tribes in season 3
  • The Legend of Vox Machina
    • The two-parter pilot episode has Brimscythe, a blue dragon terrorizing the lands of Tal'dorei.
    • The rest of season one has Lord and Lady Briarwood, the current rulers of Whitestone after staging a coup that killed most of Percy's family.
    • Season two has the black dragon Umbrasyl, member of the Chroma Conclave, who was ordered by Thordak to hunt Vox Machina down.
  • The Lion Guard:
    • Season 1: Janja
    • Season 2: Scar
    • Season 3: Makucha
  • My Little Pony: Friendship Is Magic uses a different villain for most of its multi-part episodes:
  • Ninjago
  • For the first time, South Park features its first season-long villain in season 19. It's revealed that Leslie is behind a plot to destroy the town of South Park. She's actually an advertisement that became sentient, and is planning to use PC people to get the town to gentrify and make South Park too expensive for people to live in anymore.
  • The Spectacular Spider-Man was divided into 3-4 episode arcs. Though Tombstone and Norman Osborn vied for the position of Big Bad of the overall series, each arc had its own central villain(s):
    • Arc 1: The Lizard
    • Arc 2: Tombstone
    • Arc 3: Green Goblin
    • Arc 4: The symbiote, ultimately leading into Venom
    • Arc 5: The Master Planner, aka Doc Ock
    • Arc 6: Venom
    • Arc 7: Tombstone, Doc Ock, and Silvermane in an Evil Versus Evil scenario, though it turns out they were all being played by the Goblin
    • Arc 8: Green Goblin ultimately revealed to be Norman Osborn
  • Spider-Man: The Animated Series: While the Kingpin is the overall Big Bad, each season (save the fourth/Partners in Danger Arc) has an additional prominent threat:
    • Season 1: Venom
    • Season 2/Neogenic Nightmare Arc: Morbius
    • Season 3/The Sins of The Father Arc: The Green Goblin
    • Season 5: The Red Skull and Electro for the Six Forgotten Warriors arc; Doctor Doom during the Secret Wars three-parter; and Spider-Carnage in the Spider Wars finale.
  • Star vs. the Forces of Evil
  • Since Star Wars: The Clone Wars follows an anthology-format of loosely connected Story Arcs, most of the villains appear in only one story arc and never show up again. Also, an interesting case is that a number of them are small scale lieutenants to Count Dooku, instead of independent antagonists with an agenda of their own, although there are some. Examples of this type include:
    • Malevolence Arc: General Grievous
    • Search for R2-D2 Arc: General Grievous
    • Florrum Arc: Hondo Ohnaka, who captures Dooku and tries to ransom him off to the Republic, only to take Anakin and Obi-Wan hostage for ransom as well when they arrive to verify Hondo's claims that he has Dooku hostage.
    • Blue Shadow Virus Arc: Dr. Nuvo Vindi
    • Ryloth Arc: Wat Tambor
    • Holocron Arc: Cad Bane
    • Geonosis Arc: Poggle the Lesser
    • Saleucami Arc: General Grievous
    • Mandalore Arc: Pre Vizsla
    • Zillo Beast Arc: The Zillo Beast
    • Boba Fett Arc: Boba Fett and Aurra Sing
    • Ziro the Hutt Arc: Cad Bane, acting as The Heavy for the Hutt Council. He breaks Ziro the Hutt out of Republic captivity, and later tries to hunt him down when Ziro escapes Hutt captivity
    • Nightsisters Trilogy: The plot focuses on the Evil Versus Evil scenario between Asajj Ventress and Count Dooku, with the heroes caught in the middle
    • Mortis Arc: The Son
    • Citadel Arc: Osi Sobeck
    • Trandoshan Arc: Garnac
    • Mon Calamari Arc: Riff Tamson
    • Umbara Arc: Pong Krell
    • Zygerria Arc: Queen Miraj Scintel
    • Obi-Wan Undercover Arc: Moralo Eval and Cad Bane, who are hired by Dooku kidnap Palpatine. Moralo Eval is initially in charge, until he is sidelined by Dooku in favor of Cad Bane. After the two are captured, Dooku becomes the Final Boss of the arc.
    • Onderon Arc: Sanjay Rash, with General Kalani as the Dragon-in-Chief
    • Young Jedi Arc: Hondo Ohnaka with General Grievous as the Final Boss
    • Shadow Collective Arc: Darth Maul, with Pre Vizsla as a Big Bad Wannabe
    • Ahsoka the Fugitive Arc: Barriss Offee
    • Order 66 Arc: Nala Se, who is working to prevent the discovery of Order 66. In the arc's final episode, the Arc Hero Fives learns that the conspiracy extends to Chancellor Palpatine himself
    • The Disappeared: Mother Talzin
    • Yoda Arc: Darth Sidious, the series' Big Bad, sends his apprentice Count Dooku to sabotage the Jedi's investigation of Sifo-Dyas, and later, with Dooku's assistance, casts an illusion in an attempt to break Yoda.
    • Bad Batch Arc: Admiral Trench and Wat Tambor
    • Ahsoka's Journey Arc: Mard Krim, with Maul as The Man Behind the Man
    • Siege of Mandalore Arc: Maul for the first half of the arc. After he is captured, Darth Sidious activates Order 66, forcing Ahsoka's clones, led by Commander Rex to turn on her. After Ahsoka frees Rex from his inhibitor chip, Jesse becomes the Post-Final Boss in the final episode of the series
  • Star Wars Rebels:
    • Season 1: The Grand Inquisitor and Grand Moff Tarkin
    • Season 2: Darth Vader
    • Season 3: Grand Admiral Thrawn and Maul
    • Season 4: Thrawn and Palpatine
  • Along with fighting the Monster of the Week, Steven Universe has major villains that gradually reveal the backstory of the Crystal Gems.
  • Tales of Arcadia:
  • Tangled: The Series:
    • Season 1: Varian
    • Season 2: King Edmund
    • Season 3: Zhan Tiri, with Cassandra as The Dragon
  • In Teen Titans (2003):
    • Seasons 1 & 2: Slade.
    • Season 3: Brother Blood.
    • Season 4: Trigon with Slade as The Dragon.
    • Season 5: The Brotherhood of Evil, led by the Brain.
  • In Total Drama, every season except for Pahkitew Island has a character that takes the role of the main antagonist.
  • Wakfu
  • Winx Club:
  • X-Men: The Animated Series:
    • Season 1: Master Mold and the Sentinels
    • Season 2: Mister Sinister
    • Season 3: Dark Phoenix
    • Season 4: Apocalypse
    • Season 5: Magneto


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