- Big-Lipped Alligator Moment: Many per episode. It comes with the territory.
- Crazy Awesome: Ichijou.
- Ear Worm: The opening themes. All of them.
- Ensemble Dark Horse: Despite being called plain, Kurumi sure has a quite the fanbase because of her sexiness.
- Les Yay: Done quite a few times. One including Behoimi and Media moving around behind a curtain with some rather suggestive dialogue while they disarm a bomb. Also in episode 15, where Becky tells Sayaka to do something to keep Himeko from jumping out the back of a bus teetering on the edge of a cliff (long story) and her answer to the problem is...well, this.◊
- Again with Behoimi and Media in the final episode when they swap outfits, and Behoimi admits she's a little turned on when Media shows it off.
- Despite Sayaka teasing with Himeko and Miyako in episodes 15 and 17, respectively, there's actually more subtext between her and Kurumi. They even tease each other every once in a while (Kurumi usually becomes a Deadpan Snarker when it comes to Sayaka's naiveness and Sayaka even teases her, too).
- Himeko towards Rebecca.
- In the English language trailer, Otome Akiyama is called "the tyke" and and Suzune Shiratori "the dyke".
- Moe: Subverted by Kurumi, who actually works at a Moe cafe. Well, it would've been a Moe cafe... if it weren't for Kurumi's lack of "moe factor". Otome, too.
- The cafe itself isn't really Moe Cafe-ish anyway, what with not only the inside but the owner himself being in stark black and white.
- Akira Miyata. And yes, her haircut is cute.
- Played completely straight with Becky of course.
- Nightmare Fuel: Some of Ichijou's antics qualify.
- Viewer Gender Confusion: Mesousa is actually male, but it can be hard to tell thanks to his feminine voice and tendency to crossdress.
- The Woobie: Mesousa, Kurumi, Miyata, and somehow Otome.
- Becky can sometimes also become this, especially when she cries.
- Woolseyism: As if SHAFT's own cultural references weren't enough, ADV Films mixes in some of their own, including a Mel Gibson reference in the island episode.
YMMV / Pani Poni Dash!