- One of the three "Genie Rules" stated by the Genie from Aladdin prohibits him from killing anyone.
- Subverted in Aladdin: The Return of Jafar, Jafar doesn't have any real problem with the rule, since he can still invoke a fate worse than death in either case or cause circumstances that result in a person's death as long as he isn't the one directly pulling it off. A Running Gag in the movie is, after mentioning that Genies can't kill, someone says "You'd be surprised at what you can live through!"
- Manolo from The Book of Life, is firmly opposed to the idea of killing the bulls he fights, even though it earns him the ridicule of his family and the townsfolk, sans Maria.
- Averted in The Incredibles. None of the Incredibles have any problem using deadly force in self-defense, and a lot of mooks die as a result. Bob does stop short of deliberate murder even in the grip of his Unstoppable Rage at believing his family was just killed, however.
- Megamind knew that Metro Man went by this rule, always sending him to jail. It was one of things that made their battles seem like a game to him. Unfortunately, when he created Titan to take Metro Man's place, he didn't anticipate Titan making his own rules.
- In the infamous flashback scene in Batman Beyond: Return of the Joker, the Joker plays a Berserk Organ with what he did to Tim "Robin" Drake. Just seeing the boy made Bruce beyond pissed; hearing the Joker's tale about how it all happened... he really was tempted to "break him in two". The film implies he actually would have done it, if Tim hadn't killed him first. Joker thinks otherwise.Joker: Oh Batman, if you had the guts for that kind of fun you would've done it years ago. I, on the other hand... (proceeds to attack)
- The wisdom of this trope is called into question by a different Robin, Jason Todd in Batman: Under the Red Hood centered around The Joker once again.Batman: You don't understand... I don't think you've ever understood.Jason Todd: What? What, your moral code just won't allow for that? "It's just too hard to cross that line"?
Batman: No! God almighty, no. It'd be too damned easy. All I've ever wanted to do is kill him. Not a day goes by that I don't think about subjecting him to every horrendous torture he's dealt out to others, and then... end him.
The Joker: Aww, so you DO think about me!
Batman: But if I do that - if I allow myself to go down into that place... I'll never come back.
Jason Todd: Why? I'm not talking about killing Penguin, or Scarecrow, or Dent. I'm talking about him. Just him! And doing it because... because he took me away from you.
- Big Hero 6:
- Since Baymax of was programmed to be a healthcare robot, he naturally has an aversion to injuring people, much less killing them. Hiro gets around this by removing Baymax's original Personality Chip so he could be ordered to kill Yokai. When Baymax later comes to his senses and realizes what Hiro did, he physically locks Hiro out from accessing his Personality Chips to prevent Hiro from forcing him to kill again.
- The rest of the titular team doesn't agree with Hiro's actions either, and after he grows out of his Revenge Before Reason mindset, this trope becomes more or less their code.
- The Hunchback of Notre Dame: Quasimodo refuses to kill anyone. There is a moment in the first film were he held the dagger he disarmed from Frollo and threw it and gave him a speech about Frollo being the monster instead of stabbing him. He even saved him when both Esmeralda and Frollo were about to fall.
Thou Shalt Not Kill / Animated Films