With the series being so metafictional and having an author obsessed with tragedy, this should be expected.
- Episode 2 has Anteaterina's frustration over Rue being better than her.
- Episode 3 has Ebine's backstory and her weeping over her lost husband.
- A small one, but the look on Rue's face when she's dancing and falls over because of Duck's distraction, hitting the ground.
- Duck's loss of identity early on in the series, as she grapples over who Tutu is in relation to herself and whether she's just a part in a story or not.
- It's hard not to feel really bad for the lamp in episode 5.
- Mytho is affectionate with Rue at the end of episode 5. Instead of being happy or relieved, though, Rue seems terrified of it as she pushes him away and runs off.
- In episode 6, when Mytho gets the heartshard of fear and becomes terrified of Princess Tutu, Ahiru's breakdown is very upsetting and really marks a turning point in the series. It's not her fault the shards she returns to him are negative emotions, and seeing her pure intentions cause the boy she's trying to make happy whole gallons of pain, it's hard to blame her for sobbing her eyes out. "I just wanted to make him smile!"
- Edel's death in the first season finale. The fact that as she burns to death, her last request is that Mytho and Ahiru dance for her does not make it any happier.
- In season 2, Duck grows increasingly isolated from what's going on, has no idea what's happening or why, and feels like she can't do anything. It takes lectures from Mr. Cat, Fakir, and a Sadistic Choice from Drosselmeyer to pull her out of it.
- Rue's meeting with the Raven in episode 16. Despite terrifying her with threats of violence should she go against his will and emotionally beating her down by calling her ugly and that no one except he and Mytho could ever really love her, when the Raven mentions wishing to see her wedding day, Rue only gives a genuine smile of gratitude and thanks him. Anyone who has ever been emotionally abused and manipulated will recognize that smile immediately.
- Rue's sheer incredulity when Autor says he's fallen in love with her enough to sacrifice his own life is heartbreaking in its own right. The years of the Raven's abuse have really worn her down to the point where she cannot fathom anyone but Mytho and The Raven ever loving her. Even her laughter in that scene doesn't make it any less sad because of what she says afterwards: "What you're saying can't possibly be true!"
- Rue's dance with ravenified!Mytho is absolutely heartbreaking. He's literally a monster, she made him that way, and she dances with him and remembers how she fell in love with him only to hurt and transform him against his will. It's beautiful and scary and tragic all at the same time.
- The last few episodes of the second season will bring anyone to tears. Namely Rue's backstory, Rue's confession and giving herself up to save Mytho, Mytho choosing Rue over Ahiru, and Ahiru giving Mytho up willingly.
- The extras on the US DVDs show Jessica Boone and Luci Christian tearing up, as well.
- Episode 25 has Duck being driven into complete despair, wondering if everything is her fault, and, at Drosselmeyer's prodding, walking off to drown herself in the lake.
Duck: I thought I was doing my best for Mytho's sake...but was it for my own sake? Just like Mr. Drosselmeyer said...
- What makes it worse is a heartbreaking shot of her crying underwater as she sinks.
Duck: Fakir, the pendant won't come off! ...Oh, this is my fault! Because I'm thinking that deep inside my heart I don't want the story to ever end!
- And later, she tries to tell Fakir that it is her fault.
- Drosselmeyer doesn't just send Duck into the Lake of Despair with his own power, he forces Fakir to do it. Leaving poor Fakir to yell and scream in protest as he's forced to write out Ducks tragic fate.
- Fakir and Ahiru's pas de deux in episode 25. You can cry just hearing the music featured during that scene. It's worse when you realize it's The Last Farewell from Prokofiev's Romeo and Juliet. The last scene between Romeo and Juliet before their deaths.
- Duck's goodbye to Uzura in episode 25. The music sells it, as does Uzura crying.
- The scene in episode 25 where the final heart shard is restored, especially Fakir's narration as it happens. And the music.Mytho: I never doubted you would come for me. The feelings you returned to me...The feelings of love, affection, sorrow, loneliness...All of my feelings told me that Tutu was sure to come for me.
Fakir: Tutu quietly gazed into the prince's eyes, then took off the pendant. The final heart shard was shaped like a pair of fluttering wings. Wings for the people who had spent a comfortable time inside the story to leave the nest with...
Fakir and Tutu: Wings for the prince to return to himself with.
- In episode 25 when Tutu becomes a duck forever, Mytho bows to her in awe. This music makes it.Mytho: You were... That something so small and fragile accomplished this... Thank you, Princess Tutu.
- Rue's dance in the Depths of Despair, set to The Dying Swan.
- When Mytho tells Tutu he wants to make Rue his princess, she sheds tears, knowing her love for Mytho is destined to go unrequited.
- Episode 26 has Fakir's narration as Duck dances to save the town. Not only is the character crying, so is the actor.
- Episode 26 also has everyone crossing the Despair Event Horizon despite all that's been done, set to tear-jerking music as crow feathers cover the characters.Mytho: At this rate I'll just be letting them steal my heart... So, I really have no choice after all than to seal him away by running my sword through my own heart again?Fakir: No... It's not like that, Mytho! ...Is it true, then? Do I really not have enough power to rewrite the story?Duck: Without the pendant I'm just a duck... Just a duck without any power... Just a duck who can't do anything!Drosselmeyer: That's right! Even though you transcended your duck's place in the world and tried so hard!
- Eventually it reaches the point where Mytho decides to shatter his heart once more.
- Duck's empty room when Gold Crown Town is restored to normal.
- Pike and Lillie forget all about Duck, who never even got to say goodbye to them.
- The final scene of the series is an angelic Duck, sadly fitting as human Duck is gone for good.