The entirety of the sequence of Vizla giving a lecture to a classroom of 8 year-olds about how to kill people with a kukri and the various ways people die all over the world.
When Blut's remaining guards abandon him rather than face Vizla who already murdered a few dozens of them, they pass Vizla on the way out and point out which room his target is in.
More of a bit of meta humor, considering the genre of hitmen and revenge stories: Duncan comes across a woman with a dog that she offers to sell to him. After a minute's consideration, he comments that "I don't need a dog." Then he ends up getting the dog anyway, has several heartwarming scenes of him taking care of it, and then accidentally shoots it when coming out of a PTSD dream.
Sindy's petulant "Oh no, you killed him!" when the Kevlard victim gets shot.