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- The aforementioned Joan Blondell, especially in Footlight Parade.
- Moneypenny from the James Bond movies. She only fits the "cracking wise" personality though.
- Grosse Pointe Blank might be considered a subversion, as while John Cusack's character has such a secretary (played by his sister, Joan), he is an amoral assassin. Moreover she herself is an effective operative, rattling off info from dossiers, dressing down people over the phone, and nonchalantly dousing the office in gasoline when it's time to move on.
- Janine Melnitz of Ghostbusters (1984). Trope Codifier and snark queen extraordinaire. She can throw it back at Venkman as hard as he can dish it out, saying she'd quit better jobs. Then again, being the babysitter to three Mad Scientist types would test anyone's patience.
- Janet, Russ' long-suffering secretary in Disney's The Kid. She deals with Russ's frequently unreasonable demands with healthy doses of snark and sarcasm.
- Pamela Finkelstein from UHF certainly qualifies. Her short fuse and annoyance are Justified because she really signed onto the station to be a news reporter and got shunted into clerical work instead. George, being George decided "why not" when letting her cover news. Hilarity Ensues.
- The receptionist from the Irish short Na Fíorghael goes way beyond "sassy" into "wildly unprofessional." She's seen insulting the clients (in Irish) in her phone conversations, and despite not understanding her, she doesn't make much a secret with the furtive glances and the laughter. The clients take it upon themselves to learn enough Irish to rat her out and kick her out onto the street. Justified, though, because the kicker of the short is that it's all an act: she's not really a secretary.
- Connie in the Stephanie Plum series of novels. She has Mob connections, an impressive collection of weapons in her desk (on at least one occasion, she threatens to use one on lecherous boss Vinnie), and a "take no crap" attitude that serves her very well dealing with her colleagues.
- Sir John's clerk Mrs Pound from Darkness Visible is sassy enough to deliberately wind up Lewis when she has information he needs to prevent the end of the world. Of course she's going to give it to him, but not until he realises how damned hard it was to get...
- Vicki Lewis ("Beth") on NewsRadio reportedly feuded with Kathy Griffin because of her similar redheaded character on Suddenly Susan. It is worth noting that Griffin's character, while a complete ripoff of Lewis's from the standpoint of personality, is not actually a secretary.
Beth: Mention that there's a spunky redhead in this office.Joe: I don't know how to tell you this, but there's a spunky redhead in every office.Beth: Tell me about it.
- She shouldn't have been so upset about one character being like her character, so much as for most of The '90s approximately 80% of the shows on NBC were about single people working at a media outlet in a big city. They all had a character that was exactly like hers.
- Whenever Picard adopts the persona of Dixon Hill in the holodeck on Star Trek: The Next Generation, he gets his own Sassy Secretary to complete the genre trope.
- A rare male example: Dennis Finch (David Spade) on Just Shoot Me! (though he denies being a secretary, at least at first).
Finch: (answering phone) Blush magazine, underpaid phone monkey speaking.
- Oddly enough, Russell Dunbar (also Spade) has his own rare male example, Timmy, on Rules of Engagement.
- Mrs. Landingham and Debbie Fiderer on The West Wing. Most of the other secretaries get in on the snarking action occasionally, but the President's secretaries are the most consistent.
- Elaine on Doc Martin, to the point that Martin actually fires her in annoyance during the first episode (the rest of the town forces him to hire her back). Mostly averted with Pauline, though she really "wants" to be one.
- Veronica in dads. She takes a fair amount of abuse from her bosses, but gives back just as much.
- Legal secretary Carrie Heffernan in The King of Queens who is frustrated as hell at her lowly position in the firm, strives for advancement, studies by night, but is always overlooked.
- Donna to Harvey in Suits. She's the best secretary in the firm and knows Harvey more than anyone (it's revealed in a later flashback that they slept together once before either of them joined the firm). She's not afraid to call Harvey out on his bullshit and frequently listens in to the conversations in his office.
- Lolth has one. No, seriously, the dreaded Demon Queen of Spiders of Dungeons & Dragons has a Sassy Secretary, and Player Characters have to deal with her in the epic adventure Vault of the Drow (from way back in 1980). Seriously. When the players reach the room with the sole access to Lolth's throne room, they find a Type V demon (called a marilith in later editions, a very powerful six-armed female fiend with a snaky lower body) at a messy desk who insists they sign in before they confront Lolth, and then wait while she finds the ledger. (Craziest part? If the PCs comply, she lets them in with no argument! If they don't wait, they'll have a much harder time getting in, not just from fighting her, but triggering an alarm and increasing the probability of random encounters. Doing what the secretary says lets them conserve their power for Lolth herself, who is no pushover.) No explanation is given for this and if the players ask, the demon just says "Lolth told me to". (Maybe this demon is just being punished for some slight by being given a job that chaotic fiends would likely hate, but that's just conjecture.)
- The demon secretary appears in a Nodwick strip much later that parodies the adventure, resembling a more traditional type, a coffee addict using a laptop. She's sassier and ruder than the one in the source, and not all-too good at her job, more occupied with playing solitaire on the computer than dealing with the heroes, who eventually sneak past by leaving dummies in the waiting room.
- Eva from Grim Fandango.
Manny Calavera: It's my boss's secretary, Eva.Eva: It's my boss's whipping boy, Manny.Manuel Calavera: Any messages for me?Eva: No, calls stopped coming for you the day you left. They're STILL sending you that lingerie catalog, though.Manuel Calavera: Busy as ever, I see.Eva: I'd have more work to do if you had more clients.
- In The Space Bar, the main character has a PDA with a "female" AI called Zelda that emulates one of these. She snarks at him whenever he does something stupid (like trying to operate his fingerprinter without opening it), and sometimes makes sarcastic quips to him about the information he reads or the people he's interrogating.
- Mallory from Grand Theft Auto IV is a good example; often taking the wind out of Roman's sails when he tries to boast or scheme.
- Laura from Dr. Katz, Professional Therapist qualifies, but she's extremely rude and useless as a secretary. She doesn't take messages, and refuses to bring Dr. Katz coffee.
- In the My Little Pony: Friendship Is Magic episode "Hearths Warming Eve", Chancellor Pudding Head's secretary Smart Cookie is portrayed as quite the snarker.
- In The Real Ghostbusters, Janine started out just as sassy as her movie incarnation, and even sported a bright red punk-style hairdo, but her incarnation in Extreme Ghostbusters wasn't nearly as much so.