When his transformation kicked into over-drive, and his first reaction was "I've been double-crossed! I've been framed!" As if he'd correctly deduced that someone else was turning him into a donkey. Like you'd really think that way in that situation.
"What's he think I look like? A jackass?"
You sure do!"
Even the coachman's infamous "...as BOYS!" line is pretty funny to watch when one is older.
What was the Tear Jerker moment of the movie, reverses itself when the now-real Pinocchio asks Geppetto why he's crying, who answers with " because you're dead, Pinocchio. Now lie down."
However, there's a bit of Fridge Horror in that moment; Gepetto doesn't seem surprised to hear Pinocchio's voice, which could imply that he's been hallucinating in his grief, and doesn't realise that the voice is real this time.
When Honest John and Gideon are performing a fake diagnosis on Pinocchio to get him to go to Pleasure Island.
Honest John: Oh my, this is serious. (cut to Gideon's notepad, which is nothing but scribbles)
The first time Monstro sneezed, and Jiminy gave a rather understated "Gesundheit." Made even funnier considering it occurred during an otherwise very dramatic moment. The staff admitted during the premiere, that line got the biggest laugh of the movie.
In "I've Got No Strings":
Pinocchio: I've got no strings to hold me do—(falls down the steps)
After the clocks scene, Gepetto still has to check his pocket watch for the time.
During the fishing scene, Figaro slaps one of the tuna in the face after it lands in the trough.
And when he puts another one in, the fish's tail smacks Figaro in the face.
After the annoying clock scene (a CMOF in itself), Jiminy sees a bright light coming through the window.
Jiminy:Now what's up?
Admit it, Stromboli is one of Disney's most hilarious villains ever, even during the scene where's locking Pinocchio up in a cage.