- Geppetto's Adult Fear scene when he walks the raining streets in search of his puppet-son who never returned home from school is hard to watch. Geppetto wanted this little puppet boy to be real so he could have someone to love and care for, and after one day, he's already lost him.
Geppetto: [agonizingly] What could have happened to him? Where could he be at this hour? [determinedly]...I better go out again and look for him.
- Geppetto walking through the pouring rain at night, his forlorn cry for his boy drowned out by a crack of thunder. He looks so helpless.
- Also when Jiminy discovers the pen where the Coachmen keeps the donkeys that can still talk.
Donkey: [sobs] I wanna go home to my mama!!!
- Made all the more hauntingly tragic since unlike so many examples here there is no uplifting conclusion to this. This is the last we see of those poor boys as they are likely sent to some horrible fate by the Coachman for the rest of their lives. The endearing designs and voices used for the donkeys doesn't help one bit.
Jiminy: Boys? So that's what...PINOCCHIO!!! [later] I hope I'm not too late!
- This line from Jiminy when he discovers this atrocity. He's abandoning Pinocchio and his duties as conscience, but when he see what the boys on Pleasure Island end up becoming, he rushes back to save Pinoke.
Donkey: [All the talking donkeys start protesting] Please, please! I don't wanna be a donkey!
- Lampwick's transformation. Especially the part where he begs Pinocchio for help, even telling him to call Jiminy not realizing that Jiminy, having learned of the island's curse, was already on his way but would not be there in time to save him.
- Bit of Fridge Horror/Fridge Tear Jerker in it when you realize: those boys' parents will never know what happened to their sons. All they will ever know is that one day, their little boy didn't come home...
- The boys being stripped of their humanity as their clothes are torn off and all they can do is bray helplessly, realizing that they are likely doomed to live the rest of their lives as otherwise ordinary working animals, with no way of turning back or of anyone ever realizing that they had once been human.
- Lampwick crying for his mom. He may be a jerk, but still... poor kid.
- All the boys who can still talk pleading the Coachman to let them go and to change them back, only for their pleas to fall on deaf ears.
- When Geppetto see's Pinnochio's Donkey ears and tail and asks what happened to him, the brief moment when Pinnochio timidly tries to explain everything very likely resonates with many people's childhood moments when they had to try and tell their parents that they did something bad.
- "Pinocchio... Swim for shore! Swim for shore! Save... yourself..."
- Even decades after the ending became common knowledge, Pinocchio's apparent death scene still contributes handsomely to the well of tears Disney sought to reap from its audiences.
- "When You Wish Upon A Star". So happy that it brings tears.
- "Solid gold, too! Oh I think it's swell."
- When Pinocchio is locked in a cage in Stromboli's caravan.