In Thunder Cats: Dogs of War, Lion-O tries to get the Doberlord court-martialed for War Crimes, only for Lion-O's nemesis, Mumm-Ra, stab the latter through the chest from behind, when you consider how the mad dog enslaved and or massacred millions, if not billions, universal-wide, it'd be impossible you to have any kind of sympathy for the bastard.
The Comedian, although that had nothing to do with the (primary) motivation behind his murder. By the end of the comic, some readers feel some sympathy for him. But he's still an asshole.
Moloch, though he hadn't been an asshole in years.
Gerald Grice. The plot needed to show Rorschach violently murdering someone, to establish the full onset of his insanity. Grice being a murderer himself makes the story a very solid Black and Grey Morality type.
As bad as Doctor Doom is, King Vladimir Vassily Gonereo Tristian Mangegi Fortunov, the former ruler of Latveria, whom Doom deposed and killed, was a far worse tyrant by most accounts. The same goes for anybody who successfully overthrows him; every time it happens and he later seizes back his power, the citizens are always glad to have him back.
The Punisher usually goes after some rather nasty people. The people he kills tend to be even worse. At least one (if not two) exception was the Punisher being unwillingly wacked out of his skull on drugs.
Most people who get beat up by The Hulk usually have it coming. Bruce Banner just can't stop running into assholes who go out of their way to make the poor guy mad.
Most of Johnny's victims are this. Or are implied to be people like this. Or hung out with people like this. Or stood too close to people like this (i.e., around two kilometers). Come to think of it, Johnny doesn't really discriminate once he's gotten going, but it takes a soon-to-be Asshole Victim to trigger his homicidal rampages... Mostly. Most of the people he takes back to his Torture Cellar (who will be dead pretty soon) are prime examples however. Or at least implied to be.
Jimmy, Johnny's creepy fanboy/admirer, murders several innocent people for no reason other than being like his "hero", Johnny. But without a doubt his worst crime is raping and killing an innocent girl, just because she looks like another who dissed him in school. He's the title character with no sympathetic qualities. When the reader witnesses his brutal murder at Johnny's hands, not a single tear is shed.
Tommy Monaghan, the titular protagonist of Hitman, only takes contracts out on those he considers to be "bad" people.
In the backstory of Kingdom Come, Magog kills the Joker while he's in police custody. This is The Joker, and he was arrested because he went on a rampage in the Daily Planet offices and killed 75 people — including Lois Lane. When Superman protests, the public sides with Magog for this very reason. This kind of mindset is gradually deconstructed, however. The public deciding that it's okay for heroes to kill someone just because people think they're an asshole eventually leads to an entire generation of out-of-control and reckless heroes who don't hold back for anything — including innocent bystanders. But still...
Speaking of the Joker, he kills Sheila Haywood, Jason Todd's mother in A Death in the Family. She'd lost her medical license for performing back-alley abortions (one of which killed a patient) and was embezzling from her aid agency. She turned over her son to the Joker to save herself (instead of using her gun), who decided to kill both of them anyway.
In the first Deadshot miniseries, Deadshot—at the time a Boxed Crookworking for the government—went on an unsanctioned spree of torture, maiming, and murder. However, since every one of his victims was complicit in the kidnapping, rape, and murder of Deadshot's young son, neither his boss nor the readers cared all that much.
Anyone the Secret Six killed in their eponymous series for the exact same reason as the Punisher example above.
As he's moved to being a more heroic and less serious character, Deadpool often ends up with these kind of targets when he's working as an assassin.
Much like for the Punisher, the people Spawn kills usually are douches, making it easier for the fans to cheer as he dismantle them gruesomely.
When she kills Zander Rice, she puts away the claws and beats him for ten minutes in a truly brutal No-Holds-Barred Beatdown. Rice tortured and both physically and emotionally abused Laura all her life to that point,note Forcibly activating her Healing Factor at age seven with fatal dosages of radiation, removing her claws one at a time for coating in adamantium without anasthesia, torturing her into responding to an olfactory trigger that sends her into a berserker rage whenever she smells it and using it to force her to murder the only two people at the Facility who cared about her, intentionally leaving her behind to be killed on a mission out of spite, and placing her in the "care" of the sadistic Kimura, among other things. And he only did this because Wolverine killed his father and Laura was created using his genetic material. so it makes the scene far more satisfying to read than watching someone get pounded into an unrecognizable pulp would normally be.
Martin Sutter, as well. Although he didn't take a direct hand in her brutal upbringing that Rice did, he fostered the environment which enabled him to do so. Sutter summarily rejected every suggestion or warning Sarah Kinney made, and his entire motivation for the project was sheer greed. He never once had misgivings about what he and Rice were doing, and it's hard to feel a shred of sympathy for him when Rice manipulates him into turning over full control to him, and then sends X-23 to kill him.
Happens in quite a few EC Comics, as often someone invokes a Karmic Death by driving another person to kill them.
The Batman story, Dark Victory involves a Cop Killer called the Hangman. Among the victims are the Corrupt Cops from Batman: Year One: former commissioner Gillian Loeb, Gordon's former partner Arnold Flass, SWAT Lt. Branden, and SWAT officer Pratt. While Flass and Branden only appear in the mini as corpses, both Loeb and Pratt each get a moment to further cement their asshole behavior before they die: Loeb swings by recently promoted commissioner Gordon's office to gloat about Harvey Dent's transformation into Two-Face and Chief O'Hara being the first victim of the Hangman (and not-subtly imply he's gunning to get his job as police commissioner back) and Pratt tries to shoot Batman in retaliation for Batman punching him through a wall.
Caballistics, Inc.: Demon Jenny hits the bars a few times in search of fresh victims, one of whom was apparently a serial rapist and another one a child molester. Justified because she mentions that she feeds on human sin.
100 Bullets has this trope as the impetus for its plot: ordinary people, selected seemingly at random, are given a chance to get even with someone who has done them a great wrong and get away with it. Many take the chance, and many other assholes are similarly dispatched even if they aren't the primary target. Examples have included...
A pair of murderous, racist Dirty Cops perfectly willing to kill children.
Arawn: Math and Engus were two vicious and bloodthirsty warlords that betrayed their own kin for ultimate power, murdered several innocents and were depraved rapists (the former raped his brother's wife and the latter was a pedophile). When Arawn finally becomes King of Hell, inflicts eternal torture upon them by having Math to be quartered forever and Engus beheaded and forced to look to a mirror for all time with his eyes stitched open. Even with such brutal punishments, Arawn feels they got off too easy.
Deathstroke's bastard Titans went up against these types of villains on most of their missions, including a crime syndicate that manufactured a highly addictive drug literally made from children, a serial child molester with an hypnotic voice, the man who remorselessly murdered Tattooed Man's son, and the leader of an organization of underground human death matches where the fighters are experimented on and mutilated before they fight. This was on part of writer Eric Wallace's attempt to make the team of self-admitted villains seem better in comparison and thus earn sympathy from the readers. For a variety of reasons, it didn't work and fans hated the Titans just as much as their enemies.
Subverted in The Mask comics: the fates "Big Head" inflicts on people are often so extreme that even when the victims are assholes, it's usually more horrific than cathartic.
Notably averted in Tom Kingís Mister Miracle (2017) run. Orion acts like a total Jerkass to Mister Miracle and Big Barda for most of the story, culminating in trying to have the former executed out of fear he may be infected by the Anti-Life Equation. Yet when Darkseid brutally kills him, Miracle is horrified and nearly collapses in grief; Orion may be a total dickhead, but heís also Scottís brother and Scott will always love him as one.