A couple of gentlemen, whose last names are X and Y, exchange polite banter, in which they frequently call each other "Mister X" and "Mister Y." There is usually some kind of Theme Naming going on between the two.
- Mr. Shytte and Mr. Pysse, two royal guards attending Queen Gloriana, in The League of Extraordinary Gentlemen.
- Mr. Schlubb and Mr. Klump of Sin City, better known to those not in their habit of referrating to each other by their respectablive patronism as Fat Man and Little Boy.
- In Harry Potter and the Methods of Rationality, Crabbe and Goyle are faithfully following minions clichés and call each other "Mr. Crabbe" and "Mr. Goyle".
- Those Two Bad Guys in Diamonds Are Forever always call each other Mr. Wint and Mr. Kidd.
- Creepy Twins Dr. Dee and Dr. Dum from Alice, Syfy's version of Alice in Wonderland.
- The villains from Dark City do this — although it's the only names they've got. Mr. Book, Mr. Quick, Mr. Hand, Mr. Wall...
- Race for the Yankee Zephyr. Played for Black Comedy when Mister Brown introduces two of his thugs as Mister Broken Teeth and Mister Ruptured Spleen.
- While not used in the film itself, some posters for Men in Black took note of the fact that the two leads are Mr. Jones and Mr. Smith.
- Those Two Bad Guys in Neverwhere: Mr. Croup and Mr. Vandemar.
- Those Two Bad Guys in The Truth: Mr. Pin and Mr. Tulip.
- Those Two Bad Guys in Hamish X: Mr. Candy and Mr. Sweet.
- Those Two Bad Guys in Peter Straub's story Mr. Clubb and Mr. Cuff.
- Those Two Guys in American Gods: Mr. Ibis and Mr. Jacquel. Furthermore, the Spookshow agents have names like Mr. Town, Mr. Wood, Mr. Stone, Mr. Road and Mr. World, and they tend to introduce themselves like this.
- The League of Gentlemen, as a Shout-Out to Diamonds Are Forever, has a pair of surveyors who call each other Mr. Wint and Mr. Kidd.
- Whose Line Is It Anyway?: Wayne Brady and Brad Sherwood do this during their vaudevillesque routines.
- M*A*S*H: The episode "Big Mac" had the 4077th rehearsing for the impending visit of Gen. MacArthur, with Radar playing the General. Inspecting the troops, he first comes to Hawkeye and Trapper, who introduce themselves:
Hawkeye: Captain Sodom and Captain Gomorrah. He's Gomorrah.
- Mr. R and Mr. G, Those Two Bad Guys in the Shakespeare & Hathaway - Private Investigators episode "This Promised End".
- The song "Mister Gallagher and Mister Shean" by Ed Gallagher and Al Shean. Probably the Trope Maker; certainly the exchange "Absolutely, Mr. Gallagher?" — "Positively, Mr. Shean" became a popular meme.
- In The Golden Apple, shifty stockbrokers Mister Scylla (played by the same actor as Menelaus) and Mister Charybdis (alias Hector) do a pastiche of the Gallagher and Shean number.
- In 1776, when John Adams and Thomas Jefferson are arguing over who will write the Declaration of Independence they refer to each other as Mr. A and Mr. J.
- Classic Vaudeville Schtick: e.g. Mr. Bones and Mr. Jones, done by such as... Mr. Gallagher and Mr. Shean.
- Fur Fighters has two villains, Mr. Grr and Mr. Argh, a parody of Mr. Wint and Mr. Kidd who also happen to be conjoined twins.
- Dawn Of War II Retribution: Ork Freeboota Kap'n Bluddflagg and his Number One Miss'ta Nailbrain do this. A lot. To the point where the Ork campaign is essentially an extended "Mr X and Mr Y" gag.
Kaptin Bluddflagg: Miss'ta Nailbrain, it looks ta me like dese Eldars want ta run off wiv all dese nice shiny bitz an' gubbins.Mr. Nailbrain: Well Kap'n, dat's jus' rude dat is. An' after we came all da way down 'ere ta steal 'em! Wot should we do sir?Kaptin Bluddflagg: Well Miss'ta Nailbrain, I reckon' we should kill dem. 'Orribly of course.
- Sam and Fuzzy has several instances of these. The two highest ranking assassins in the Ninja Mafia have names like this which often pair together. The first we see are Mr. Blank and Mr. Black. The two most recent are literally Mr. X and Mr. Y.
- Mr. Wink and Mr. Fibb (parodies of Wint and Kidd) from Codename: Kids Next Door.
- Mr. Doe and Mr. Cardholder, the mysterious OSI agents, in The Venture Bros.
- Mr. Touch and Mr. Go on Teenage Mutant Ninja Turtles (2003).
- Mr. Black and Mr. White from Johnny Test. Interestingly enough, Mr. Black is white, and Mr. White is black.
- Mr. White and Mr. Whyte from Ruby Gloom.
- One Looney Tunes cartoon had Daffy Duck and Porky Pig as Mr. Monday and Mr. Tuesday, respectively.