The Spider-Gangnote Miles Morales / Spider-Man II, Gwen Stacy / Spider-Woman, Peter B. Parker / Spider-Man, Mayday Parker, Pavitr Prabhakar / Spider-Man India, Hobie Brown / Spider-Punk, Margo Kess / Spider-Byte, Peni Parker & SP//dr, Peter Parker / Spider-Man Noir, Peter Porker / Spider-Ham
Spider-Societynote Miguel O'Hara / Spider-Man 2099, Jessica Drew / Spider-Woman, Ben Reilly / Scarlet Spider, Lyla, Peter Parker / Spider-Man (E-67), Peter Parker / Spider-Man (Earth-13122), Malala Winsdor / Spider-UK, , Patrick O'Hara / Web-Slinger, Charlotte Webber / Sun-Spider, Max Borne / Spider-Man 2211, Spider-Cat, Peter Parker / Spider-Man (E-1048), Peter Parker / Spider-Man (E-26496), Peter Parker / Spider-Man (E-751263), Pter Ptarker / Spider-Rex, Peter Parkedcar / Spider-Mobile, Tarantula, Peter Parker / "Plushie" Spider-Man, Cyborg Spider-Woman, Spidercide, Spider-Canada
Supporting Charactersnote Jefferson Morales, Rio Morales, George Stacy, Peter Parker / The Lizard, The Mary Janes, Ned Leeds, Yuriko Watanabe, Mary Jane Watson-Parker (E-616B), Maya Prabhakar, Gayatri Singh, Inspector Singh, Ganke Lee, May Parker, Ben Parker, Aaron Davis, Rio Morales (E-42), Jefferson Morales (E-42)
Antagonistsnote The Spot, Armadillo, Aaron Davis (E-42), The Prowler (E-42), Adrian Toomes / Vulture, Captured Anomalies