Diego Marquez
- The Ace: It's implied Diego's treated like this. Due to how he rescues animals not just from his home continent, but also, from all around the world too.
- Costume Evolution: In Season 4, he wore a red short-sleeve hoodie with a red and orange shirt underneath, black cargo shorts, white socks and red and black sneakers.
- Free-Range Children: He goes on animal rescue missions on his own.
Baby Jaguar
- Suddenly Voiced: In the original show from seasons 3-4, he only talks through meows; in the spinoff and in later seasons of the main series, he talks verbally. Justified as Diego can understand animal language.
Alicia Marquez
Diego's sister and an animal scientist.
- Costume Evolution: In Season 4, she wore a blue short-sleeve hoodie with a light blue undershirt, a blue bracelet, khaki cargo shorts, white socks and blue shoes.
Daisy Marquez
Alicia and Diego's oldest sister.Sabrina and Nico Marquez
Daisy, Alicia and Diego's parents.Rescue Pack
- Voiced by: Keeler Sandhaus (Seasons 1-3); Kyle Brenn (Season 4)
Diego's version of Backpack who can transform into many types of things.
Click
- Voiced by: Rosie Perez
Diego's camera which he uses to find the animals in trouble.
The Bobo Brothers
- Voiced by: Jose Zelaya (Seasons 1-3); Andres Filipe Aristizabal (Season 4)
Two twin monkeys who often cause trouble for Diego.
- Catchphrase: "Bobos, Bobos!" Also "Whoops, sorry!" when stopped.
- Phrase Catcher: Saying "Freeze, Bobos!" stops them.
- Suspiciously Similar Substitute: To Swiper, since they tend to pop up at least once per episode to cause trouble, though at least you only have to say it once to stop them instead of thrice.
