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Makoto will be a magical girl variant of the Undercover Model trope.In that she only got into the idol business to find Cure Lavies. Unless, of course, she was an idol before meeting Davi.
Ai-chan is Princess Marie-Ange in disguise.Given that the Princess has now been revealed to have pink hair and blue eyes, the probability of this has just doubled.
There will be four more villains named after the four remaining sins.
One of the Cures will be corrupted.And will be called Cure Joker.
Mana is a Tokusatsu fangirl.Though much more secretive about it than Yayoi/Cure Peace.
Joe is from the Trump Kingdom; either a knight or a member of the royal family.His name spells out 'Joker' when written in full katakana, he's definitely done something to help Mana transform into Cure Heart and was referring to her by her attack name even before she earned her powers. He must be from the Trump Kingdom, working to help Sword by getting her more allies and/or watching over her.
Cure Sword was actually part of a different Precure team before the start of the series.But something bad happen to them and that's why she was cold towards Cure Heart.
Joe will refer to all four Cures by their attack names.He called Mana "My Sweet Heart" twice in episode 1, so it's quite likely he will do the same for the other three, calling Rikka "Twinkle Diamond", Alice "Rosetta Wall", and Makoto "Holy Sword".
Mana will have a Broken Pedestal moment after finding out Makoto and Cure Sword are one and the same.
Rikka's gonna be the Butt MonkeyGetting screwed stopping people from taking advantage of Mana's kindness in the process.
Joe is actually a PrecureHe ends up being like the Sailor Starlights and is actually a female in a male's body who transforms into Cure Joker.
Davi is the managerIt's not like mascots turning into humans is anything new.
There will be a Monster of the Week created from the heart of one of the CuresAlmost certainly created while she's in her civilian form, and likely the result of a misunderstanding or argument between two or more of the Cures. So far the most likely candidate seems to be Rikka (due to having the least focus).
Joe is the Princess in disguise.Wouldn't it be effective for the Princess to disguise as a male, then surreptitiously hand out the Cure Lovies?
Joe is a fairy from the Trump Kingdom.Seeing as Davi can turn human, perhaps Joe is also a fairy who escaped from the Trump Kingdom's destruction. Except instead of helping the Precure transform, Joe's job was to give the Lovies to the people who he saw fit to protect the princess (in other words, he "knighted" them).
The princess has been brainwashed.The ...and Crazy is optional.
One of the Precures from Makoto's team is Not Quite Dead......and is in fact working for the enemy. In the OP, when the lightning flashes in front of (what is believed to be) the enemy King you can see a small dark silhouette that seems like a long-haired girl.
The Princess escaped to the human world.The strange light that appears after every battle and fixes all the damage is her doing. It's likely some skill exclusive to the Royal Family (called "Light of Restoration" or something like that.)
Speculation of suits for possible additional Cures...
Cure Rosetta will go berserk
Joe Okada is the Big Bad
Regina will be Doki Doki's equivalent to Joker.She may look goofy so far, but wait until she starts appearing more often.
Guesses for Cure Ace's identityMost people seem to think she'll be either Princess Marie-Ange, possibly having been reborn as Ai-chan, or Regina after going through a Heel Face Turn. I'll Take A Third Option here and say it's a brand new girl, which would be a first for Pretty Cure.
The mysterious blue birdSo far, whenever the girls recover one of the Royal Crystals, a blue bird is shown to be observing them. There are a few possibilities for who or what this mysterious bird really is:
Regina is a Brainwashed and Crazy Princess Marie-Ange...Who will undergo a Heel Face Turn and become Cure Ace.
Cure Ace's color schemeEpisode 14 introduced the Royal Crystals as five different colored items, each one appearing to correspond to an existing cure's color scheme except the fifth one, which is red. Therefore, it is very likely that Cure Ace's color scheme will be red.
There will be atleast two Sixth Ranger PrecuresIf Cure Ace wasn't a part of the Trump Kingdom's Precures, a brainwashed Precure will appear and make a Heel Face Turn.
Regina and Ai-chan are both parts of Princess Marie-AngeMore specifically, Regina is actually Marie-Ange's Jikochuu (after all, Marmo did almost make one from her in episode 7), who was made to believe that she was always his daughter. (And if Regina is indeed Ace, this would sort of explain why she looks quite a bit like Ange when transformed.) Ai-chan, on the other hand, is the incarnation of all of the princess' positive energy, sort of like Chibi-Chibi in the Sailor Moon anime. Only when the two merge will Ange come back.
Regina is Marie-Ange's Long Lost RelativeHer sister, specifically. Yes, this does mean King Selfish is Marie-Ange's father. It's Royally Screwed Up, like in Suite Pretty Cure♪
Related to the Cure Rosetta going berserk WMG above, she will eventually use one of her usually defensive attacks in an offensive way, a la Cure Mint.