As this is a Death Trope, all Spoilers will be unmarked ahead. Beware.
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Anime & Manga
- In Legend of Galactic Heroes, when Yang's old friend and mentor figure of sort Alexander Bucock gets killed during a desperate battle he has started to give Yang enough time to organize his own troops.
- In Muhyo and Roji, George Schuyter had quite a few of them, leading to him getting a reputation among the Magical Law Society for getting his partners killed. As a result, he acquires the sword Shinokuni and the envoy Amon and sets out to practice magical law alone, but this doesn't do much good against Vector.
- Smax and Stochastic Fats from Top 10. Naturally, he has a hard time warming up to his new partner, Toybox. A similar situation occurs later in the series, when Girl One dies. Her partner, Irma Geddon, considered her family and was very close with her. As such, she treats her new partner, Joe Pi, with extreme hostility for a while.
- At the beginning of The Simping Detective, Jack used to have a partner named Cheyne. He and Jack killed the brother of someone very important. Jack is a tad more resourceful than Cheyne.
Films — Live-Action
- Foxxy's partner in Austin Powers in Goldmember was killed by the eponymous villain.
- Det. Sykes' previous partner in Alien Nation.
- In Who Framed Roger Rabbit, Bob Hoskins plays a private investigator who developed a sour disposition, a drinking problem and a hatred of cartoon characters after the murder of his brother.
- In OSS 117: Cairo, Nest of Spies , the plot is set in motion by the death of OSS 117's partner, and subsequently each mention of Jack causes OSS 117 to look wistful while Ho Yay-laden flashbacks appear on screen. Of course, He's not really dead. Since the whole film is a parody, this is Played for Laughs.
- Inspector Lee's previous partner in the Rush Hour canon was murdered by the Big Bad of the second movie.
- Part of Kung Fury's backstory. We in fact see Kung Fury's partner getting killed in flashback.
- Dirty Harry: Pretty much all of Harry Callahan's partners end up dead or in the hospital, as he notes.
- In The Maltese Falcon, Sam Spade notes that one has to avenge one's dead partner, regardless of one's personal feelings towards the partner.
- Split Second: Stone lost his old partner Foster to the monster when they were both investigating the sewers. When Durkin asks Da Chief if Stone has Survivor's Guilt, he says that it's just plain guilt; Stone happened to be screwing Foster's wife at the time.
- Vincent's partner, Ernie, in Anonymous Rex is dead. Casual Rex is a prequel, leading up to how that happened.
- The death of Nathan's partner in Every Demon Has Its Day is what drove Nathan to leave Texas and go home to Dogwood County.
- This is part of Officer Borsch's backstory in the Sammy Keyes books.
- This is part of Hardison's character background in the Leverage episode "The Bank Shot Job".
Cop: I think we should go by the book on this one.
Hardison: The book?! The book got a good man killed! (turns away, apparently overcome by emotion)
Parker: Ex-partner. Probably shouldn't mention the book again. Or propellers.
- Randall and Hopkirk (Deceased) and its remake both involve a cop working with the ghost of his deceased partner.
- In one episode of Castle, this trope was played with like mad for Esposito's backstory. His then-partner Ike Thornton was killed by a mob boss they were investigating. Except Thornton faked his death because there was a Dirty Cop informing on them, and kept investigating as a private citizen.
- In Tensou Sentai Goseiger: Gosei Blue's partner, Gosei Green, was killed in action before the series began, which is why he's the only team member from the Seaick tribe.
- In The X-Files, Mulder has this moment when paired with rookie Alex Krycek during season two. Scully has her moment when paired with John Dogget in season eight. Subverted in the fact that the other wasn't dead, only missing.
- Miami Vice: Crockett's first partner falls afoul of a car bomb early in the pilot.
- Mike Logan (Chris Noth) from the early run of Law & Order had an unfortunate tendency to lose partners. One got shot and partially crippled. Another got shot and killed. The third outlasted Logan—yet still died years later.
- Law & Order: UK: DS Ronnie Brooks' backstory includes the fact that one of his partners was killed. What makes this worse is that it's his current partner DS Matt Devlin who accidentally reminds him of this—a year before Matt himself was killed in a drive-by shooting. However, Ronnie seems to subvert this trope, as neither of these tragedies have prevented him from forming good relationships with his subsequent colleagues—he got close enough to Matt to view him as a surrogate son. It was Matt's replacement Sam who seemed to be reluctant to develop a friendship. But when yet another friend of his (never his partner, but they knew each long enough that this trope applies) was killed off, this time, it did appear to have an adverse effect on his ability to bond with Sam's replacement.
- Adam-12 had Officer Pete Malloy, who was about to quit the force because his last partner got killed, get paired up with rookie Jim Reed. Malloy is... less than enthused by this. Later in the series, we also see that he had a good friend that went to the Police Academy with him who was killed.
- Detective Madsen in J. J. Abrams' Alcatraz is like this at the beginning of the series, though we actually see the incident in question before we see her refusing to take a new partner in the next scene.
- Jim Ellison in The Sentinel; slightly different because his new 'partner' isn't a police officer at all, but an anthropology graduate student.
- More like Undead Partner in Witchblade season one. Sara Pezzini's partner Danny Woo is murdered by a mafia hitman in the pilot, but the Witchblade lets her interact with his ghost and he follows her around dispensing advice for the remainder of the season.
- Det. John Munch lost his partner in Homicide: Life on the Street. It still haunts him as of Law & Order: SVU.
- Total Recall 2070: Agent David Hume's first partner is killed by a sabotaged Beta-class android who later jumps off a building. He is then partnered up with an Alpha-class android detective.
- Frank Reagan of Blue Bloods has a Dead Canine Partner, a police dog named Greta he'd handled as a young K9-unit officer. She was killed by a criminal Frank sent her after: an old regret that helped motivate him, decades later as Commissioner, to invest extra effort into exonerating another police dog that was falsely accused of biting a child.
- In Mortal Kombat, Sonya Blade's backstory is that she is pursuing Kano because he killed her partner.
- Ace Attorney Investigations: Miles Edgeworth:
- Jacques Portsman likes this trope so much that he invoked it personally. Poor Buddy Faith.
- Later (which is actually earlier, it's a flashback,) Detective Badd suffers this. Showing him evidence relating to his partner's murder is one of the few things that make him actually sentimental.
- Buzz Lightyear of Star Command has this with Warp Darkmatter, Buzz's former partner. It's later revealed that his Warp didn't die, and was on Zurg's payroll all along.
- In C.O.P.S., Sundown's old partner Johnny Yuma didn't die... he went heel. As Bulletproof puts it, "Yuma never forgave Sundown. I think Sundown never forgave himself."
- Parodied on The Critic, Jay reviews a Dirty Harry expy cop film in which the Cowboy Cop is assigned a new rookie partner fresh out of the academy, who spontaneously combusts upon his introduction.
- Arcee of Transformers Prime has, as of now, lost two of her partners to Decepticons, both rather horrifically. This has left her with little patience, but great protectiveness, for her newest partner: the human Jack. It took a near death experience for her to accept him as such. Unfortunately for Arcee, her anger at the Decepticons who killed her partners (particularly Airachnid) can make her prone to acting rashly. And the 'cons aren't above using this against her.
- Max Tennyson lost his partner Devin Levin to the alien Ragnarok in Ben 10. Season 3 of Ben 10: Alien Force has an episode focusing on this event and Devin's son Kevin attempting to get revenge for his death.
- The Simpsons: A Running Gag in the show are snippets from the fictional McBain movie, essentially an Arnold Schwarzenegger parody. Grizzled Cowboy Cop McBain loses his old partner in a hit arranged by his arch-enemy, the drug-dealing Senator Mendoza. For bonus points, the partner in question is A] black, B] due to retire in a few days, and C] shows McBain a picture of his family right before he dies.