Little John: Let's face it: you gotta be a man to wear tights!
Sid in Toy Story wears all black, tortures his toys and terrorizes his sister, but in his sleep, dreams about "riding the pony". A subversion, because he has plainly not yet learned to embrace that side of himself.
In Toy Story 3, Lotso is a purple teddy bear who runs Sunnyside with an iron fist, and the clothing-obsessed Camp Straight Ken is one of the toughest guards.
In The Longest Yard rugged, badass, and violent inmate Torres (played by the wolfish Lobo Sebastian) shamelessly enjoys watching... Joy Behar's all-female talk show The View, much to the other prisoners' surprise.
Grossberger, the hulking mass murderer in Stir Crazy. While feared by the entire prison, Grossberger proves to be a softy, quickly befriending the main characters and singing a soulful rendition of "Down in the Valley." Actor Erland Van Lidth was himself a bit of real-life version of this trope, being a professional wrestler as well as a classically trained bass-baritone opera singer.
Briefly toyed with in Ocean's 13, when Rusty and Danny Ocean watch Oprah and get a little choked up.
Rufus Excalibur ffolkes in North Sea Hijack is a misogynist counter-terrorism and hostage rescue expert and trainer... and keeps cats and does needlepoint embroidery of kittens to while away the time. It might have had something to do with being raised by a maiden aunt along with his five older sisters.
In Demolition Man, John Spartan was implanted with the skills and desire to knit during his prison sentence. He knits a sweater for Huxley as an apology and feels sheepish about it. Played totally straight in that it's stated in the movie that he was given sewing skills because it was determined he had an aptitude for sewing, and would enjoy it if he learned how. It also becomes a plot point of all things, as Cryo-Prison inmates are implanted with different skills so they would be "useful to society" when they were thawed. And while Spartan got knitting, his nemesis Simon Phoenix got computer hacking so he could help the real villain overthrow the rebel leader.
In In Like Flint superspy and ultimate ladies man Flint doesn't just like ballet, he teaches and performs it.
In the New Zealand film Stickmen (it's about Pool sharks, FYI), there is a character who frequents all the hardest bars wearing a bright pink shirt. No one mocks him for this...cause he's an Ax Crazy martial artist who's happy for any reason to beat seven shades out of any fool he finds, and a pink shirt is prime Schmuck Bait.
Arnold Schwarzenegger's character in Red Heat is a tough Russian policeman with a pet parakeet. He becomes enraged when someone inadvertently suggests a parakeet might be better for a young girl.
In the original Angels In The Outfield Pirates manager Duffy McGovern (Paul Douglas) is a foul-mouthed bully who'd make a good "Before" poster boy for anger management. He also has a pet parrot named Joe who says "Good morning" politely. He says he only swears when he's annoyed, and Joe never annoys him.
The opening shot of Pumping Iron shows Arnold Schwarzenegger and his training buddy Franco Columbu in a ballet class.
In the Jude Law remake of Alfie, he says at one point "If you ooze masculinity, like some of us do, you have no reason to fear pink. "
In Get Shorty, Ray "Bones" Barboni spends much of the movie dressed in a hot pink blazer and seems to have an affectation for pastel clothing in general. Bo Catlett, meanwhile, has a pink toilet and obsesses over his pristine white carpet. They're the villains of the piece, and very brutally violent people.
In The Rundown, the Rock's character is a bounty hunter who wants to open a haute cuisine restaurant.
Mr Hollands Opus: The football coach minored in Modern Dance in college and choreographs the big dance number in the school musical. He also shrewdly uses it, because people will be expecting his football players, who he trains for the play, to be stumbling all over the stage and will come for the spectacle... but be blown away by the dancing.
Mr And Mrs Smith: John listens to Air Supply and graduated from Notre Dame with an art history degree.
In Mike Myers' comedy The Love Guru, tough hockey player aptly-named Jacques "Le Coq" Grande, whose personal logo is a rooster, seduces Prudence Roanoke by singing a Celine Dion song, cooking pizza, fondling and caressing her all the time and generally being so nice to her that other men in his place would look like wussies by comparison. While he did not.
In Iron Man 2, Vanko apparently likes cockatoos—to the point that one of his conditions for working for Justin Hammer is "I vant my boord", and when Hammer gets him one that isn't the one he had to leave behind in Russia, he is annoyed—but ends up taking the bird anyway.
The film version of The Hobbit has Dori and Ori, who both have rather "effeminate" mannerisms, such as knitting, drawing and drinking tea/wine, although this may be due to them being the "toymakers" or other non-warrior dwarves Balin refers to. It doesn't help that Dori is very motherly towards Ori, either.