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Anime And Manga
- Horribly averted in Fullmetal Alchemist. Even though the popular opinion is that alchemy should be used for the good of the people, the corrupt government (specifically President Evil Bradley) ordered the State Alchemists to annihilate the nation of Ishval, even when they tried to surrender! It's partially for this reason that State Alchemists are shunned for being dogs of the government, so in a sense, this trope can be felt in the atmosphere.
- The eponymous characters of Claymore aren't allowed to kill humans, even if it's to protect other humans. Any Claymore who does so is sentenced to death. And yet Awakened Beings are allowed to do as they please. Then again Awakened Beings aren't really part of the organization anyway.
- One plot point from the 4th War Arc of Naruto is guarding the daimyo, the Muggle leaders, because since they are the ones ninja answer to, capturing them will let Tobi put pressure on the Allied forces to hand over the 8 Tails and 9 Tails.
- The mages from Fairy Tail form guilds to take odd jobs, but it is illegal for a guild to accept an assassination mission. Thus there are many illegal guilds that the government has such a hard time keeping down that they hardly even bother.
- According to the bus driver in Rosario + Vampire, monsters aren't allowed to harm humans for any reason, even to save their own. Yet the Student Police are convinced it's okay to execute any human who discovers Youkai Academy. By the way, the school's supposed to teach coexistence. When you consider that the leader of the Student Police, Kuyou, is a spy for the Fantastic Racism organization Fairy Tale, it makes a bit more sense.
- Blood-C: Due to a pact she made with an unknown person, Saya is literally incapable of harming humans for any reason. The Elder Bairns are fair game, however.
- Animorphs: the group never kills Yeerk-infested humans, but has no such restraint against Hork-Bajir or Taxxons. This later backfires on them when Visser One realizes only humans wouldn't kill humans, as Andalites would have had no issue killing them.
- In most Asimovian fiction, this rule is in full effect with Three-Laws Compliant robots; the first law is that they cannot harm humans, nor allow a human to come to harm. Many of his stories are about how this general rule fares in specific circumstances.
- Forcefully imprison a human as long as the human is not physically harmed.
- Lie to them to avoid hurting their feelings only for the lies to cause them greater emotional harm.
- Arrange for them to be fired from their job, so long as they can arrange for another job.
- One set of robots didn't have the second part "nor allow a human being to come to harm" and so they could arrange for an "accident" and then refrain from saving them from that "accident".
- In the Discworld novel Sourcery we're told that wizards don't kill non-magic users because 1) They seldom notice them 2) It's not sporting, and 3) Who'd do the cleaning up and prepare meals and so on.
- In Harry Potter, there are a lot of laws in place to protect the muggle population from physical harm (though mindwiping them to uphold the Masquerade is government policy), which are frequently alluded to. The punishment for killing a Muggle is the same as that for killing a wizard. The Death Eaters throw them out the window the first chance they get.
- Shadowhunters and Downworlders in The Mortal Instruments are forbidden by the Law to hurt mundanes.
- In Twilight, the Cullens are vampires who adhere to a strict "vegetarian" diet which means they only drink the blood of animals and not humans. Unfortunately, this doesn't mean they're required or able to stop OTHER vampires from killing the humans they themselves won't eat.
- In Mage: The Ascension this is a big deal for both the Traditions and the Technocracy, as both have strict rules about not letting "sleepers" become casualties of The Ascension War. Both factions may treat that rule as. . .flexible. . .at times, but it's still officially a major rule for both sides.
Live Action Television
- In Buffy the Vampire Slayer
- Buffy sometimes fights against "normal" humans, but usually tries not to kill them. In one instance, accidentally "killing" a human-looking robot in self-defense is enough to give her a Heroic BSOD.
- The concept is explored further when (other vampire slayer) Faith accidentally kills a human while in the middle of a vampire fight. Giles tries to explain that such accidents are, historically, not uncommon for Slayers. In the grand scheme of things, it's seen as a small price to pay for all the vampires, demons, and other nasties that a Slayer kills on a daily basis.
- In Grimm, Grimms (or at least the ones we know like Nick and Trouble) try not to kill normal humans, it even became a plot point after Nick accidentally kills one during a supernaturally-induced rage. Renard even points out the fact that his guilt was a double standard.
- The Unknowns from Kamen Rider Agito are forbidden from killing normal humans (their MO is killing those with special powers). One unlucky Monster of the Week had this demonstrated on them the hard way.
- Season 4 of True Blood gives a partial example of this trope as the Vampires attempt to recover from Russel Edgington's stunt on live television. All the vampires are strictly forbidden from killing/horribly maiming humans... if there's a chance they can be caught on camera.
- In the Supernatural episode "Freaks and Geeks", Dean stops Krissy from killing Victor, The Man Behind The Monsters who killed her father, because he's entirely human and "we don't kill people". Despite Dean himself having killed or tried to kill evil humans in a number of other episodes.
- If, in the Assassin's Creed series, you repeatedly kill civilians, your "Synchronization Meter" (Health Bar) goes down. If you kill more than three civilians in a short frame of time you desynchronize. The reason given is because The Protagonist (Ezio or Altair, usually) never killed anyone because it would go against the first tenet of the Creed. This is averted after beating the game, letting you kill anyone you want, especially those annoying beggars, without any ill effect.
- The demons in the Reincarnation series aren't allowed to kill any humans other than Reincarnies, however much they may want to. They'll always mention to the player if a method being attempted risks harming innocents.