Toradora! - Taiga, after torturing Ami by forcing her to do multiple impersonations, uses threats of impersonations to come in order to control her. The best of the bunch? "Mona Lisa taking a corner at 250 miles per hour." Just picture it for a minute and TRY not to laugh.
In the Part 4 of JoJo's Bizarre Adventure, Diamond is not Crash/Diamond is Unbreakable, the main villain's unhealthy fascination with (severed) hands starts after seeing a Mona Lisa painting.
The BatmanElseworld "Dark Masterpiece" parallels a modern art-theft (by the gallery owner) being prevented by Batman with the kidnapping of Lisa del Giocondo herself (by her husband) being prevented by Leonardo's apprentice in a Renaissance-style bat-costume. It ends with Leonardo asking Lisa what her secretive smile is about, and being told that she's pregnant, and he's the father.
In Booster Gold, the Mona Lisa is Booster's sister. Booster comes back to get his sister and tells Leonardo he's taking her back to Rome. Leonardo, who had the face all done, struggled to grasp the rest of her after she left. He decided, due to personal preference, to give her dark hair and change her clothing because she had been wearing her costume while he was drawing... and that would have looked stupid.
In October 2011, Marvel did a series of vampire variant covers to tie in with X-Men: Curse of the Mutants. The cover for S.H.I.E.L.D. #4 (in which Leonardo is a main character) was a vampire Mona Lisa◊.
Cubitus: One gag has Semaphore painting a copy of the Mona Lisa. Cubitus suggests some alterations and paints his own face over it.
One Donald Duck comic had the poor duck nigh-lovestruck by the Duck Universe Mona Lisa equivalent. It soon turned out that Donald's ancestor, or previous incarnation perhaps, was Leonardo's helper. Mona Lisa herself was a duck of really nasty disposition, which made painting her favorably problematic. After many futile attempts, what finally got the smile on her face was the sight of Renaissance Donald and Leonardo getting into a fight after the former wrecked the latter's laboratory.
At the beginning of Hudson Hawk Leonardo da Vinci is shown with the Mona Lisa, which is finished except for the mouth. Then we see why: when the model opens her mouth, she has very bad teeth. The joke is that Leonardo decided to give her the enigmatic smile so he wouldn't have to show the teeth.
In Equilibrium, Mona Lisa is one of the paintings which is captured and burned in the opening. Adding to the tragedy is that the main character, initially a ruthless enforcer of the totalitarian regime, has no idea about the significance of it.
Parodied outright in Asterix & Obelix & Cleopatra. To their credit, Monica Bellucci makes a hot Mona Lisa.
In the Spanish short film La Gioconda Esta Triste (The Mona Lisa is Sad) the Mona Lisa stops smiling, and so does every single painted, drawn, photographed or videotaped reproduction of the painting. Not only that, but at the same time everyone on Earth also forgets how to smile. Against a background of international tension, environmental destruction and terrorist threats scientists try to solve the mystery. They finally conclude that the Mona Lisa's sadness represents that of the entire world, and only way to save the world is for everybody to try to smile again. But it's too late - everybody starts weeping, and the world ends.
Ever After - Leonardo shows off his latest work (the famous painting, inaccurately depicted as on a medium that can be rolled and stored in a tube) when introducing himself.
Crowley in Good Omens posesses an early sketch of the Mona Lisa, which he thinks is superior. Apparently, Leonardo agreed:
"I got her bloody smile right in the roughs," he told Crowley, sipping cold wine in the lunchtime sun, "but it went all over the place when I painted it. Her husband had a few things to say about it when I delivered it, but, like I tell him, Signor del Giocondo, apart from you, who's going to see it? Anyway ... explain this helicopter thing again, would you?"
There's a passage in The Godfather where Lucy Mancini, after Sonny Corleone's death, tells her new lover about her affair with Sonny. When she says, "I did everything with Sonny," she's got what the new guy thinks of as a Mona Lisa Smile, and he can't help wondering if this explains what La Gioconda was smiling about.
In the Doctor Who serial "The City of Death" the villain gets Leonardo to paint several copies of the Mona Lisa so he (the villain) could sell them multiple times to facilitate his evil plot. The Doctor foils this plan by writing "This Is A Fake" in felt tip on the boards Leonardo uses. Naturally, this means the one hanging in the Louvre also has "This Is A Fake" written under the painting, but the Doctor says that if people have to x-ray it to know if it's any good, they deserve to think it's a fake.
Mona is taken out of her portrait so Alex can cheat on a test in Wizards of Waverly Place. She ends up going back in wearing one of Harper's home made necklaces.
This concert poster for Frank Zappa and the Mothers of Invention. Referred to as the Mona Zappa.
In Spaceship Earth at Epcot, one scene is the lady posing for the painting and rapidly losing her patience because Leonardo keeps on getting distracted by other projects.
In the card game Chrononauts, there are a set of three Artifact cards: Mona Lisa (Obvious Forgery), Mona Lisa (Excellent Forgery) and Mona Lisa (Real Thing). When more than one is in play, the Excellent Forgery trumps the Obvious Forgery, while the Real Thing beats both.
One scene in Panic takes place in a museum displaying the Mona Lisa. Among the ways you can mess with her are making her comically obese, turning her into Medusa (leading to Slap getting petrified) or a werewolf, or causing a mountain climber to emerge from her cleavage and climb his way up her face to the top of her head.
Rusty and Co. has a painting whose eyes followed you (for a good reason). It managed to confuse umber hulks. See also the next page.
In the Pepe Le Pew cartoon "Louvre Come Back To Me", Pepe pursues his hapless female victim through the Louvre, finally chasing her through the museum air-vents. His distinctive aroma drifts through the museum, driving assorted masterpieces to Wild Takes. The last shot is the Mona Lisa, who quips to the audience "I can tell you chaps one thing, it's not always easy to hold a smile."
In Courage the Cowardly Dog, the family visits the Louvre, and Muriel ends up being trapped in the Mona Lisa when a series of events causes all of the works of art to come alive.
In her first appearance (of two) on Darkwing Duck, Splatter Phoenix steals the Mona Lisa's smile with intent to ransom it back.
All the works of art stolen by The Collector in the Sushi Pack episode "But is it Art?" were parodies of famous paintings, including "The Groaning Pizza," a pizza-faced person in Mona Lisa's pose, but with a distinct frown.
On Time Squad, they used the joke that she had very bad teeth which is why Leonardo decided to give her the smile so her teeth wouldn't show.
"Treehouse Of Horror I": In the third segment ("The Raven") the painting of Lenore features Marge in a Mona Lisa pose.
"Treehouse Of Horror IV": One of the paintings in the background during Bart's introduction features Marge in a Mona Lisa pose.
In the third segment of "Treehouse Of Horror IV" Chief Wiggum and his police corps destroy all museums "to prevent mummies from rising". Lou throws a copy of the Mona Lisa on the fire, to which Wiggum simply says: "Nice work, Lou."
In the animated version of Where In The World Is Carmen Sandiego?, the titular thief starts stealing various facial features from famous works of art. When she's got everything but the mouth, Zack asks: "who has the most famous smile in all the world?" The answer is of course, this trope (and not "Tom Cruise" as Ivy dreamily suggests).