The Running Gag of FRAU BLUCHER!(Dramatic thunderclap, horses whinny in fear).
Especially Eye-gor just popping out and shouting "Blucher" (*WHINNY*) to make the horses freak out, and Marty Feldman's impish grin.
In The Musical, the horses whinny when she just mouths her name.
Frederick tells the others not to let him out of the room while he's there alone with the monster, no matter what. Naturally, the instant the monster wakes up..
Frederick: Let me out, let me out of here, get me the hell out of here. What's the matter with you people?! I was joking! Don't you know a joke when you hear one?! HA-HA-HA-HA! Jesus Christ, get me out of here! Open this goddamn door or I'll kick your rotten heads in! Mommy!
'Y'know, I'll never forget my old dad. When these things would happen to him, the things he'd say to me..."
The best part of that is when he nods at the end, like it was a pearl of wisdom.
"Now, let us all go to my house for a little wine, a little spongecake- [the monster accidentally pulls Kemp's wooden arm off] Und shit! [regards his arm for a second, then dramatically points to the door with it] To the lumberyard!"
You know how funny this movie is? According to the commentary, Mel Brooks had to stick a handkerchief in his mouth when filming most of the scenes so he wouldn't ruin them by laughing from behind the camera. He had to do the same for the entire crew.