Aunt Sarah, especially to people who aren't dog lovers. It's possible she's just prejudiced against dogs and doesn't like them. It's also possible she didn't want to allow any animals near the baby, since she did leave her cats downstairs.
The alligator in the zoo. Trying to eat Lady or trying to help remove the muzzle - and simply not realising his mouth was too big.
Designated Villain: The dog catcher is perhaps the least evil of the Big Bad Ensemble; his job is to find dogs and put them in his pound to await pickup, but the film generally portrays him as antagonistic for this, particularly when he agrees to have Tramp put to sleep. Also, though she may be particularly harsh towards Lady and the Tramp, Aunt Sarah does what she does out of concern for others, particularly for her cats (who get Lady muzzled with a Wounded Gazelle Gambit) and for Darling's child. Plus, she hates runaways (and also, it would seem, strays), to the point of chaining Lady to the backyard doghouse to teach her a lesson, and around the time she arranges for Tramp's euthanasia, she's only aware of two things as far as the surrounding circumstances were concerned: one, that the child's life was in danger, and two, that two dogs, one of them a stray, were at the scene.
Ethnic Scrappy: The infamous Siamese Cat duo. On top of being, of course, villains, their Ethnic Scrappy status manifests itself in their buck teeth and the annoyingly terrible grammar during their song.
Fair for Its Day: The ethnic stereotypes personified in animals, the cats in particular.
Narm: When the rat is bitten by Tramp the first time, it squeaks like a chew toy. (It squeaks normally every other time, not even close to narmy.)
Villain Has a Point: Aunt Sarah putting a muzzle on Lady. It's generally not a good idea to allow dogs near newborn children and Aunt Sarah believed Lady was violent and destructive. She was doing what she thought was best for the safety of her nephew. That said, she also somehow thought leaving two cats around unmonitored in the presence of a dog and baby would be safe.