Acting for Two: Of the main characters, Frank Welker voiced both Ray and Slimer. Welker would also voice a number of antagonists, such as the Bogeyman, Sandman and Samhain. Maurice LaMarche also voiced a number of roles, too. Lorenzo Music also voiced Peter's dad, with Dave Coulier taking over later. And since Laura Summer (who originally played Janine) is the only voice actress credited, she may well have played every female role in the series until she was replaced by Kath Soucie.
In "A Fright at the Opera", before the Diva selects Peter as her bodyguard, she states "I hate Mondays". Lorenzo Music's (Peter's voice actor) most famous role, Garfield, also has a distaste for that particular day.
Executive Meddling: Despite its runaway success, ABC decided they needed to "fix" the show and hired a consulting firm named Q5 to take a look at it. According to J. Michael Straczynski, the head writer at the time, Q5 didn't do any audience research or focus testing but demanded changes based on what they themselves believed would made a cartoon successful. Janine was changed to be more feminine and meek, and received round glasses because it was claimed her pointy ones could scare children. More changes were forced upon the writing staff starting with Season 3. The Junior Ghostbusters, a group of recurring child characters, were added. More focus was placed on Slimer, who spun off into his own (doomed) cartoon in Season 4. The whole team ended up taking on fixed roles: Egon was "The Brain", Ray was "The Hands", Peter was "The Mouth" and Winston was "The Driver" (which many of the writers noted the Unfortunate Implications of). This was enough to make Straczynski leave his position after Season 3 (though he wrote some later scripts for the show as a free-lance writer).
The only reason the Egon/Janine relationship was never allowed to go anywhere? Harold Ramis hated the idea, and since the actors had creative control over their characters, it was vetoed. This is why it was totally dropped in Ghostbusters II for a Janine/Louis relationship.
Q5 had also wanted Straczynski and the other writers to ditch Ray, as they felt he was a pointless character. Ray had always been the warm, friendly one, but by this time, the other cast had been rendered so safe and bland, they decided he was redundant. However, this was one change that was quickly and successfully vetoed.
One of the most jarring changes was Peter could no longer be mean to Slimer.
The Other Darrin: After the syndicated episodes, Dave Coulier replaced Lorenzo Music as Peter and Kath Soucie replaced Laura Summer as Janine. A season later, Arsenio Hall left for his talk show and was replaced by Buster Jones.
In the second season, whenever the Stay Puft Marshmallow Man appeared, Frank Welker handled any vocal effects. In Season 3's "Sticky Business," John Stocker took over the role. Later in Season 5's "Partners in Slime," Welker again voiced the character for a brief cameo.
Show Accuracy/Toy Accuracy: Strangely, the Kenner toy line had very few ghosts who had actually appeared in the show. Exceptions to this were often lacking in accuracy: the 'Slimer' figure was named Green Ghost and looked more like his film counterpart, having been designed and marketed before the show had even come up with his name; and the original Stay Puft figure is about the same height as the 'busters themselves (the latter was eventually reissued as a plushie that was more accurately sized relative to the Ghostbuster figures- not that it would be physically possible to make a toy of the character to scale with the figures what with the character having been about 100' tall, and even today with a 2' tall Mattel foam figure or Diamond Select coin bank, it's still not quite happened). The likenesses of the Ghostbusters themselves also shares a bit with the early demo reel(as described below) which is why the Ray figure is so fat and why Winston's face is off-model.
Trendmasters would eventually make a toy of Samhain in the Extreme Ghostbusters line... albeit rather off-model to his animated look.
Mattel did release a series of 9" action figures with much improved likenesses around the year 2010, although it only saw 6 toys, but it did feature a proper Samhain, FINALLY. Art Asylum also released a line of Minimates that tied in with both the film and cartoon which featured a few ghosts in toy form for the first time.
Take That: Dr. Basingame, a charlatan spiritualist first appearing in "The Spirit of Aunt Lois", wore a safari jacket and camera around his neck in that episode - an awful lot like the wardrobe of Jake Kong from Filmation's Ghostbusters. This was deliberate; Word of God said Basingame was a deliberate swipe against the rival show. On that note, another episode had a brief insult aimed at He-Man, which was animated by Filmation as well.
A later episode was dedicated at jabbing at the Teenage Mutant Ninja Turtles with 3 rude ghosts that ate pizza- the Turtles own cartoon was trashing Ghostbusters in the ratings at the time. Cam Clarke, the voice of Leonardo in the original TMNT cartoon, even voiced one of them.
Technology Marches On: Thanks to the corporate espionage perpetrated by Paul Smart, a super-advanced, AI-driven Robo-Buster X1 was set to put the "dinosaur" Ghostbusters out of business. At its unveiling, the robot revealed it had an incredible 20MB of on-board memory.
Ernie Hudson (Winston in the movies) auditioned for his own character.
In light of falling ratings after the Slimer Re Tool, producers asked JMS to return as head writer for Season 6. JMS had other projects at the time, though he agreed to write a few episodes (chief among them "Janine, You've Changed").
The executives softened Janine's character to be more "motherly" but they also wanted to make her a full-fledged Ghostbuster as opposed to just a secretary who only occasionally got involved with the plot. Quite a few fans think this might have actually been a good idea, especially if it had been thought up during the show's peak.
An early four minute demo reel featured an entirely different character design for Peter Venkman (one that came closer to the more blue collar looks of live action Venkman Bill Murray than the chiseled pretty boy design they ultimately went with), the Ghostbusters wearing their beige movie jumpsuits instead of the multi-color coded jumpsuits they wore in the finished series, and Slimer still appeared to be an antagonist rather than a team pet and mascot.