In "The Last Train to Oblivion", the ghost of Casey Jones was able to pass on after managing to prevent a train crash. Now, there was no danger of a train crash until Casey kidnapped Peter and started the stolen train down the tracks in the first place... but the problem wasn't that he had to avert a crash. It was that he had to perform his legendary, spectacular run, pushing the engine beyond the limit, doing what was thought to be impossible... AND not crash. It wasn't penance; it was post-mortem OCD.
In "No One Comes to Lupusville" the boys visit a town full of vampires and werewolves. Three dozen episodes later they're hired by a Boldavian count who claims to be the last vampire, and no one gainsays him. Guess we know who ultimately won the battle in Lupusville ...
To be fair, we do see more vampires later in the series. So the count is basically just being emo.
Or he's just the last vampire in Boldavia.
In "Three Men and an Egon," upon returning to his adult self, Egon is asked if he really doesn't remember what just happened to him. He doesn't... because humans don't start really forming memories until they're anywhere between 3 and 5 years old, and Egon spends the majority of the episode as a baby that's probably barely a year old, if that.
In the episode "Janine, You've Changed" it's hinted that Janine's "Fairy Godmother" haunted Cleopatra and Marilyn Monroe. What did these women have in common? Both were praised for their beauty to the point that their intellect was overlooked. Both look different in various images of them. (The RL explanation being plastic surgery and bleached hair for Marilyn and artistic interpretation for Cleo.) Both women are believed to have committed suicide.
In the first episode, "Ghosts R Us", the Class 10 runs over a bunch of cars using its bike. What's disturbing is that we only see one car have its passengers safely get out before getting destroyed. Did this entity just kill dozens of innocent people before it was destroyed?
J. Michael Straczynski explicitly notes this on the commentary for that episode, saying that the network never let that happen again.
In "The Cabinet of Calamari," when the disappearing victim escapes from the cabinet, he asks for some money to call his wife. Winston informs him that since he's been gone, the cost of payphones has increased. The man was in there for years without aging or noticing the passage of time, and chances are his wife has aged or moved on without him.
In "Buster the Ghost," the titular ghost decides to kill himself after yet another failure at a job, this time as a tooth fairy. As he says this, he makes a Finger Gun gesture pointing at his head. As shown in other episodes, ghosts can shot with their hands in that gesture. It may have been possible that he really was going to commit suicide within a few seconds if he hadn't been interrupted by his talking watch.
In "You Can't Take It With You," after the team break into the mansion of an insane millionaire teleporting his money into the Spirit World so he can keep it after he dies, they find and release three of his employees. The prisoners say their boss "was going to put us in that terrible machine" after he finished with his fortune, but given that: the guy is old, frail, and gets around via motorized wheelchair, and had no way of forcing them into his machine; the building was otherwise empty, as is stated outright later; they were locked in an airtight room; and Egon explicitly finds the plan "very Egyptian," it seems more likely he was planning on sending them to the afterlife the old-fashioned way...
If "The Thing In Mrs. Faversham's Attic" was trapped up there for 70 years, why did it only start bothering Mrs. Faversham enough now to seek the Ghostbusters for help? Remember that Mrs. Faversham, and then the Ghostbusters, heard its rumbling and laughter before they got anywhere near the attic. That suggests that the Thing was already starting to break out of its prison. Given that Charles Faversham was so horrified at what he'd summoned that he renounced it and imprisoned it in the attic, it's probably for the best that we never find out what it would have actually done if it had freed itself.
The more feminine, mild-mannered Janine with the long straight hair, rounder glasses and softer, less regional voice from the Lighter and Softer season 3 forward - with a few other makeovers along the way - becomes incredibly disturbing after you see "Janine, You've Changed" and realize that she was secretly being manipulated and physically altered by a demon all along. By extension, pretty much all the Lighter and Softer episodes become a lot more disturbing when you realize what was happening with Janine and the demon.
The Containment Unit. It's a massive disaster waiting to happen. Any time it is shut down, the collection of horrible things, including ghosts, eldritch abominations, demons, and other assorted terrors, are moments from escape. Now, consider, the Ghostbusters won't be in business forever. They've almost put themselves out of business on numerous occasions. And even if you can count on newer ghosts and demons showing up, the Ghostbusters can't keep doing it forever. Even with Extreme Ghostbusters, you have to consider that at some point or another, there just might not be any replacement Ghostbusters. So what happens then? Does the cost and maintenance of the Containment Unit fall on the taxpayers? What if it goes forgotten, and the power shuts down? It would be akin to another 9/11 or a nuclear blast.
In the episode "Look Homeward Ray", Ray tells the townsfolk to bury the garbage trucks functioning as makeshift traps, and give the ghosts time to "deres". These ghosts are explicitly the souls of deceased people. That means that the effects of a trap on a soul over long terms has a degrading quality. Still want to call the Ghostbusters to capture the annoying ghost of a late relative living in the haunted house?
Several members of the Ghostbusters staff have been possessed or had their souls removed from their bodies over a period of time. One wonders if they need counseling afterwards for the feelings of violation and Mind Rape that must surely come from having an Eldritch Abomination inhabiting your body.