First Castlevania game to feature an American character.
First Castlevania soundtrack to be composed by Michiru Yamane, who would go on to be a regular composer for Castlevania soundtracks.
Currently, Castlevania Bloodlines is the only originalnote as in, not a remake, port, or retelling of a previous Castlevania game console Castlevania game that has not had a Virtual Console rerelease yet.
Official Fan-Submitted Content: Two enemies, a boss, and an obstacle were chosen from a contest for the Japanese exclusive MegaDriveFAN magazine. Those enemies were the green enemies in the gardens of Versailles called Headhunter Bats, the beetles in the Munitions factory (which also housed the winning trap, the rollers) called Flying Beetle Rollers, and the boss was the Silhouette Demon just before the Princess of Moss.